Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,768,424 C→T 6.9% M816I (ATG→ATA)  PP_5060 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,768,4240CT 6.9% 173.6 / 2.4 58M816I (ATG→ATA) PP_5060hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base T (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CCAGCCCCGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCGGCGTC  >  NC_002947/5768276‑5768571
                                                                                                                                                    |                                                                                                                                                   
ccAGCCCCGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGcc                                                                                                                                                     <  2:250699/149‑1 (MQ=255)
 cAGCCCCGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCa                                                                                                                                                    <  3:151408/149‑1 (MQ=255)
  aGCCCCGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCAt                                                                                                                                                   >  4:198534/1‑149 (MQ=255)
    ccccGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCg                                                                                                                                                 <  6:311490/149‑1 (MQ=255)
             cacaCCCTGCGTCTGCGGGGCGCGCCCTTCCAACGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGc                                                                                                                                        >  2:60854/1‑149 (MQ=255)
                 cccTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTc                                                                                                                                    <  4:247740/149‑1 (MQ=255)
                  ccTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTc                                                                                                                                    >  3:182844/1‑148 (MQ=255)
                        ccggccgggCGCGCCCATCCAGCGACACACAGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCgag                                                                                                                             <  4:213618/143‑1 (MQ=255)
                         cTGCGGGGCGCGCCCTTCCAGCGCAACACTGCCCCCCAACGCCCGCAGCTGGCCTCGCTGGGCACTTGACAGGCCATGGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGCGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCgaga                                                                                                                            <  6:224056/149‑1 (MQ=255)
                                gcgcgcCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCCGCCCCACGCCCGGAAAAGTCGAGGTGGGTGTTGGCTATCGCCTGCAGGCTGTCGACCGAGCCGCTggg                                                                                                                     >  1:72545/1‑149 (MQ=255)
                                gcgcgcCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCTATCGCCTGCAGGCTGTCGACCGAGACGCTggg                                                                                                                     >  1:77313/1‑149 (MQ=255)
                                 cgcgcCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGc                                                                                                                    >  7:235419/1‑149 (MQ=255)
                                   cgcCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTc                                                                                                                  >  5:253768/1‑149 (MQ=255)
                                     cccTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTccc                                                                                                                <  3:198534/149‑1 (MQ=255)
                                      ccTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCcg                                                                                                               >  1:198379/1‑149 (MQ=255)
                                      ccTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCcg                                                                                                               <  8:90878/149‑1 (MQ=255)
                                       cTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCcgc                                                                                                              <  5:74474/149‑1 (MQ=255)
                                        ttCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCGCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGAcc                                                                                                                                <  1:33614/130‑1 (MQ=255)
                                        ttCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCGCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGAcc                                                                                                                                >  2:33614/1‑130 (MQ=255)
                                               gAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGt                                                                                                      <  4:92940/149‑1 (MQ=255)
                                                aaaCACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTg                                                                                                     <  7:226074/149‑1 (MQ=255)
                                                   cacTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGaa                                                                                                  <  2:107975/149‑1 (MQ=255)
                                                               aCGCCCGCATCCGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGc                                                                                      >  7:263498/1‑149 (MQ=255)
                                                                 gCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTc                                                                                    >  5:31966/1‑149 (MQ=255)
                                                                 gCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGCCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTc                                                                                    >  7:294759/1‑149 (MQ=255)
                                                                    cGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCg                                                                                           <  1:87568/139‑1 (MQ=255)
                                                                    cGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCg                                                                                           >  2:87568/1‑139 (MQ=255)
                                                                    cGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCg                                                                                           >  4:234758/1‑139 (MQ=255)
                                                                    cGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCg                                                                                           <  3:234758/139‑1 (MQ=255)
                                                                     gCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATc                                                                                >  6:131858/1‑149 (MQ=255)
                                                                       aTCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATccc                                                                              <  2:198379/149‑1 (MQ=255)
                                                                             ccTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGcc                                                                        <  4:182844/149‑1 (MQ=255)
                                                                              cTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATAGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCt                                                                       >  7:257237/1‑149 (MQ=255)
                                                                                 gCTGGGCACTTGACAGGCCATTGCGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTg                                                                      <  8:235419/147‑1 (MQ=255)
                                                                                     ggCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTg                                                                >  1:136084/1‑149 (MQ=255)
                                                                                               aGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGag                                                      >  7:64486/1‑149 (MQ=255)
                                                                                                 gCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGagca                                                    <  6:31966/149‑1 (MQ=255)
                                                                                                       gAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGAt                                               <  8:107645/148‑1 (MQ=255)
                                                                                                            ccccAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTAGATCCCATCGCCTGCAGCTGGCCCCTGGAGCACCGATggagg                                          >  4:195300/1‑148 (MQ=255)
                                                                                                            ccccAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGGGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATggcgg                                          >  5:27033/1‑148 (MQ=255)
                                                                                                             cccAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATggcgg                                          >  1:261950/1‑147 (MQ=255)
                                                                                                             cccAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCa                                        <  1:60854/149‑1 (MQ=255)
                                                                                                              ccAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAg                                       <  5:131858/149‑1 (MQ=255)
                                                                                                               cAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAgg                                      <  6:253768/149‑1 (MQ=255)
                                                                                                                    ccACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATggcg                                           >  5:193439/1‑139 (MQ=255)
                                                                                                                    ccACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATggcg                                           <  6:193439/139‑1 (MQ=255)
                                                                                                                      aCGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGc                                  >  7:258535/1‑146 (MQ=255)
                                                                                                                          cAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGAc                           >  6:32549/1‑149 (MQ=255)
                                                                                                                             gAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGAcggg                        >  3:178665/1‑149 (MQ=255)
                                                                                                                                  gTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGAcgggcggga                   >  4:182870/1‑148 (MQ=255)
                                                                                                                                     gAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTg                >  5:88628/1‑149 (MQ=255)
                                                                                                                                       ggtgggtgTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGc               >  4:156497/1‑148 (MQ=255)
                                                                                                                                           ggtgTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGtt          >  2:290055/1‑149 (MQ=255)
                                                                                                                                             tgtTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTc        <  8:263498/149‑1 (MQ=255)
                                                                                                                                              gtTGGCTATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCg       <  2:72545/149‑1 (MQ=255)
                                                                                                                                               ttGGCTATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTTCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCg       <  2:77313/148‑1 (MQ=255)
                                                                                                                                                 ggCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCGGc     <  8:64486/148‑1 (MQ=255)
                                                                                                                                                  gCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCGGCGt   >  5:304178/1‑149 (MQ=255)
                                                                                                                                                  gCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCGGCGt   <  8:258535/149‑1 (MQ=255)
                                                                                                                                                   ccAACGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCATCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGCCGGGCGGGGCTGCCAGTTTCGGCGTc  >  8:137886/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCCCCGCGGCCACACCCTGCGTCTGCGGGGCGCGCCCTTCCAGCGAAACACTGGCCGCCAACGCCCGCATCTGGCCTCGCTGGGCACTTGACAGGCCATTGAGCCCCCCAGCCCCACGCCAGGAAAAGTCGAGGTGGGTGTTGGCCATCGCCTGCAGGCTGTCGACCGAGACGCTGGGCTCCCGCGTCGCGGTGGAAAGGTTCGCCAGCTCGATCCCATCGCCTGCAGCTGGCCCCTGGAGCAGCGATGGCGGCAGGTCGCCAGAGACGGGCGGGGCTGCCAGTTTCGGCGTC  >  NC_002947/5768276‑5768571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑