Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,674,753 G→C 100% intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7530GC100.0% 139.3 / NA 41intergenic (+115/+25)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (26/15);  total (26/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA  >  NC_002947/5674613‑5674892
                                                                                                                                              |                                                                                                                                           
cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCgg                                                                                                                                      <  2:82844/149‑1 (MQ=255)
           aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAACGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTAGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAgtta                                                                                                                            >  7:48560/1‑145 (MQ=255)
                                        gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                              <  6:304981/149‑1 (MQ=255)
                                               ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                        >  8:192648/1‑148 (MQ=255)
                                                   cAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                    >  4:306074/1‑148 (MQ=255)
                                                       ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                               >  6:60196/1‑149 (MQ=255)
                                                         tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                    <  6:344138/110‑1 (MQ=255)
                                                         tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                    >  5:344138/1‑110 (MQ=255)
                                                              cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                         >  6:242356/1‑148 (MQ=255)
                                                                 cacaACGCAGCTCCAGCAGAGAATTTCTTGATATCTACAGGATCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCCGTTTTCAGCTGGCAGAAATTACTTCTGATTCACACCTTCGACACTGAAATCCAGGACCAGGGTCTg                                                                     >  8:7545/1‑149 (MQ=255)
                                                                         cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGTTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                             <  5:242356/149‑1 (MQ=255)
                                                                         cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                             >  1:95270/1‑149 (MQ=255)
                                                                              cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAccc                                                        >  6:116985/1‑149 (MQ=255)
                                                                               tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                          >  4:174545/1‑130 (MQ=255)
                                                                               tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                          <  3:174545/130‑1 (MQ=255)
                                                                                   gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGcc                                                   >  2:149252/1‑149 (MQ=255)
                                                                                             aattctcTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTC‑GTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGa                                        <  8:124287/146‑1 (MQ=38)
                                                                                                tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTTGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGCCACTGCGAAGTAGCACCCAGGCCTTTGATGCAGAAg                                      >  4:254663/1‑149 (MQ=38)
                                                                                                tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAAAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                      >  3:56179/1‑149 (MQ=255)
                                                                                                  tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                                 >  1:293104/1‑120 (MQ=255)
                                                                                                  tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                                 <  2:293104/120‑1 (MQ=255)
                                                                                                   ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACCCCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCATTGATGCCGAAGTCg                                   >  4:12050/1‑149 (MQ=39)
                                                                                                    tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                       <  2:95270/144‑1 (MQ=255)
                                                                                                     aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt                                  >  8:238174/1‑148 (MQ=255)
                                                                                                      cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttc                                <  5:60196/149‑1 (MQ=255)
                                                                                                       aGGAGCTGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCTGTTTTCATCTGGCAGAAATTACTTCTGCTTCACACCTTCGAGACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttc                                <  3:254663/148‑1 (MQ=255)
                                                                                                           gCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                           >  6:213678/1‑149 (MQ=255)
                                                                                                              gCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttcag                        >  7:306961/1‑149 (MQ=255)
                                                                                                               ccTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                       <  5:116985/149‑1 (MQ=255)
                                                                                                                      tCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg                <  3:306074/149‑1 (MQ=255)
                                                                                                                        gTGATAGGGCCGCAAAGGG‑GCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTGACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGGACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg              <  7:7545/149‑1 (MQ=255)
                                                                                                                        gTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg              >  8:116412/1‑149 (MQ=255)
                                                                                                                         tGATAGGGCCACAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAATGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg             >  2:160544/1‑149 (MQ=255)
                                                                                                                              gggCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAAAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt         <  4:56179/148‑1 (MQ=255)
                                                                                                                               ggCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg        >  2:117396/1‑148 (MQ=255)
                                                                                                                                gCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg        >  6:182084/1‑147 (MQ=255)
                                                                                                                                gCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg        >  4:113526/1‑147 (MQ=255)
                                                                                                                                 ccGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg     >  4:6839/1‑149 (MQ=255)
                                                                                                                                 ccGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGAGGTGGTGGCGTTGAAg     >  8:250826/1‑149 (MQ=255)
                                                                                                                                  cGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc    >  3:91017/1‑149 (MQ=255)
                                                                                                                                    cAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCa  <  5:213678/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCA  >  NC_002947/5674613‑5674892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑