Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,670,443	T→A	7.7%	intergenic (+32/‑53)	metF → / → PP_4978	5,10‑methylenetetrahydrofolate reductase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,670,443	0	T	A	7.7%	145.2 / 3.1	52	intergenic (+32/‑53)	metF/PP_4978	5,10‑methylenetetrahydrofolate reductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

CCGCATGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATC  >  NC_002947/5670300‑5670591                                                                                                                                                |                                                                                                                                                     ccGCATGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTaa                                                                                                                                                   >  1:150556/1‑147 (MQ=255) ccGCATGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTaa                                                                                                                                                   <  2:150556/147‑1 (MQ=255) ccGCATGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAgg                                                                                                                                                 >  7:39325/1‑149 (MQ=255)                  tGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTa                                                                                                                                 >  2:295606/1‑148 (MQ=255)                       cAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCt                                                                                                                           >  1:83802/1‑149 (MQ=255)                       cAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTAGTCTATGACCAAAGcc                                                                                                                            <  6:148813/148‑1 (MQ=255)                        aaGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCt                                                                                                                           >  7:198058/1‑148 (MQ=255)                         aGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTa                                                                                                                         <  4:321353/149‑1 (MQ=255)                          ggcggcGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTAt                                                                                                                        <  2:287116/149‑1 (MQ=255)                               cgcgcCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCAtt                                                                                                                   <  4:245239/149‑1 (MQ=255)                                gcgcCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCAttt                                                                                                                  <  6:308947/149‑1 (MQ=255)                                   ccAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCg                                                                                                               >  8:177010/1‑149 (MQ=255)                                    cAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCgg                                                                                                              <  8:198058/149‑1 (MQ=255)                                    cAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCgg                                                                                                              <  5:76239/149‑1 (MQ=255)                                     aGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCggg                                                                                                             >  3:177565/1‑149 (MQ=255)                                      ggCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaag                                                                                                            <  8:229866/149‑7 (MQ=37)                                            cACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTaaa                                                                                                        <  1:295606/147‑1 (MQ=255)                                             aCTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAg                                                                                                     <  3:35677/149‑1 (MQ=255)                                             aCTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAg                                                                                                     <  5:163644/149‑1 (MQ=255)                                              cTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAGGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAgg                                                                                                    >  4:245909/1‑149 (MQ=255)                                               ttCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGt                                                                                                   <  2:83802/149‑1 (MQ=255)                                                  tataCCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAttt                                                                                                                  <  5:244394/131‑1 (MQ=37)                                                    taCCTTCAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCCCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTagc                                                                                              <  8:242132/149‑1 (MQ=255)                                                       cTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgcct                                                                                            >  5:320636/1‑148 (MQ=255)                                                        ttGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgcctg                                                                                           >  6:330654/1‑148 (MQ=255)                                                             ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATg                                                                                      >  8:41277/1‑148 (MQ=255)                                                             ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaa                                                                                                             >  7:107023/1‑120 (MQ=25)                                                             ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaa                                                                                                             <  8:107023/125‑6 (MQ=25)                                                                 gCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATgcagca                                                                                 >  8:100074/1‑149 (MQ=255)                                                                   tGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGcaca                                                                               >  1:147277/1‑149 (MQ=255)                                                                        cGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCaa                                                                          <  7:41277/149‑1 (MQ=255)                                                                                gCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTcgcg                                                                  >  5:265977/1‑149 (MQ=255)                                                                                gCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTcgcg                                                                  <  6:265977/149‑1 (MQ=255)                                                                                           caacCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTGATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACCGCCAACCTTCGCGCCCACAGCTTg                                                       >  8:107628/1‑149 (MQ=255)                                                                                               cTGAAGCTGCCGCCCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTCAAACAGGTCTTGCATGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGtat                                                    >  6:28236/1‑148 (MQ=255)                                                                                                    gCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGGCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTgg                                              >  3:88686/1‑149 (MQ=255)                                                                                                         cgcgCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCttt                                          <  5:330654/148‑1 (MQ=255)                                                                                                             cTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGGCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGcc                                     <  4:88686/149‑1 (MQ=255)                                                                                                             cTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGcc                                     >  6:125784/1‑149 (MQ=255)                                                                                                              tGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGccc                                    >  4:4013/1‑149 (MQ=255)                                                                                                               gAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCg                                   >  3:68318/1‑149 (MQ=255)                                                                                                                aaaTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCg                                   <  1:237390/148‑1 (MQ=255)                                                                                                                 aaTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCgg                                  >  1:124425/1‑148 (MQ=255)                                                                                                                        tGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTa                           <  3:4013/148‑1 (MQ=255)                                                                                                                            aaaGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCAt                       >  5:172651/1‑148 (MQ=255)                                                                                                                                    cTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGaa                 <  4:177565/146‑1 (MQ=255)                                                                                                                                    cTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGc               <  2:147277/148‑1 (MQ=255)                                                                                                                                     ttAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCt             >  3:2440/1‑149 (MQ=255)                                                                                                                                       cgaaaaTGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGc           >  7:285094/4‑149 (MQ=37)                                                                                                                                           aaTGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTc        <  3:245909/148‑1 (MQ=255)                                                                                                                                           aaTGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCa       <  6:320636/149‑1 (MQ=255)                                                                                                                                            aTGATTAAGGGTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATAGCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCa       <  7:100074/148‑1 (MQ=255)                                                                                                                                                ttAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc  <  6:95847/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CCGCATGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATC  >  NC_002947/5670300‑5670591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑