Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,497,007 T→A 6.1% L27Q (CTG→CAG)  cobL → precorrin‑6Y C(5,15)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,497,0070TA 6.1% 198.5 / 2.6 66L27Q (CTG→CAG) cobLprecorrin‑6Y C(5,15)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (27/34);  new base A (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGCCAGCGGT  >  NC_002947/5496864‑5497155
                                                                                                                                                 |                                                                                                                                                    
cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                                 <  2:79762/149‑1 (MQ=255)
cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                                 >  5:14226/1‑149 (MQ=255)
cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                                 <  5:256519/149‑1 (MQ=255)
cTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCATCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcg                                                                                                                                                 >  1:284343/1‑149 (MQ=255)
 tgtgGGAGCGGGCGAGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGgcgc                                                                                                                                                >  2:178478/1‑149 (MQ=255)
     ggAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCAGCAGGgcgcgcc                                                                                                                                             <  7:45728/148‑4 (MQ=255)
      gAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGcc                                                                                                                                           >  2:47980/1‑149 (MQ=255)
        gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGATGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                         <  4:42402/149‑1 (MQ=255)
        gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                         <  7:134062/149‑1 (MQ=255)
        gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGG‑CCGGC‑‑GCGCCCTGCTGGGCGCTGGCCggg                                                                                                                                        >  3:216970/1‑149 (MQ=255)
        gCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCAAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCgg                                                                                                                                         <  5:206090/149‑1 (MQ=255)
         cGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCggg                                                                                                                                        <  8:122649/149‑1 (MQ=255)
               tGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCg                                                                                                                                  <  5:19170/149‑1 (MQ=255)
                  ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGCCAGCGCCCAGCAGGgcgcgc                                                                                                                                              <  4:33806/136‑3 (MQ=255)
                  ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGCCAGCGCCCAGCAGGgcgcgc                                                                                                                                              >  3:33806/1‑134 (MQ=255)
                  ccGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCgg                                                                                                                                 >  6:309906/1‑147 (MQ=255)
                      gAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGccc                                                                                                                           >  4:156435/1‑149 (MQ=255)
                        aGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCcgc                                                                                                                         <  2:150537/149‑1 (MQ=255)
                        aGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCcgc                                                                                                                         <  2:145361/149‑1 (MQ=255)
                          gggCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                       <  6:14226/149‑1 (MQ=255)
                          gggCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                       >  6:158413/1‑149 (MQ=255)
                              gcgAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGG‑CCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                                  <  4:216970/149‑1 (MQ=255)
                               cgAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                                  <  7:123391/149‑1 (MQ=255)
                                gAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTg                                                                                                                 <  2:284343/149‑1 (MQ=255)
                                   gCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGcc                                                                                                              <  5:158413/149‑1 (MQ=255)
                                        cccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTtgct                                                                                                         <  8:262069/149‑1 (MQ=255)
                                         ccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                       >  5:36250/1‑134 (MQ=255)
                                         ccAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCgcgc                                                                                                                       <  6:36250/134‑1 (MQ=255)
                                           aaaaaTGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGcc                                                                                                      >  4:25453/1‑149 (MQ=255)
                                                  gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTgg                                                                                                                                                    >  3:130087/1‑96 (MQ=255)
                                                  gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTgg                                                                                                                                                    <  4:130087/96‑1 (MQ=255)
                                                  gCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCGGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                               <  6:271787/149‑1 (MQ=255)
                                                    ggATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGTCAGCTGGCCTTGCTGCCACGTTGCGt                                                                                             <  1:178478/149‑1 (MQ=255)
                                                       ttGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGAc                                                                                          >  8:205292/1‑149 (MQ=255)
                                                          aaaTACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGc                                                                                       >  3:198218/1‑149 (MQ=255)
                                                               cATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGaa                                                                                   <  2:285053/148‑1 (MQ=255)
                                                                   acacCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGTCCGCTGAACAGtt                                                                              <  1:69337/149‑1 (MQ=255)
                                                                    cacCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGTACAGTtg                                                                             <  5:325547/149‑1 (MQ=255)
                                                                     acCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTCAACAGTtgg                                                                            >  5:226958/1‑149 (MQ=255)
                                                                           gCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTtggcctggc                                                                      >  8:182087/1‑149 (MQ=255)
                                                                            cTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTtggcctggcc                                                                     <  7:182087/149‑1 (MQ=255)
                                                                               aCAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAg                                                                  <  1:47980/149‑1 (MQ=255)
                                                                               aCAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAg                                                                  >  5:201310/1‑149 (MQ=255)
                                                                                   tagtagGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                                  <  2:285118/97‑1 (MQ=255)
                                                                                   tagtagGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCt                                                                                                                  >  1:285118/1‑97 (MQ=255)
                                                                                          cATCGGGGTAGACGGCTTCAGCGGCCTGGGCAAACAGGGCCGGG‑‑GGGGCCTGCTTGGCGGTGGGCGGGGCGTTGGGAGCCCGCGGCATGTGGGCTTGGTGCCACGTTTCGGGTCCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGc                                                       <  6:226958/149‑1 (MQ=255)
                                                                                          cATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGc                                                       >  2:287600/1‑149 (MQ=255)
                                                                                           aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                               <  1:247194/108‑1 (MQ=255)
                                                                                           aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGc                                                                                               >  2:247194/1‑108 (MQ=255)
                                                                                           aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCt                                                      >  2:116811/1‑149 (MQ=255)
                                                                                           aTCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCt                                                      >  3:126405/1‑149 (MQ=255)
                                                                                              ggCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCGGGGCGCTGGCCGGGTGTTCGGCAGCCCGCGCCCGCGGGCCTTGCTGCCACGTTGCGTGACCGCGGGACAGGTGGCCTGGGCCAGCCCGTTGGAGCTGGc                                                   >  4:34064/1‑149 (MQ=255)
                                                                                              ggCGAAGACGGCTTCAGCGGCCAGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGc                                                   >  2:35980/1‑149 (MQ=255)
                                                                                                    gACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGt                                             >  5:156192/1‑149 (MQ=255)
                                                                                                        gCTTCAGCGGCCTTGGCAAACCGGCCCGGC‑‑GCGCCCTGCTGGGCGCGGGCCGGGTCTTCGGCAGCCCGCGCCAGCGGGCCTTGCTGCCACGTTGCGGGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTg                                         <  3:84266/149‑1 (MQ=255)
                                                                                                         cTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTgg                                        >  5:159209/1‑149 (MQ=255)
                                                                                                            cAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGc                                       <  7:205292/147‑1 (MQ=255)
                                                                                                            cAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGc                                       <  4:198218/147‑1 (MQ=255)
                                                                                                                 gCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTcgc                                <  6:201310/149‑1 (MQ=255)
                                                                                                                    tGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCgcggc                             >  7:72101/1‑149 (MQ=255)
                                                                                                                         aaaCAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGcc                        >  1:330695/1‑149 (MQ=255)
                                                                                                                                   ggccagcgcCCAGCAGGGCGC‑‑GCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtg              >  4:64568/6‑149 (MQ=255)
                                                                                                                                         cgcCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTgg          <  2:330695/149‑1 (MQ=255)
                                                                                                                                          gcCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGc         <  3:156435/149‑1 (MQ=255)
                                                                                                                                            ccTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtgc             <  2:99595/143‑1 (MQ=255)
                                                                                                                                            ccTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTgtgcgtgc             >  1:99595/1‑143 (MQ=255)
                                                                                                                                                 tgGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGCCAGCGGt  <  1:287600/149‑1 (MQ=255)
                                                                                                                                                 tgGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGCCAGCGGt  >  3:291619/1‑149 (MQ=255)
                                                                                                                                                 tgGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGCCAGCGGt  >  5:171252/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTGTAGGAGCGGGCTTGCCCGCGAAGGGGCGCGAAGCGGCCCCAAAAATGGCGGATTGAAATACATGACACCCTGGCTGACAGTAGTAGGCATCGGCGAAGACGGCTTCAGCGGCCTGGGCAAACAGGCCCGGC‑‑GCGCCCTGCTGGGCGCTGGCCGGGTCTTCGGCAGCCCGCGCCAGCTGGCCTTGCTGCCACGTTGCGTGACCGCTGAACAGTTGGCCTGGCCCAGCCCGTTCTCGCTGGCCCCAGTGCTGGCCCTTCGCGGCGAGCCGGTGTGCGTGCTGGCCAGCGGT  >  NC_002947/5496864‑5497155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑