Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,576,118 A→G 7.7% F11L (TTC→CTC)  motA ← flagellar motor rotation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,576,1180AG 7.7% 147.6 / 2.6 52F11L (TTC→CTC) motAflagellar motor rotation protein
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base G (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGCCACTCAATGCGAAAAGCC  >  NC_002947/5575971‑5576259
                                                                                                                                                   |                                                                                                                                             
tcttGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGaa                                                                                                                                               >  1:77441/1‑148 (MQ=255)
tcttGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGaa                                                                                                                                               >  5:230251/1‑148 (MQ=255)
 cttGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGaa                                                                                                                                               <  6:334561/147‑1 (MQ=255)
  ttGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAGTac                                                                                                                                            >  1:14691/1‑149 (MQ=255)
  ttGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAGTac                                                                                                                                            >  3:137934/1‑149 (MQ=255)
   tgatgaCGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATacg                                                                                                                                           >  4:54571/1‑149 (MQ=255)
    gatgaCGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATa                                                                                                                                             <  2:158595/146‑1 (MQ=255)
     atgaCGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATacgac                                                                                                                                         >  6:218831/1‑149 (MQ=255)
      tgaCGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATacgacg                                                                                                                                        <  2:81470/149‑1 (MQ=255)
       gaCGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATAcgacga                                                                                                                                       >  6:107508/1‑149 (MQ=255)
         cGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACgat                                                                                                                                      >  5:278118/1‑148 (MQ=255)
         cGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACgat                                                                                                                                      >  2:33604/1‑148 (MQ=255)
            gCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGatgatg                                                                                                                                  >  4:58318/1‑149 (MQ=255)
             cATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGc                                                                                                                                 <  1:33604/149‑1 (MQ=255)
             cATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGc                                                                                                                                 <  5:218831/149‑1 (MQ=255)
                 gTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGa                                                                                                                              >  8:34462/1‑148 (MQ=255)
                    aTGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATaa                                                                                                                           <  6:230251/148‑1 (MQ=255)
                    aTGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAAt                                                                                                                          >  6:30368/1‑149 (MQ=255)
                                       gCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAg                                                                                                       >  3:319614/1‑149 (MQ=255)
                                              tGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGc                                                                                                <  4:319614/149‑1 (MQ=255)
                                                 aCCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGt                                                                                             >  1:9899/1‑149 (MQ=255)
                                                   cAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCg                                                                                           >  4:97045/1‑149 (MQ=255)
                                                      aGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAGTACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTgg                                                                                        <  2:14691/149‑1 (MQ=255)
                                                      aGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAGTACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTgg                                                                                        <  4:137934/149‑1 (MQ=255)
                                                        gCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGt                                                                                       >  2:177630/1‑148 (MQ=255)
                                                          cGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAg                                                                                    <  5:107508/149‑1 (MQ=255)
                                                               cgccgATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTaa                                                                               <  6:278118/149‑1 (MQ=255)
                                                                gccgATGATAAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCAGTGGGATAGCACGTAGCCGCCGAGCACGGTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAag                                                                              <  3:246462/149‑1 (MQ=255)
                                                                 ccgATGATCAGTACTTCGAACGGCTGGATCAGCGCCGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATAGGACGATGATGACGATAATTTTAGCCATAGGATCAAAGCACTAGCTGTCGTGGTCAggcaaa                                                                               >  4:72596/1‑143 (MQ=255)
                                                                  cgATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTaa                                                                               >  3:70317/1‑146 (MQ=255)
                                                                        tCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGt                                                                                       <  6:266647/132‑1 (MQ=255)
                                                                        tCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGt                                                                                       >  5:266647/1‑132 (MQ=255)
                                                                        tCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACgg                                                                        >  7:187440/1‑147 (MQ=255)
                                                                        tCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGa                                                                      <  7:34462/149‑1 (MQ=255)
                                                                           gTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCt                                                                   >  5:75848/1‑149 (MQ=255)
                                                                              cTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTa                                                                <  2:237904/149‑1 (MQ=255)
                                                                                   aaCGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAAct                                                           >  2:244036/1‑149 (MQ=255)
                                                                                     cGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAActct                                                         <  1:177630/149‑1 (MQ=255)
                                                                                         tGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACtcttctt                                                     >  5:155615/1‑149 (MQ=255)
                                                                                           gATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACtcttcttc                                                    <  3:54571/148‑1 (MQ=255)
                                                                                            aTCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACtcttcttct                                                   >  4:186478/1‑148 (MQ=255)
                                                                                             tCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTAc                                                 <  5:30368/149‑1 (MQ=255)
                                                                                             tCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTAc                                                 <  3:97045/149‑1 (MQ=255)
                                                                                                 cgcTGCGATCTTGCCGTGTGAGATCACGTAGCCTCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTAt                                             <  3:228008/149‑1 (MQ=255)
                                                                                                   cTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCg                                           <  4:70317/149‑1 (MQ=255)
                                                                                                      cGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGc                                         <  2:9899/148‑1 (MQ=255)
                                                                                                              ccGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAg                                                                                    >  6:330481/1‑97 (MQ=255)
                                                                                                              ccGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAg                                                                                    <  5:330481/97‑1 (MQ=255)
                                                                                                                        gCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACtata                      <  3:58318/149‑1 (MQ=255)
                                                                                                                         gaagTAGCCGCCGAGCACGCTCTCGAATACGACGATGATGCCTATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAg                     <  1:205942/146‑1 (MQ=255)
                                                                                                                          aCGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGc                    <  2:77441/149‑1 (MQ=255)
                                                                                                                            gTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGc                    <  7:112693/147‑1 (MQ=255)
                                                                                                                            gTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGc                    >  8:112693/1‑147 (MQ=255)
                                                                                                                                           gCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGCCACTCAATGCGAAAAGc   >  4:130913/1‑149 (MQ=255)
                                                                                                                                            cTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGCCACTCAATGCGAAAAGcc  <  8:187440/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TCTTGATGACGTGCATGGTCATGTGGCCAGGGTTGGCCTGCAGGAATGCACCAAAGGCCGCACCGCCGATGATCAGTACTTCGAACGGCTGGATCAGCGCTGCGATCTTGCCGTGGGAGAGCACGTAGCCGCCGAGCACGCTCGCGAATACGACGATGATGCCGATAATTTTAGCCATAGGTTCAAAGCACTTGCTGTCGTGGTCAGGGTAAGAGACGGGAGCTTTAAAACTCTTCTTCTACTTATCGGCAGAACTGCGCCAGACTATAGCCACTCAATGCGAAAAGCC  >  NC_002947/5575971‑5576259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑