Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,761,211	T→C	6.2%	I88T (ATC→ACC)	gpmI →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,761,211	0	T	C	6.2%	187.8 / 2.6	64	I88T (ATC→ACC)	gpmI	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (31/29);  new base C (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

ACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATGGC  >  NC_002947/5761069‑5761352                                                                                                                                                |                                                                                                                                              aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGa                                                                                                                                         <  2:265614/149‑1 (MQ=255) aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGa                                                                                                                                         >  7:23020/1‑149 (MQ=255) aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGATGGCCTTGGTCACCcgcgt                                                                                                                                         <  8:81127/149‑2 (MQ=255) aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGATGGCCTTGGTCACCcgcgt                                                                                                                                         <  7:130614/149‑2 (MQ=255)   ccAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACg                                                                                                                                       >  1:338948/1‑149 (MQ=255)   ccAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACg                                                                                                                                       >  8:154178/1‑149 (MQ=255)    cAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACgg                                                                                                                                      >  1:31126/1‑149 (MQ=255)       ccGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCTACGGCGa                                                                                                                                   <  7:154178/149‑1 (MQ=255)              gCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            <  4:153795/149‑1 (MQ=255)              gCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            <  6:194379/149‑1 (MQ=255)              gCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            <  5:333513/149‑1 (MQ=255)               ccTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            >  4:147296/1‑148 (MQ=255)               ccTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            <  3:147296/148‑1 (MQ=255)                   aTTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGaaa                                                                                                                        <  1:296389/148‑1 (MQ=255)                    tttCCGGCTCGGGCATGGATGTCGGCCTGACGGAAGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAAc                                                                                                                      >  6:332084/1‑149 (MQ=255)                          gCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTa                                                                                                                >  3:169490/1‑149 (MQ=255)                              gggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGa                                                                                                            <  8:23020/149‑1 (MQ=255)                                gCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAcc                                                                                                          >  7:246526/1‑149 (MQ=255)                                gCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAcc                                                                                                          >  2:77570/1‑149 (MQ=255)                                gCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAcc                                                                                                          >  6:125385/1‑149 (MQ=255)                                    ggATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                           >  8:215085/1‑144 (MQ=255)                                    ggATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                           <  7:215085/144‑1 (MQ=255)                                    ggATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCgg                                                                                                        <  3:203877/147‑1 (MQ=255)                                    ggATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCgg                                                                                                        >  4:203877/1‑147 (MQ=255)                                         tCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgt                                                                                                  <  5:166059/148‑1 (MQ=255)                                           ggCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACAGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGa                                                                                               >  3:238509/1‑149 (MQ=255)                                              cTGGCGGACGGGCAGATGGGCAACTCCGTAGTCGTTCATATGATAGTGGGCGCAGGTCGCGTTGTGTATGAGGACTT‑CACTCTGGTGTCCATGGCCATGCGTGACTGCGTGTTCTTGTATAAACCTGTAGTGTCCGGTGCTGTTGatta                                                                                            <  4:238509/149‑4 (MQ=255)                                                 ccGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAgg                                                                                          <  2:338948/148‑1 (MQ=255)                                                 ccGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAg                                                                                           <  2:131184/147‑1 (MQ=255)                                                 ccGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAg                                                                                           >  1:131184/1‑147 (MQ=255)                                                  cGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCa                                                                                        <  3:120010/149‑1 (MQ=255)                                                      cGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCg                                                                                    <  4:169490/149‑1 (MQ=255)                                                         gCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAcc                                                                                                          >  6:322479/1‑124 (MQ=255)                                                         gCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAcc                                                                                                          <  5:322479/124‑1 (MQ=255)                                                         gCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgt                                                                                                  >  5:117637/1‑132 (MQ=255)                                                         gCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgt                                                                                                  <  6:117637/132‑1 (MQ=255)                                                          cAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGc                                                                                <  2:31126/149‑1 (MQ=255)                                                           aGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCt                                                                               >  2:188969/1‑149 (MQ=255)                                                           aGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGAGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCt                                                                               >  8:210021/1‑149 (MQ=255)                                                              tGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACaa                                                                                            <  3:198910/133‑1 (MQ=255)                                                              tGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACaa                                                                                            >  4:198910/1‑133 (MQ=255)                                                               gggCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            <  6:14475/100‑1 (MQ=255)                                                               gggCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttcttc                                                                                                                            >  5:14475/1‑100 (MQ=255)                                                                ggCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCaa                                                                          <  5:125385/149‑1 (MQ=255)                                                                ggCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCaa                                                                          <  5:332084/149‑1 (MQ=255)                                                                 gCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAg                                                                         >  5:282555/1‑149 (MQ=255)                                                                                       gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTACACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGActg                                                    >  1:56489/1‑149 (MQ=255)                                                                                       gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAgg                                                                                          >  6:265739/1‑110 (MQ=255)                                                                                       gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAgg                                                                                          <  5:265739/110‑1 (MQ=255)                                                                                                cgcAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtg                                          >  8:164326/1‑149 (MQ=255)                                                                                                   aGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                       <  1:77570/149‑1 (MQ=255)                                                                                                              tgtATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGc                            >  4:229458/1‑149 (MQ=255)                                                                                                                tATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                       <  8:286290/136‑1 (MQ=255)                                                                                                                tATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                       >  7:286290/1‑136 (MQ=255)                                                                                                                 aTCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGccac                         >  5:48969/1‑149 (MQ=255)                                                                                                                  tCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCCGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGccac                         <  8:246526/148‑1 (MQ=255)                                                                                                                         tt‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCa                 >  3:189472/1‑149 (MQ=255)                                                                                                                           cgtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCAc                >  7:19803/4‑149 (MQ=255)                                                                                                                            gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACt               >  1:292940/3‑149 (MQ=255)                                                                                                                                  ggTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg         <  7:164326/149‑1 (MQ=255)                                                                                                                                       ccAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATg    >  8:268669/1‑149 (MQ=255)                                                                                                                                        cAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg         <  2:131244/143‑1 (MQ=255)                                                                                                                                        cAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg         >  1:131244/1‑143 (MQ=255)                                                                                                                                         aaGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATGGc  >  7:8303/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                              ACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTT‑CACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATGGC  >  NC_002947/5761069‑5761352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑