Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,705,755 A→G 5.1% L16L (CTT→CTC)  PP_5008 ← poly granule‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,705,7550AG 5.1% 238.8 / 2.3 78L16L (CTT→CTC) PP_5008poly granule‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (36/38);  new base G (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GTCGATGCGCTTCTTGCCGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAACCTCACCCTAAGGTC  >  NC_002947/5705611‑5705901
                                                                                                                                                |                                                                                                                                                  
gTCGATGCGCTTCTTGCCGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGc                                                                                                                                                 <  7:204679/148‑1 (MQ=255)
  cGATGCGCTTCTTGCCGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCgtg                                                                                                                                              >  1:274581/1‑149 (MQ=255)
   gATGCGCTTCTTGCCGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCgtgt                                                                                                                                             >  7:7610/1‑149 (MQ=255)
                ccGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGt                                                                                                                                 <  8:7610/148‑1 (MQ=255)
                ccGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTc                                                                                                                                >  6:37647/1‑149 (MQ=255)
                ccGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTc                                                                                                                                >  7:253861/1‑149 (MQ=255)
                 cgcgCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCt                                                                                                                               <  2:274581/149‑1 (MQ=255)
                    gcTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGTCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCtttt                                                                                                                            <  1:134786/149‑1 (MQ=255)
                    gcTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCtttt                                                                                                                            <  2:111244/149‑1 (MQ=255)
                      ttCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCttttt                                                                                                                           >  3:97948/1‑148 (MQ=255)
                        ctcGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCtttttt                                                                                                                          <  3:175381/147‑1 (MQ=255)
                        ctcGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCtttttt                                                                                                                          <  3:104512/147‑1 (MQ=255)
                        ctcGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTttc                                                                                                                         <  3:270101/148‑1 (MQ=255)
                         tagACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCGAGCGTGTCCAGGGCTTCGTCTTTTttctt                                                                                                                       >  6:170187/3‑149 (MQ=255)
                         tagACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCGAGCGTGTCCAGGGCTTCGTCTTTTttctt                                                                                                                       >  6:165384/3‑149 (MQ=255)
                                cTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAAt                                                                                                                 >  7:289793/1‑148 (MQ=255)
                                cTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAAt                                                                                                                 >  5:98883/1‑148 (MQ=255)
                                 ttCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCa                                                                                                               >  7:219359/1‑149 (MQ=255)
                                    aCCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACtt                                                                                                            <  1:187070/149‑1 (MQ=255)
                                      ccGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTg                                                                                                          >  4:287127/1‑149 (MQ=255)
                                      ccGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTg                                                                                                          <  7:302976/149‑1 (MQ=255)
                                      ccGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTg                                                                                                          >  1:237617/1‑149 (MQ=255)
                                      ccGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGATTCGTCTTTTTTCTTCGCAATCACTTTg                                                                                                          >  8:132989/1‑149 (MQ=255)
                                         cccTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCCTTTTTCTTCGCAATCACTTTGGcc                                                                                                       <  5:37647/149‑1 (MQ=255)
                                          ccTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCa                                                                                                      <  7:183075/149‑1 (MQ=255)
                                              aCCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCt                                                                                                  <  2:237617/149‑1 (MQ=255)
                                                cAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCtgt                                                                                                <  8:253861/149‑1 (MQ=255)
                                                   ctctTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTa                                                                                             >  7:221081/1‑149 (MQ=255)
                                                   ctctTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTa                                                                                             >  8:136975/1‑149 (MQ=255)
                                                      tttGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGc                                                                                                                    <  6:182484/123‑1 (MQ=255)
                                                      tttGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACct                                                                                          >  4:222889/1‑149 (MQ=255)
                                                         gAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACctca                                                                                        <  7:180615/148‑1 (MQ=255)
                                                                tCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTAc                                                                                            >  6:29807/1‑137 (MQ=255)
                                                                tCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTAc                                                                                            <  5:29807/137‑1 (MQ=255)
                                                                  aGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAg                                                                              <  7:132989/149‑1 (MQ=255)
                                                                   gcgcCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgt                                                                             >  2:239416/1‑149 (MQ=255)
                                                                    cgcCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtg                                                                            <  6:117051/149‑1 (MQ=255)
                                                                     gcCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtgg                                                                           <  8:288687/149‑1 (MQ=255)
                                                                     gcCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtgg                                                                           >  5:336724/1‑149 (MQ=255)
                                                                      ccTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtggt                                                                          <  7:249758/149‑1 (MQ=255)
                                                                       cTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCGAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtggtg                                                                         <  5:170187/149‑1 (MQ=255)
                                                                       cTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCGAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtggtg                                                                         <  5:165384/149‑1 (MQ=255)
                                                                        ttCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtggtg                                                                         >  4:191733/1‑148 (MQ=255)
                                                                        ttCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAgtggtg                                                                         >  7:15091/1‑148 (MQ=255)
                                                                           cTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGaa                                                                      >  8:180869/1‑148 (MQ=255)
                                                                           cTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGaa                                                                      >  8:176070/1‑148 (MQ=255)
                                                                            tGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGaaaa                                                                    >  3:116652/1‑149 (MQ=255)
                                                                            tGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGATCATGAGTGTTGGaaaa                                                                    >  4:210013/1‑149 (MQ=255)
                                                                                 gACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGcc                                                               <  4:116652/149‑1 (MQ=255)
                                                                                 gACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGcc                                                               >  2:314526/1‑149 (MQ=255)
                                                                                  aCGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGccc                                                              <  8:15091/149‑1 (MQ=255)
                                                                                      gggCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATgg                                                          <  8:221081/149‑1 (MQ=255)
                                                                                          gTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCGGTGTACCTCATGCTGATGAGTGGTGGAAAACCGCCCATGGAGGc                                                      >  5:189713/1‑149 (MQ=255)
                                                                                                   gATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGa                                             <  1:239416/149‑1 (MQ=255)
                                                                                                    aTACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAg                                            <  3:287127/149‑1 (MQ=255)
                                                                                                     tACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGc                                           <  3:191733/149‑1 (MQ=255)
                                                                                                      aCCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCa                                          >  7:316461/1‑149 (MQ=255)
                                                                                                        cGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACt                                                                                                             >  8:67735/1‑80 (MQ=255)
                                                                                                         ggCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCAtt                                        >  4:243880/1‑148 (MQ=255)
                                                                                                         ggCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTa                                       >  7:137028/1‑149 (MQ=255)
                                                                                                          gccagccaGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTaa                                      <  1:314526/149‑1 (MQ=255)
                                                                                                           ccagccagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAg                                     <  7:180869/149‑1 (MQ=255)
                                                                                                           ccagccagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGCCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAg                                     <  7:176070/149‑1 (MQ=255)
                                                                                                              gccagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAgg                                    >  1:261893/1‑147 (MQ=255)
                                                                                                               ccagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGa                                             >  2:335684/1‑137 (MQ=255)
                                                                                                               ccagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGa                                             <  1:335684/137‑1 (MQ=255)
                                                                                                               ccagATCTTGCGCGCATAGCAGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGATAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAg                                 <  5:110893/149‑1 (MQ=255)
                                                                                                                cagATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAgg                                >  1:178826/1‑149 (MQ=255)
                                                                                                                      ttGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGgaaga                           >  6:295338/1‑148 (MQ=255)
                                                                                                                        gcgcgcATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGaaaa                        >  3:213619/1‑149 (MQ=255)
                                                                                                                        gcgcgcATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGaaa                         <  7:136975/148‑1 (MQ=255)
                                                                                                                        gcgcgcATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGaaa                         <  6:336724/148‑1 (MQ=255)
                                                                                                                           cgcATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTa                     <  4:97948/149‑1 (MQ=255)
                                                                                                                            ggattgCCGGGCACCTCGCCAAGCGTGTCCAGGGCTTCGTGTTTTTTCTTCGCTATCACTTTGGCCTTGCTGTGTACCTCATGCTCATGAGTGGTGTAAAACGGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAAt                       <  3:210013/141‑1 (MQ=255)
                                                                                                                             cATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTATTCATCCCAATCACTTTGGCCATGCTGTGTACCTCATGCACATCAGTGGTGGAAAACCGCCCATGCAGGCTGGGCTTGAGCATTAAGGTAGGGATGAAAATTAg                    >  2:147990/1‑148 (MQ=255)
                                                                                                                                aGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAAc                <  2:261893/149‑1 (MQ=255)
                                                                                                                                          cTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAACCTCACCCTaa      >  4:114582/1‑149 (MQ=255)
                                                                                                                                              ccAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAACCTCACCCTAAGgtc  <  2:178826/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GTCGATGCGCTTCTTGCCGCGCTTCTCGACACCTTCACCCGCCCTGACCAGCTCTTTGAAGTAGTCAGCGCCTTCCTGACCGACGCGGGCGTAGGCGCCGATACCGGCCAGCCAGATCTTGCGCGCATAGCCGCGCACCTCGCCAAGCGTGTCCAGGGCTTCGTCTTTTTTCTTCGCAATCACTTTGGCCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAACCTCACCCTAAGGTC  >  NC_002947/5705611‑5705901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑