Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,670,473 T→A 10.0% intergenic (+62/‑23) metF → / → PP_4978 5,10‑methylenetetrahydrofolate reductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,670,4730TA10.0% 153.7 / 8.4 60intergenic (+62/‑23)metF/PP_49785,10‑methylenetetrahydrofolate reductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base A (2/4);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

GGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGGCAGCGCA  >  NC_002947/5670325‑5670621
                                                                                                                                                    |                                                                                                                                                    
ggcggcGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTAt                                                                                                                                                      <  2:287116/149‑1 (MQ=255)
     cgcgcCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCAtt                                                                                                                                                 <  4:245239/149‑1 (MQ=255)
      gcgcCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCAttt                                                                                                                                                <  6:308947/149‑1 (MQ=255)
         ccAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCg                                                                                                                                             >  8:177010/1‑149 (MQ=255)
          cAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCgg                                                                                                                                            <  8:198058/149‑1 (MQ=255)
          cAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCgg                                                                                                                                            <  5:76239/149‑1 (MQ=255)
           aGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCggg                                                                                                                                           >  3:177565/1‑149 (MQ=255)
            ggCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaag                                                                                                                                          <  8:229866/149‑7 (MQ=37)
                  cACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTaaa                                                                                                                                      <  1:295606/147‑1 (MQ=255)
                   aCTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAg                                                                                                                                   <  3:35677/149‑1 (MQ=255)
                   aCTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAg                                                                                                                                   <  5:163644/149‑1 (MQ=255)
                    cTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAGGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAgg                                                                                                                                  >  4:245909/1‑149 (MQ=255)
                     ttCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGt                                                                                                                                 <  2:83802/149‑1 (MQ=255)
                        tataCCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAttt                                                                                                                                                <  5:244394/131‑1 (MQ=37)
                          taCCTTCAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCCCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTagc                                                                                                                            <  8:242132/149‑1 (MQ=255)
                             cTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgcct                                                                                                                          >  5:320636/1‑148 (MQ=255)
                              ttGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgcctg                                                                                                                         >  6:330654/1‑148 (MQ=255)
                                   ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATg                                                                                                                    >  8:41277/1‑148 (MQ=255)
                                   ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaa                                                                                                                                           <  8:107023/125‑6 (MQ=25)
                                   ccAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaa                                                                                                                                           >  7:107023/1‑120 (MQ=25)
                                       gCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATgcagca                                                                                                               >  8:100074/1‑149 (MQ=255)
                                         tGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGcaca                                                                                                             >  1:147277/1‑149 (MQ=255)
                                              cGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCaa                                                                                                        <  7:41277/149‑1 (MQ=255)
                                                      gCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTcgcg                                                                                                <  6:265977/149‑1 (MQ=255)
                                                      gCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTcgcg                                                                                                >  5:265977/1‑149 (MQ=255)
                                                                 caacCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTGATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACCGCCAACCTTCGCGCCCACAGCTTg                                                                                     >  8:107628/1‑149 (MQ=255)
                                                                     cTGAAGCTGCCGCCCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTCAAACAGGTCTTGCATGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGtat                                                                                  >  6:28236/1‑148 (MQ=255)
                                                                          gCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGGCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTgg                                                                            >  3:88686/1‑149 (MQ=255)
                                                                               cgcgCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCttt                                                                        <  5:330654/148‑1 (MQ=255)
                                                                                   cTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGGCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGcc                                                                   <  4:88686/149‑1 (MQ=255)
                                                                                   cTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGcc                                                                   >  6:125784/1‑149 (MQ=255)
                                                                                    tGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGccc                                                                  >  4:4013/1‑149 (MQ=255)
                                                                                     gAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCg                                                                 >  3:68318/1‑149 (MQ=255)
                                                                                      aaaTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCg                                                                 <  1:237390/148‑1 (MQ=255)
                                                                                       aaTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCgg                                                                >  1:124425/1‑148 (MQ=255)
                                                                                              tGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTa                                                         <  3:4013/148‑1 (MQ=255)
                                                                                                  aaaGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCAt                                                     >  5:172651/1‑148 (MQ=255)
                                                                                                          cTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGaa                                               <  4:177565/146‑1 (MQ=255)
                                                                                                          cTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGc                                             <  2:147277/148‑1 (MQ=255)
                                                                                                           ttAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCt                                           >  3:2440/1‑149 (MQ=255)
                                                                                                             cgaaaaTGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGc                                         >  7:285094/4‑149 (MQ=37)
                                                                                                                 aaTGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTc                                      <  3:245909/148‑1 (MQ=255)
                                                                                                                 aaTGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCa                                     <  6:320636/149‑1 (MQ=255)
                                                                                                                  aTGATTAAGGGTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATAGCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCa                                     <  7:100074/148‑1 (MQ=255)
                                                                                                                      ttAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc                                <  6:95847/149‑1 (MQ=255)
                                                                                                                       tAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCg                               >  7:178992/1‑149 (MQ=255)
                                                                                                                          ggCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGt                              >  5:191823/1‑147 (MQ=255)
                                                                                                                            cTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAgc                          <  5:125784/149‑1 (MQ=255)
                                                                                                                               tGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAgcg                         <  4:2440/147‑1 (MQ=255)
                                                                                                                                  tCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACACGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTc                    >  1:60820/1‑149 (MQ=255)
                                                                                                                                     tAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGcc                 >  1:71597/1‑149 (MQ=255)
                                                                                                                                         cTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGc             <  6:172651/149‑1 (MQ=255)
                                                                                                                                         cTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGc             <  7:177010/149‑1 (MQ=255)
                                                                                                                                          tAAAGCCTTATTCATTCTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGc             >  3:103939/1‑148 (MQ=255)
                                                                                                                                          caaaGCCTTAATCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCt            <  8:285094/148‑1 (MQ=255)
                                                                                                                                              gCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc                                >  1:241851/1‑125 (MQ=255)
                                                                                                                                              gCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc                                <  2:241851/125‑1 (MQ=255)
                                                                                                                                               ccTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAgg         >  6:85103/1‑147 (MQ=255)
                                                                                                                                                 ttATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCACGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGgcagc     >  5:271130/1‑149 (MQ=255)
                                                                                                                                                  tATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGgcagcg    >  1:328427/1‑149 (MQ=255)
                                                                                                                                                   aTTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGgcagc     <  8:39325/147‑1 (MQ=255)
                                                                                                                                                    ttCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGgcagcgca  <  2:124425/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGTCAGCGCCCTCGCCCAGCTAGGCAGCGCA  >  NC_002947/5670325‑5670621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑