Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,494 T→C 8.2% intergenic (‑30/+22) PP_5715 ← / ← PP_5716 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,4940TC 8.2% 169.5 / 5.3 61intergenic (‑30/+22)PP_5715/PP_5716hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/28);  new base C (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

GGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAG  >  NC_002947/5491348‑5491639
                                                                                                                                                  |                                                                                                                                                 
ggTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCgtgt                                                                                                                                                 <  5:149784/149‑1 (MQ=255)
    aGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGa                                                                                                                                              >  8:13813/1‑148 (MQ=255)
     ggTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            <  5:151955/149‑1 (MQ=255)
       tAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAgg                                                                                                                                          <  2:19916/149‑1 (MQ=255)
         ggCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTGGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGc                                                                                                                                         <  5:185674/148‑1 (MQ=255)
          gCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcct                                                                                                                                       <  3:102839/149‑2 (MQ=255)
              ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcctgga                                                                                                                                    <  4:261284/148‑5 (MQ=255)
                  cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               >  6:49248/1‑149 (MQ=255)
                  cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               <  5:49248/149‑1 (MQ=255)
                      aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                           <  3:230416/133‑1 (MQ=255)
                      aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                           >  4:230416/1‑133 (MQ=255)
                       atctcAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                          <  7:204823/145‑1 (MQ=255)
                         tCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                                                                        <  7:13813/149‑1 (MQ=255)
                            aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                          >  8:204823/1‑144 (MQ=255)
                            aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTc                                                                                                                     >  6:276221/1‑149 (MQ=255)
                             aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            <  6:12958/125‑1 (MQ=255)
                             aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            >  5:12958/1‑125 (MQ=255)
                                    ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 >  8:290366/1‑145 (MQ=255)
                                    ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 <  7:290366/145‑1 (MQ=255)
                                    ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               >  8:12627/1‑131 (MQ=255)
                                    ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 <  1:115608/145‑1 (MQ=255)
                                    ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 >  2:115608/1‑145 (MQ=255)
                                            gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAc                                                                                                      <  5:276221/148‑1 (MQ=255)
                                               aaGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                   >  6:21187/1‑148 (MQ=255)
                                               aaGCACTGAAATTGTTGTCGATCAATACAAGGCTATTGCCGTTCAACCACAAAGAGTAATAGGCTAGCATAAATATTCACTACAAGACCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                   >  2:198725/1‑148 (MQ=255)
                                                  cACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcatatcatg                                                                                                   >  5:11276/1‑138 (MQ=255)
                                                         aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgt                                                                                        >  1:47974/1‑149 (MQ=255)
                                                          ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                                       <  6:168584/149‑1 (MQ=255)
                                                                   aTCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGttt                                                                              <  8:18831/149‑1 (MQ=255)
                                                                    tCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                             <  6:120988/149‑1 (MQ=255)
                                                                     cAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                            >  8:116814/1‑149 (MQ=255)
                                                                          ccAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCt                                                                       <  2:47974/149‑1 (MQ=255)
                                                                               cTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAAt                                                                  <  2:338496/149‑1 (MQ=255)
                                                                                 aTTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATgg                                                                >  2:307283/1‑149 (MQ=255)
                                                                                           aaCCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      <  5:21187/149‑1 (MQ=255)
                                                                                             ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                                    >  3:92927/1‑149 (MQ=255)
                                                                                                   aGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATc                                              >  2:311236/1‑149 (MQ=255)
                                                                                                     tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      >  3:170841/1‑139 (MQ=255)
                                                                                                     tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      <  4:170841/139‑1 (MQ=255)
                                                                                                       tAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAATTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggct                                          >  8:45548/1‑148 (MQ=255)
                                                                                                         aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggcgg                                         <  1:311236/148‑1 (MQ=255)
                                                                                                         aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCggcggc                                        >  7:55058/1‑149 (MQ=255)
                                                                                                               ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                >  7:98160/1‑87 (MQ=255)
                                                                                                               ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                <  8:98160/87‑1 (MQ=255)
                                                                                                               ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                   >  7:173396/1‑148 (MQ=255)
                                                                                                               ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                   >  8:308558/1‑148 (MQ=255)
                                                                                                                   aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                               <  7:299430/100‑1 (MQ=255)
                                                                                                                   aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                               >  8:299430/1‑100 (MQ=255)
                                                                                                                    tCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                             <  7:116814/149‑1 (MQ=255)
                                                                                                                      aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                           <  2:308365/149‑1 (MQ=255)
                                                                                                                       gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                          >  1:222641/1‑117 (MQ=255)
                                                                                                                       gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                          <  2:222641/117‑1 (MQ=255)
                                                                                                                                  aGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGcacca                 >  2:178127/1‑147 (MQ=255)
                                                                                                                                    gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  7:161459/1‑149 (MQ=255)
                                                                                                                                    gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  4:192106/1‑149 (MQ=255)
                                                                                                                                     cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAATTTCAACACCGCACCACAgg             >  7:150934/4‑148 (MQ=255)
                                                                                                                                     cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  2:242033/4‑148 (MQ=255)
                                                                                                                                      ccGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCa           <  3:192106/149‑1 (MQ=255)
                                                                                                                                             cGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTgg    <  7:308558/149‑1 (MQ=255)
                                                                                                                                              gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   <  8:161459/149‑1 (MQ=255)
                                                                                                                                              gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   <  4:92927/149‑1 (MQ=255)
                                                                                                                                               ccGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAg  <  3:288942/149‑1 (MQ=255)
                                                                                                                                                 gtgtCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   <  2:154481/146‑1 (MQ=255)
                                                                                                                                                 gtgtCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   >  1:154481/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAG  >  NC_002947/5491348‑5491639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑