Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,365	A→G	8.9%	intergenic (‑198/+49)	fbp ← / ← glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,365	0	A	G	8.9%	155.8 / 5.3	56	intergenic (‑198/+49)	fbp/glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base G (2/3);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

GAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743220‑5743509                                                                                                                                                    |                                                                                                                                                 gagTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCt                                                                                                                                               >  2:84479/1‑149 (MQ=255)    tGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGc                                                                                                                                            <  8:176746/149‑1 (MQ=255)      aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCga                                                                                                                                          <  3:192747/149‑1 (MQ=255)      aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCga                                                                                                                                          <  4:56372/149‑1 (MQ=255)      aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCga                                                                                                                                          <  8:222923/149‑1 (MQ=255)      aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCg                                                                                                                                           <  7:250648/148‑1 (MQ=255)        gggCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCgaga                                                                                                                                        <  1:294796/149‑1 (MQ=255)            tAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtacc                                                                                                                                    >  7:112013/1‑142 (MQ=25)            tAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTGCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGCCCATTCGCTCGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGacac                                                                                                                                     <  1:84479/149‑1 (MQ=255)                gAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCtgtg                                                                                                                                <  5:255937/149‑1 (MQ=255)                gAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGT‑GTCTGGTTGTGTGTCCATTCGC‑GGGCACGCCC‑GCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTgg                                                                                                                               <  2:127323/149‑1 (MQ=18)                     atgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGc                                                                                                                           >  3:236987/1‑149 (MQ=255)                      tgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGATCTGGTTGTGTGTCCATTCGC‑GGGCACGCCC‑GCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTgg                                                                                                                               >  2:189265/1‑144 (MQ=18)                      tgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGATCTGGTTGTGTGTCCATTCGC‑GGGCACGCCC‑GCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTgg                                                                                                                               <  1:189265/144‑1 (MQ=18)                        agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCCGTGGGAGCga                                                                                                                         <  8:112013/148‑2 (MQ=21)                          aCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCg                                                                                                                      >  7:203493/1‑149 (MQ=255)                               ccACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGccc                                                                                                                 >  3:257986/1‑149 (MQ=255)                                 aCGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGGCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCcgc                                                                                                               <  7:301037/149‑1 (MQ=255)                                    gCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGAGAGACACCTGTGGGAGCGGGCGTACCcgaac                                                                                                             >  6:92021/1‑145 (MQ=255)                                    gCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCAATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTACCcgaac                                                                                                             >  6:96796/1‑145 (MQ=255)                                      tctcAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTAGAACCTTGCGAGACACCTGTGGGAGCGCGCGTGCCCGCGAACg                                                                                                          >  6:264262/1‑149 (MQ=255)                                          aGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcct                                                                                                      >  7:299883/1‑149 (MQ=255)                                            aCGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctca                                                                                                    >  3:147991/1‑149 (MQ=255)                                                             tAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCtt                                                                                   <  4:129546/149‑1 (MQ=255)                                                                ttCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTCGGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTc                                                                                  >  1:93614/1‑148 (MQ=255)                                                                 tCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCa                                                                                <  8:299883/148‑1 (MQ=255)                                                                       ttCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCg                                                                         >  7:161828/1‑149 (MQ=255)                                                                          gCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTg                                                                      <  2:93614/149‑1 (MQ=255)                                                                               gCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTAGTTGGCata                                                                 >  6:314382/1‑149 (MQ=255)                                                                                    tggtCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCAGAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGACATATTcgc                                                            <  4:236987/149‑1 (MQ=255)                                                                                         tGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCACCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATg                                                       >  1:35484/1‑149 (MQ=255)                                                                                           gttgttTGTCCTTTGGG‑TGGCGCGGTC‑GCTCCCACAGGTACAGCTCTAGCATTGAAGTTTGTGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATgg                                                      <  1:134678/148‑1 (MQ=255)                                                                                            ttGTGTCGCCCTTCGC‑GGGCGCGCTC‑GCTCCCACCGGTACAGCCCAAGCCTTGAAGCTTGCGAGACACCTGTTGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                                     <  4:257986/148‑1 (MQ=38)                                                                                                         cgc‑gGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACgag                                        <  2:35484/148‑1 (MQ=255)                                                                                                          gc‑gGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACgaga                                       <  8:203493/148‑1 (MQ=255)                                                                                                                cgcggTC‑GCTCCCAGAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCGGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGc                                 <  5:264262/149‑1 (MQ=255)                                                                                                                   gctc‑gctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCa                              >  3:27610/1‑149 (MQ=255)                                                                                                                     tc‑gctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATc                            >  6:52934/1‑149 (MQ=255)                                                                                                                      c‑gctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATcc                           <  5:1895/149‑1 (MQ=255)                                                                                                                        gctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATcc                           <  5:52934/148‑1 (MQ=255)                                                                                                                          tcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtg                        >  5:71571/1‑149 (MQ=255)                                                                                                                            ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGc                                 <  3:242290/138‑1 (MQ=255)                                                                                                                            ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGc                                 >  4:242290/1‑138 (MQ=255)                                                                                                                            ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgcc                      >  6:88914/1‑149 (MQ=255)                                                                                                                            ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgcc                      >  6:93694/1‑149 (MQ=255)                                                                                                                             cacaGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                                    <  2:66632/118‑1 (MQ=255)                                                                                                                                aGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCgtgt                  <  4:147991/149‑1 (MQ=255)                                                                                                                                 ggTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGtt                 <  4:27610/149‑1 (MQ=255)                                                                                                                                 ggTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGtt                 <  6:71571/149‑1 (MQ=255)                                                                                                                                  gTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGtt                 >  4:238041/1‑148 (MQ=255)                                                                                                                                           aaGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGcc       >  3:56665/1‑149 (MQ=255)                                                                                                                                             gCCTTGAATCTTGCTAGACACCTGTGGGAGCGGGCTTGCCCGCGAACTGCCTCACCTCAATCCAATGCCTTCCAGATCTCTGTGGCATATTCTCGAATGGTGCTGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCtt     <  5:314382/149‑1 (MQ=255)                                                                                                                                             gCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCtt     >  8:207325/1‑149 (MQ=255)                                                                                                                                                ttGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcg  >  1:152474/1‑149 (MQ=255)                                                                                                                                                  gAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcg  <  5:88914/147‑1 (MQ=255)                                                                                                                                                  gAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcg  <  5:93694/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGC‑GGGCGCGCTC‑GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743220‑5743509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑