Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,501 A→G 10.0% intergenic (‑37/+15) PP_5715 ← / ← PP_5716 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,5010AG10.0% 160.5 / 7.3 60intergenic (‑37/+15)PP_5715/PP_5716hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (2/4);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAGGTGCCACTGC  >  NC_002947/5491353‑5491649
                                                                                                                                                    |                                                                                                                                                    
ggTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                                      <  5:151955/149‑1 (MQ=255)
  tAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAgg                                                                                                                                                    <  2:19916/149‑1 (MQ=255)
    ggCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTGGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGc                                                                                                                                                   <  5:185674/148‑1 (MQ=255)
     gCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcct                                                                                                                                                 <  3:102839/149‑2 (MQ=255)
         ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcctgga                                                                                                                                              <  4:261284/148‑5 (MQ=255)
             cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                                         >  6:49248/1‑149 (MQ=255)
             cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                                         <  5:49248/149‑1 (MQ=255)
                 aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                                     <  3:230416/133‑1 (MQ=255)
                 aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                                     >  4:230416/1‑133 (MQ=255)
                  atctcAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                                    <  7:204823/145‑1 (MQ=255)
                    tCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                                                                                  <  7:13813/149‑1 (MQ=255)
                       aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                                    >  8:204823/1‑144 (MQ=255)
                       aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTc                                                                                                                               >  6:276221/1‑149 (MQ=255)
                        aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                                      <  6:12958/125‑1 (MQ=255)
                        aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                                      >  5:12958/1‑125 (MQ=255)
                               ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                           >  8:290366/1‑145 (MQ=255)
                               ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                           <  7:290366/145‑1 (MQ=255)
                               ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                                         >  8:12627/1‑131 (MQ=255)
                               ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                           <  1:115608/145‑1 (MQ=255)
                               ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                           >  2:115608/1‑145 (MQ=255)
                                       gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAc                                                                                                                <  5:276221/148‑1 (MQ=255)
                                          aaGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                             >  6:21187/1‑148 (MQ=255)
                                          aaGCACTGAAATTGTTGTCGATCAATACAAGGCTATTGCCGTTCAACCACAAAGAGTAATAGGCTAGCATAAATATTCACTACAAGACCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                             >  2:198725/1‑148 (MQ=255)
                                             cACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcatatcatg                                                                                                             >  5:11276/1‑138 (MQ=255)
                                                    aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgt                                                                                                  >  1:47974/1‑149 (MQ=255)
                                                     ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                                                 <  6:168584/149‑1 (MQ=255)
                                                              aTCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGttt                                                                                        <  8:18831/149‑1 (MQ=255)
                                                               tCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                                       <  6:120988/149‑1 (MQ=255)
                                                                cAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                                      >  8:116814/1‑149 (MQ=255)
                                                                     ccAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCt                                                                                 <  2:47974/149‑1 (MQ=255)
                                                                          cTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAAt                                                                            <  2:338496/149‑1 (MQ=255)
                                                                            aTTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATgg                                                                          >  2:307283/1‑149 (MQ=255)
                                                                                      aaCCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                                <  5:21187/149‑1 (MQ=255)
                                                                                        ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                                              >  3:92927/1‑149 (MQ=255)
                                                                                              aGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATc                                                        >  2:311236/1‑149 (MQ=255)
                                                                                                tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                                <  4:170841/139‑1 (MQ=255)
                                                                                                tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                                >  3:170841/1‑139 (MQ=255)
                                                                                                  tAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAATTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggct                                                    >  8:45548/1‑148 (MQ=255)
                                                                                                    aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggcgg                                                   <  1:311236/148‑1 (MQ=255)
                                                                                                    aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCggcggc                                                  >  7:55058/1‑149 (MQ=255)
                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                          >  7:98160/1‑87 (MQ=255)
                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                          <  8:98160/87‑1 (MQ=255)
                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                             >  7:173396/1‑148 (MQ=255)
                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                             >  8:308558/1‑148 (MQ=255)
                                                                                                              aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                                         <  7:299430/100‑1 (MQ=255)
                                                                                                              aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                                         >  8:299430/1‑100 (MQ=255)
                                                                                                               tCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                                       <  7:116814/149‑1 (MQ=255)
                                                                                                                 aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                                     <  2:308365/149‑1 (MQ=255)
                                                                                                                  gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                                    >  1:222641/1‑117 (MQ=255)
                                                                                                                  gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                                    <  2:222641/117‑1 (MQ=255)
                                                                                                                             aGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGcacca                           >  2:178127/1‑147 (MQ=255)
                                                                                                                               gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg                       >  7:161459/1‑149 (MQ=255)
                                                                                                                               gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg                       >  4:192106/1‑149 (MQ=255)
                                                                                                                                cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAATTTCAACACCGCACCACAgg                       >  7:150934/4‑148 (MQ=255)
                                                                                                                                cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg                       >  2:242033/4‑148 (MQ=255)
                                                                                                                                 ccGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCa                     <  3:192106/149‑1 (MQ=255)
                                                                                                                                        cGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTgg              <  7:308558/149‑1 (MQ=255)
                                                                                                                                         gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa             <  4:92927/149‑1 (MQ=255)
                                                                                                                                         gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa             <  8:161459/149‑1 (MQ=255)
                                                                                                                                         acggcgtCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa             <  1:242033/144‑1 (MQ=255)
                                                                                                                                          ccGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAg            <  3:288942/149‑1 (MQ=255)
                                                                                                                                            gtgtCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa             <  2:154481/146‑1 (MQ=255)
                                                                                                                                            gtgtCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa             >  1:154481/1‑146 (MQ=255)
                                                                                                                                                    aGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTCTCTACAATGGCGCGAACTTTGTCGTATCGACGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAGGTGCCACTGc  >  7:305790/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAGGTGCCACTGC  >  NC_002947/5491353‑5491649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑