Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,497,004 T→A 6.1% L26Q (CTG→CAG)  cobL → precorrin‑6Y C(5,15)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,497,0040TA 6.1% 195.7 / 2.8 66L26Q (CTG→CAG) cobLprecorrin‑6Y C(5,15)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (28/34);  new base A (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CACTGTACCTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGC  >  NC_002947/5496856‑5497148
                                                                                                                                                      |                                                                                                                                                
cACTGTACCTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCct                                                                                                                                                  <  7:34534/149‑1 (MQ=255)
 aCTGTACCTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCctg                                                                                                                                                 >  6:256519/1‑149 (MQ=255)
  cTGTACCTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCctgc                                                                                                                                                <  7:299311/149‑1 (MQ=255)
        cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                          <  5:256519/149‑1 (MQ=255)
        cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                          <  2:79762/149‑1 (MQ=255)
        cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                          >  5:14226/1‑149 (MQ=255)
        cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCATCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                          >  1:284343/1‑149 (MQ=255)
         tgtgGGAGCGGGCGAGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcgc                                                                                                                                         >  2:178478/1‑149 (MQ=255)
             ggAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCAGCAGGgcgcgcc                                                                                                                                      <  7:45728/148‑4 (MQ=255)
              gAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGcc                                                                                                                                    >  2:47980/1‑149 (MQ=255)
                gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGATGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                  <  4:42402/149‑1 (MQ=255)
                gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                  <  7:134062/149‑1 (MQ=255)
                gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGG‑CCGGC‑‑GCGCCCTGCTGGGCGCTGGCCggg                                                                                                                                 >  3:216970/1‑149 (MQ=255)
                gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCAAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                  <  5:206090/149‑1 (MQ=255)
                 cGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCggg                                                                                                                                 <  8:122649/149‑1 (MQ=255)
                       tGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCg                                                                                                                           <  5:19170/149‑1 (MQ=255)
                          ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGCCAGCGCCCAGCAGGgcgcgc                                                                                                                                       <  4:33806/136‑3 (MQ=255)
                          ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGCCAGCGCCCAGCAGGgcgcgc                                                                                                                                       >  3:33806/1‑134 (MQ=255)
                          ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCgg                                                                                                                          >  6:309906/1‑147 (MQ=255)
                              gAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGccc                                                                                                                    >  4:156435/1‑149 (MQ=255)
                                aGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCcgc                                                                                                                  <  2:150537/149‑1 (MQ=255)
                                aGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCcgc                                                                                                                  <  2:145361/149‑1 (MQ=255)
                                  gggCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                <  6:14226/149‑1 (MQ=255)
                                  gggCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                >  6:158413/1‑149 (MQ=255)
                                      gcgAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGG‑CCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                           <  4:216970/149‑1 (MQ=255)
                                       cgAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                           <  7:123391/149‑1 (MQ=255)
                                        gAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTg                                                                                                          <  2:284343/149‑1 (MQ=255)
                                           gCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGcc                                                                                                       <  5:158413/149‑1 (MQ=255)
                                                cccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTtgct                                                                                                  <  8:262069/149‑1 (MQ=255)
                                                 ccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                >  5:36250/1‑134 (MQ=255)
                                                 ccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                <  6:36250/134‑1 (MQ=255)
                                                   aaaaaTGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGcc                                                                                               >  4:25453/1‑149 (MQ=255)
                                                          gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTgg                                                                                                                                             <  4:130087/96‑1 (MQ=255)
                                                          gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTgg                                                                                                                                             >  3:130087/1‑96 (MQ=255)
                                                          gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCGGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                        <  6:271787/149‑1 (MQ=255)
                                                            ggATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGTCAGCTGGCCTTGCTGCCACGTTGCGt                                                                                      <  1:178478/149‑1 (MQ=255)
                                                               ttGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGAc                                                                                   >  8:205292/1‑149 (MQ=255)
                                                                  aaaTACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGc                                                                                >  3:198218/1‑149 (MQ=255)
                                                                       cATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGaa                                                                            <  2:285053/148‑1 (MQ=255)
                                                                           acacCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGTCCGCTGAACAGtt                                                                       <  1:69337/149‑1 (MQ=255)
                                                                            cacCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGTACAGTtg                                                                      <  5:325547/149‑1 (MQ=255)
                                                                             acCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTCAACAGTtgg                                                                     >  5:226958/1‑149 (MQ=255)
                                                                                   gCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTtggcctggc                                                               >  8:182087/1‑149 (MQ=255)
                                                                                    cTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTtggcctggcc                                                              <  7:182087/149‑1 (MQ=255)
                                                                                       aCAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAg                                                           <  1:47980/149‑1 (MQ=255)
                                                                                       aCAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAg                                                           >  5:201310/1‑149 (MQ=255)
                                                                                           tagtagGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                           >  1:285118/1‑97 (MQ=255)
                                                                                           tagtagGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                           <  2:285118/97‑1 (MQ=255)
                                                                                                  cATCGGGGTAGACGGCTTCAGCGGCCTGGGCAAACAGGGCCGGG‑‑GGGGCCTGCTTGGCGGTGGGCGGGGCGTTGGGAGCCCGCGGCATGTGGGCTTGGTGCCACGTTTCGGGTCCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGc                                                <  6:226958/149‑1 (MQ=255)
                                                                                                  cATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGc                                                >  2:287600/1‑149 (MQ=255)
                                                                                                   aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                        <  1:247194/108‑1 (MQ=255)
                                                                                                   aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                        >  2:247194/1‑108 (MQ=255)
                                                                                                   aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCt                                               >  2:116811/1‑149 (MQ=255)
                                                                                                   aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCt                                               >  3:126405/1‑149 (MQ=255)
                                                                                                      ggCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCGGGGCGCTGGCCGGGTGTTCGGCAGCCCGCGCCCGCGGGCCTTGCTGCCACGTTGCGTGACCGCGGGACAGGTGGCCTGGGCCAGCCCGTTGGAGCTGGc                                            >  4:34064/1‑149 (MQ=255)
                                                                                                      ggCGAAGACGGCTTCAGCGGCCAGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGc                                            >  2:35980/1‑149 (MQ=255)
                                                                                                            gACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGt                                      >  5:156192/1‑149 (MQ=255)
                                                                                                                gCTTCAGCGGCCTTGGCAAACCGGCCCGGC‑‑GCGCCCTGCTGGGCGCGGGCCGGGTCTTCGGCAGCCCGCGCCAGCGGGCCTTGCTGCCACGTTGCGGGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTg                                  <  3:84266/149‑1 (MQ=255)
                                                                                                                 cTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTgg                                 >  5:159209/1‑149 (MQ=255)
                                                                                                                    cAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGc                                <  7:205292/147‑1 (MQ=255)
                                                                                                                    cAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGc                                <  4:198218/147‑1 (MQ=255)
                                                                                                                         gCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTcgc                         <  6:201310/149‑1 (MQ=255)
                                                                                                                            tGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCgcggc                      >  7:72101/1‑149 (MQ=255)
                                                                                                                                 aaaCAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGcc                 >  1:330695/1‑149 (MQ=255)
                                                                                                                                           ggccagcgcCCAGCAGGGCGC‑‑GCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtg       >  4:64568/6‑149 (MQ=255)
                                                                                                                                                 cgcCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTgg   <  2:330695/149‑1 (MQ=255)
                                                                                                                                                  gcCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGc  <  3:156435/149‑1 (MQ=255)
                                                                                                                                                    ccTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtgc      >  1:99595/1‑143 (MQ=255)
                                                                                                                                                    ccTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtgc      <  2:99595/143‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
CACTGTACCTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGC  >  NC_002947/5496856‑5497148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑