Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,750,398	C→T	7.1%	V389M (GTG→ATG)	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,750,398	0	C	T	7.1%	249.0 / 6.5	84	V389M (GTG→ATG)	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (39/39);  new base T (2/4);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

GAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTG  >  NC_002947/5750252‑5750546                                                                                                                                                    |                                                                                                                                                     gAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTCGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATcaca                                                                                                                                                     <  7:253981/148‑1 (MQ=255)   aCGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGa                                                                                                                                                  >  2:114612/1‑149 (MQ=255)       cATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCCCATCGATCATGTGATCGa                                                                                                                                              <  5:312458/149‑1 (MQ=255)        aTGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGAt                                                                                                                                             <  8:244212/149‑1 (MQ=255)         tGGCCTGGAATTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGATg                                                                                                                                            <  3:70780/149‑1 (MQ=255)           gCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATgg                                                                                                                                           <  8:100500/148‑1 (MQ=255)                gACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATg                                                                                                                                      <  3:221309/148‑1 (MQ=255)                gACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATg                                                                                                                                      >  1:111379/1‑148 (MQ=255)                 aCTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGaa                                                                                                                                    >  5:309708/1‑149 (MQ=255)                   ttCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACt                                                                                                                                  >  1:205227/1‑149 (MQ=255)                      gATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTgg                                                                                                                               >  6:72863/1‑149 (MQ=255)                      gATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTg                                                                                                                                <  6:313841/148‑1 (MQ=255)                      gATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTg                                                                                                                                <  5:124048/148‑1 (MQ=255)                      gATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCACGGCCACTTC‑CTCGATTTCGATGACCTTGCCAAGCTCAACGATCACATGATCGATGGAAATGAACtagt                                                                                                                               >  7:291964/1‑146 (MQ=255)                       aTACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgc                                                                                                                              <  3:260703/149‑1 (MQ=255)                           cTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTg                                                                                                                                >  8:251879/1‑143 (MQ=255)                           cTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTg                                                                                                                                <  7:251879/143‑1 (MQ=255)                           cTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgcgcgc                                                                                                                          >  3:79668/1‑149 (MQ=255)                              ccAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTc                                                                                                                        >  6:240352/1‑148 (MQ=255)                                aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTc                                                                                                                        >  2:124606/1‑146 (MQ=255)                                aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTc                                                                                                                        >  3:63816/1‑146 (MQ=255)                                 gCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGAt                                                                                                                                             >  4:25779/1‑124 (MQ=255)                                 gCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGAt                                                                                                                                             <  3:25779/124‑1 (MQ=255)                                 gCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                    <  5:72863/149‑1 (MQ=255)                                     cAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgc                                                                                                                >  3:216059/1‑149 (MQ=255)                                       gagGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCACTCAAGAgcgcgc                                                                                                              >  1:128229/1‑149 (MQ=255)                                          gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTcc                                                                                                           >  7:106478/1‑149 (MQ=255)                                           ttCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTcc                                                                                                           <  2:111379/148‑1 (MQ=255)                                              gtattCCTTGGCTTCGGGGTGTCGAATGTCTATGACGATCTGGCCTGGGAGCGCCTCTGCCAATTC‑CTCTATTTCGATGTCCTTGCCCAGCTCATCTATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGc                                                                                                        <  6:42583/145‑1 (MQ=255)                                                   ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                   >  1:90543/1‑148 (MQ=255)                                                      gggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATg                                                                                               >  7:181219/1‑149 (MQ=255)                                                       ggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATg                                                                                               <  1:124606/148‑1 (MQ=255)                                                          aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGaa                                                                                                                                    >  6:273978/1‑108 (MQ=255)                                                          aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGaa                                                                                                                                    <  5:273978/108‑1 (MQ=255)                                                              gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTg                                                                                       >  2:154656/1‑149 (MQ=255)                                                                gTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCt                                                                                     <  2:205227/149‑1 (MQ=255)                                                                      aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAGGTCGAACTGCTTttctt                                                                               >  8:311352/1‑149 (MQ=255)                                                                       aTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                              <  8:106478/149‑1 (MQ=255)                                                                       aTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                              <  5:189907/149‑1 (MQ=255)                                                                        tGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGTTCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCg                                                                             >  7:195272/1‑149 (MQ=255)                                                                         gTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCTCCCCATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                              <  1:114612/148‑1 (MQ=255)                                                                           cGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACg                                                                                                     <  3:133461/122‑1 (MQ=255)                                                                            gATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCt                                                                         <  8:181219/149‑1 (MQ=255)                                                                               gACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCa                                                                      >  3:20666/1‑149 (MQ=255)                                                                                        gcctggcAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                  <  3:34070/144‑1 (MQ=255)                                                                                        gcctggcAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGt                                                                <  4:63816/146‑1 (MQ=255)                                                                                        gcctggcAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGt                                                                <  6:19748/146‑1 (MQ=255)                                                                                          ctggcAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCACTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGc                                                           <  2:128229/149‑1 (MQ=255)                                                                                             gcAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTg                                                        <  5:240352/149‑1 (MQ=255)                                                                                                gcgcCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCa                                                     <  6:207546/149‑1 (MQ=255)                                                                                                   ccTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                  <  3:75625/149‑1 (MQ=255)                                                                                                   ccTCGGCCACTTC‑CTAGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                  <  7:267921/149‑1 (MQ=255)                                                                                                    cTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCgg                                                                 >  4:294923/1‑133 (MQ=255)                                                                                                    cTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCgg                                                                 <  3:294923/133‑1 (MQ=255)                                                                                                       ggCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtgg                                              >  2:139302/1‑149 (MQ=255)                                                                                                           aCTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                          <  4:20666/149‑1 (MQ=255)                                                                                                           aCTTA‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATAGATGGAAATGAACTTGGCGGGCTGAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                          <  8:291964/149‑1 (MQ=255)                                                                                                              tC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGtt                                       >  3:322672/1‑149 (MQ=255)                                                                                                                 cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                  <  6:88530/136‑1 (MQ=255)                                                                                                                 cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGAGATCggaag                                       >  5:88530/1‑144 (MQ=255)                                                                                                                  tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAc                                    >  3:137420/1‑149 (MQ=255)                                                                                                                    gATTTCGATGTCCTTGCCCAGCTCATCGTTCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                   <  8:195272/148‑1 (MQ=255)                                                                                                                    gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                   >  1:238430/1‑148 (MQ=255)                                                                                                                     aTTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtgg                                              <  4:159927/136‑1 (MQ=255)                                                                                                                     aTTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtgg                                              >  3:159927/1‑136 (MQ=255)                                                                                                                     aTTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCga                                 <  1:98509/149‑1 (MQ=255)                                                                                                                          gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTc                                                                        <  2:184983/105‑1 (MQ=255)                                                                                                                          gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTc                                                                        >  1:184983/1‑105 (MQ=255)                                                                                                                          gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCa                            >  4:282835/1‑149 (MQ=255)                                                                                                                          gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCa                            >  6:76131/1‑149 (MQ=255)                                                                                                                           aTGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                          <  8:57382/134‑1 (MQ=255)                                                                                                                           aTGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                          >  7:57382/1‑134 (MQ=255)                                                                                                                           aTGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATCGGTGGTCGGGTTTACCGAGATCAc                           >  6:240870/1‑149 (MQ=255)                                                                                                                                cTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccg                       >  7:10339/1‑148 (MQ=255)                                                                                                                                    cccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTa                  <  8:10339/149‑1 (MQ=255)                                                                                                                                        gCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTcc              >  3:310831/1‑149 (MQ=255)                                                                                                                                          tCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCGTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCgg            >  4:20525/1‑149 (MQ=255)                                                                                                                                           cATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCgg            >  4:255682/1‑148 (MQ=255)                                                                                                                                            aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa          >  7:68113/1‑149 (MQ=255)                                                                                                                                            aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa          >  3:324935/1‑149 (MQ=255)                                                                                                                                             tCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt         <  3:255682/149‑1 (MQ=255)                                                                                                                                             tCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt         <  7:96636/149‑1 (MQ=255)                                                                                                                                             tCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt         <  6:309708/149‑1 (MQ=255)                                                                                                                                             tCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt         <  1:119519/149‑1 (MQ=255)                                                                                                                                             tCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt         >  7:100774/1‑149 (MQ=255)                                                                                                                                                   acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCtt   <  2:90543/149‑1 (MQ=255)                                                                                                                                                    catcatCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg  <  4:216059/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTC‑CTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTG  >  NC_002947/5750252‑5750546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑