Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,728,840 A→G 6.6% intergenic (‑203/+121) opgG ← / ← dtd glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,728,8400AG 6.6% 222.6 / 4.6 76intergenic (‑203/+121)opgG/dtdglucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base G (3/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AATCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGG  >  NC_002947/5728692‑5728988
                                                                                                                                                    |                                                                                                                                                    
aaTCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTa                                                                                                                                                      <  3:80970/149‑1 (MQ=255)
  tCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTAc                                                                                                                                                     >  4:290798/1‑148 (MQ=255)
    aCGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGaa                                                                                                                                                  >  3:194694/1‑149 (MQ=255)
      gcgCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACACCGAGAGGGCGTAATGATTCCCCACACGGATCCAGACACTGCTCGAGTCAGCGTGAAACGGACCTACGAACg                                                                                                                                                >  7:20991/3‑149 (MQ=255)
      gAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCACCAAACGGATCCAGACAATGATCGAGTCAGAGTGAAACGGACCTACGAACg                                                                                                                                                >  7:26029/1‑149 (MQ=255)
       aGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGc                                                                                                                                               <  7:43342/149‑1 (MQ=255)
        gCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACg                                                                                                                                                >  1:210564/1‑147 (MQ=255)
            cccTCGCCGAAACCAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACGCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTc                                                                                                                                          <  5:107632/149‑1 (MQ=255)
            cccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTc                                                                                                                                          <  3:289244/149‑1 (MQ=255)
             ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTcc                                                                                                                                         <  4:105615/149‑1 (MQ=255)
             ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTcc                                                                                                                                         <  6:191326/149‑1 (MQ=255)
                          aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                            <  4:149465/149‑1 (MQ=255)
                          aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCACCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                            >  2:124440/1‑149 (MQ=255)
                           gATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGAt                                                                                                                           <  4:194694/149‑1 (MQ=255)
                              cATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTAt                                                                                                                        >  3:143875/1‑149 (MQ=255)
                               aTGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTAAGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                                       <  1:30302/149‑1 (MQ=255)
                                  aaaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCca                                                                                                                     <  4:29940/148‑1 (MQ=255)
                                    acgaaCGGACTGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcagca                                                                                                                  <  6:343826/146‑1 (MQ=255)
                                    aCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcag                                                                                                                    >  8:234761/1‑147 (MQ=255)
                                            ccAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGt                                                                                                          <  3:317781/149‑1 (MQ=255)
                                              aGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGa                                                                                                        >  8:159807/1‑149 (MQ=255)
                                                    aaaTGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACg                                                                                                   >  5:192341/1‑148 (MQ=255)
                                                       tGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCAt                                                                                                >  2:113316/1‑148 (MQ=255)
                                                                 gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAg                                                                                      >  6:331518/1‑148 (MQ=255)
                                                                  cGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCCTGATGCGTTGCCACGCAACCTGCTGGataa                                                                                    <  5:134747/149‑4 (MQ=255)
                                                                   gAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCaa                                                                                   <  5:331518/149‑1 (MQ=255)
                                                                    atcACTGTTCGGACCCCGAGAGGGCTGAATTATTCCAAAAACGGATCGAGACAATGCTCGAGTCTGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCTTGACAACGCATCATTCTGGAGCaaa                                                                                  <  8:227925/147‑2 (MQ=255)
                                                                       aCTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAt                                                                               >  8:261899/1‑149 (MQ=255)
                                                                         tGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAtt                                                                              >  5:37858/1‑148 (MQ=255)
                                                                           ttCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTa                                                                            <  1:124440/148‑1 (MQ=255)
                                                                              ggACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                         <  3:147494/148‑1 (MQ=255)
                                                                              ggACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                         >  4:271614/1‑148 (MQ=255)
                                                                              ggACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                         <  3:290798/148‑1 (MQ=255)
                                                                                  cccGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAgg                                                                                            >  8:126216/1‑125 (MQ=255)
                                                                                  cccGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAgg                                                                                            <  7:126216/125‑1 (MQ=255)
                                                                                  cccGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACa                                                                     >  2:285150/1‑148 (MQ=255)
                                                                                     gagaGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCTACTAt                                                                               >  6:2080/1‑135 (MQ=255)
                                                                                     gagaGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAt                                                                               <  5:2080/135‑1 (MQ=255)
                                                                                     gagaGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAAc                                                                 <  7:159807/149‑1 (MQ=255)
                                                                                      agagGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAAcc                                                                <  6:37858/149‑1 (MQ=255)
                                                                                          ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCa                                                                                                                                    <  4:46009/77‑1 (MQ=255)
                                                                                          ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCa                                                                                                                                    >  3:46009/1‑77 (MQ=255)
                                                                                            cGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGt                                                          <  2:210564/149‑1 (MQ=255)
                                                                                               aaTGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                            <  2:179718/144‑1 (MQ=255)
                                                                                               aaTGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                            >  1:179718/1‑144 (MQ=255)
                                                                                                      ccccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                            >  6:154908/1‑137 (MQ=255)
                                                                                                      ccccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                            <  5:154908/137‑1 (MQ=255)
                                                                                                          aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaa                                            >  8:101581/1‑149 (MQ=255)
                                                                                                           aaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaac                                           >  4:106747/1‑149 (MQ=255)
                                                                                                             cGGATACAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCTATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACgg                                         <  7:181891/149‑1 (MQ=255)
                                                                                                                   cAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGttttt                                   >  7:186291/1‑149 (MQ=255)
                                                                                                                    aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                    <  5:146998/131‑1 (MQ=255)
                                                                                                                    aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                    >  6:146998/1‑131 (MQ=255)
                                                                                                                    aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                  >  3:275368/1‑149 (MQ=255)
                                                                                                                    aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                  <  3:106747/149‑1 (MQ=255)
                                                                                                                    aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                  <  1:285150/149‑1 (MQ=255)
                                                                                                                      aCAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGa                                <  4:143875/149‑1 (MQ=255)
                                                                                                                      aCAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGa                                <  3:271614/149‑1 (MQ=255)
                                                                                                                       cAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGAc                               <  3:312483/149‑1 (MQ=255)
                                                                                                                        aaTGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCaa                                                                                   >  8:109767/1‑96 (MQ=255)
                                                                                                                        aaTGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGAcc                              >  2:36654/1‑149 (MQ=255)
                                                                                                                            cTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCa                           <  1:113316/148‑1 (MQ=255)
                                                                                                                             tCGAGTCAGAGTGAAACGGACCTACGAACGCAAGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCata                         >  1:212547/1‑149 (MQ=255)
                                                                                                                                aGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAttt                      >  8:195997/1‑149 (MQ=255)
                                                                                                                                 gTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTg                     >  3:276326/1‑149 (MQ=255)
                                                                                                                                  tCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTg                     >  6:33205/1‑148 (MQ=255)
                                                                                                                                     gagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAAc                 <  7:234761/149‑1 (MQ=255)
                                                                                                                                      agTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                    >  3:24338/1‑113 (MQ=255)
                                                                                                                                      agTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                    <  4:24338/113‑1 (MQ=255)
                                                                                                                                       gTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACAt               >  4:3530/1‑149 (MQ=255)
                                                                                                                                       gTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACAt               <  8:186291/149‑1 (MQ=255)
                                                                                                                                           catggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt           >  8:250919/4‑149 (MQ=255)
                                                                                                                                           catggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCACTTGTTTTCCTGTGAACAACGGGTTTTTTTAACTCATATTTTAAACTTAAAt           >  1:303052/4‑149 (MQ=255)
                                                                                                                                           aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt           >  3:195273/1‑149 (MQ=255)
                                                                                                                                           aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACACCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt           >  7:111480/1‑149 (MQ=255)
                                                                                                                                            atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAC‑AAACCCCTTg                                                           >  6:129544/3‑99 (MQ=255)
                                                                                                                                            atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAC‑AAACCCCTTg                                                           <  5:129544/97‑1 (MQ=255)
                                                                                                                                                    aCGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCggt  >  5:8196/1‑148 (MQ=255)
                                                                                                                                                    aCGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCggg  >  4:167647/1‑149 (MQ=255)
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AATCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGG  >  NC_002947/5728692‑5728988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑