Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,788,011 C→T 8.3% R253H (CGC→CAC)  ynfL ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,788,0110CT 8.3% 131.8 / 2.3 48R253H (CGC→CAC) ynfLDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01

CCGGCATCACGCCTGACCGACCACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCAATTGTG  >  NC_002947/5787863‑5788149
                                                                                                                                                    |                                                                                                                                          
ccGGCATCACGCCTGACCGACCACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGatgc                                                                                                                                            <  4:257583/149‑1 (MQ=255)
       cACGCCTGACCGACCAACTGCCCTCACAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCAACACCGCCGTCATCGCCTCTTCATCGAGCAAGCTGCGCTACACCACCACTTCGatgcgcaagcg                                                                                                                                     >  8:26900/1‑149 (MQ=255)
           ccTGACCGACCACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTgg                                                                                                                                 <  7:113628/149‑1 (MQ=255)
               accgaccACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAac                                                                                                                              >  3:322492/1‑148 (MQ=255)
               accgaccACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGCACACCACCCCTTCGATGCGCATGCGCTGGAac                                                                                                                              <  6:96579/148‑1 (MQ=255)
                ccgaccACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGCGCAAGGTGCGGTACACCACCCCTACGATCCGCATGCTCTGGAacga                                                                                                                            >  4:257357/1‑149 (MQ=255)
                    ccaccTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGatgcgcatgcg                                                                                                                                     >  1:207851/1‑136 (MQ=255)
                    ccaccTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGatgcgcatgcg                                                                                                                                     <  2:207851/136‑1 (MQ=255)
                      accTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCgg                                                                                                                      >  8:6365/1‑149 (MQ=255)
                       ccTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCgg                                                                                                                      >  6:338285/1‑148 (MQ=255)
                             cTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACAcc                                                                                                               >  3:280504/1‑149 (MQ=255)
                              agTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATCACCACGCTCGCGCTGCACCAGCCACACCGCAGTCATCGCCTCTTCATAGAGCAACGAGCGCTACACCCCCCCTTCGAAGCGCATGCCCTGGAACCACGCCGCCAACAcc                                                                                                               >  8:246782/2‑148 (MQ=255)
                                  aacaacTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGacac                                                                                                          >  1:38362/1‑149 (MQ=255)
                                   acaacTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGTGCATGCGCTGGAACGACGCCGGCAACACCGACAcc                                                                                                         >  3:108440/1‑149 (MQ=255)
                                     aacTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGTGCATGCGCTGGAACGACGCCGGCAACACCGACAcccc                                                                                                       >  5:276787/1‑149 (MQ=255)
                                       cTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCTAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCa                                                                                                     >  5:242448/1‑149 (MQ=255)
                                        tCGGCAAATGCCTTGGCCATGACCGAATGCCCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAg                                                                                                    >  5:71572/1‑149 (MQ=255)
                                        tCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAg                                                                                                    >  7:41591/1‑149 (MQ=255)
                                                   cTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCgaga                                                                                         <  7:211435/149‑1 (MQ=255)
                                                    ttGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCCACACCGACACCCCCAGCCCCGCCGAGAc                                                                                        >  7:96398/1‑149 (MQ=255)
                                                      ggCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGAcc                                                                                       <  2:38362/148‑1 (MQ=255)
                                                       gCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCa                                                                                      <  4:280504/148‑1 (MQ=255)
                                                         cATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGTGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGc                                                                                   <  6:276787/149‑1 (MQ=255)
                                                           tGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCTCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCg                                                                                 <  5:254423/149‑1 (MQ=255)
                                                              ccGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCgat                                                                               <  4:322492/148‑1 (MQ=255)
                                                                     aCCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGCGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACGCCCAGCCCCGCCGAGACCAGGCCGATGATGGTc                                                                        <  3:257357/148‑1 (MQ=255)
                                                                      ccACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCAt                                                                      <  7:26900/149‑1 (MQ=255)
                                                                       cACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCGAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATc                                                                     <  4:89816/149‑1 (MQ=255)
                                                                       cACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATgg                                                                          >  2:152339/1‑144 (MQ=255)
                                                                       cACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATgg                                                                          <  1:152339/144‑1 (MQ=255)
                                                                       cACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATc                                                                     >  1:183024/1‑149 (MQ=255)
                                                                       cACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCGCTTCATCGAGCAAGGTGCGGTACGCCACCCCTTCGATGCGCATGCGCTGGAACGACGCGGGCAACGCGGGGGCCGCCAGCCCCGCCGGGACGAGGCCGATGATggggttg                                                                     >  4:37283/1‑144 (MQ=255)
                                                                                gcTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCACGCCGATGATGGTCATCGCTTCCCCt                                                            >  8:300401/1‑149 (MQ=255)
                                                                                  tGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGatgc                                                                                                                                            >  4:192404/1‑67 (MQ=255)
                                                                                  tGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGatgc                                                                                                                                            <  3:192404/67‑1 (MQ=255)
                                                                                   gCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCt                                                            >  8:76940/1‑146 (MQ=255)
                                                                                   gCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCt                                                            <  7:76940/146‑1 (MQ=255)
                                                                                      ccAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCctct                                                      >  4:228088/1‑149 (MQ=255)
                                                                                       cAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCtt                                                     <  8:41329/149‑1 (MQ=255)
                                                                                       cAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCtt                                                     <  3:37283/149‑1 (MQ=255)
                                                                                                        tCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCt                                     >  3:113515/1‑148 (MQ=255)
                                                                                                           ccTCTTCATCGAGCAACGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGaaa                                 <  7:246782/149‑1 (MQ=255)
                                                                                                            ctctTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAAc                                <  7:6365/149‑1 (MQ=255)
                                                                                                                  aTCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCa                           <  6:242448/148‑1 (MQ=255)
                                                                                                                      aGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCg                      >  7:6409/1‑149 (MQ=255)
                                                                                                                         aaGGTGCGGTACACCACCCCTTCGATGTGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGc                   <  4:108440/149‑1 (MQ=255)
                                                                                                                              gCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGc              >  8:260538/1‑149 (MQ=255)
                                                                                                                                ggTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTc            <  2:183024/149‑1 (MQ=255)
                                                                                                                                 gTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCa           <  8:41591/149‑1 (MQ=255)
                                                                                                                                     acccccCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCGTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCaa       >  1:50129/1‑149 (MQ=255)
                                                                                                                                     accaccCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCaa       >  8:192021/1‑149 (MQ=255)
                                                                                                                                        accCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCTAAATGGGGGCTGAACCCCGCATGCCGTGCCCGGCTCCGCAATtg    >  4:48483/1‑149 (MQ=255)
                                                                                                                                         ccccTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGATACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCAATtgt   <  5:338285/149‑1 (MQ=255)
                                                                                                                                          cagttCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCAATtgtg  <  4:329252/146‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
CCGGCATCACGCCTGACCGACCACCTGCCCTGTCAACAACTCGGCAAATGCCTTGGCCATGACCGAATGACCACGCTCGCGCTGCACCAGCCACACCGCCGTCATCGCCTCTTCATCGAGCAAGGTGCGGTACACCACCCCTTCGATGCGCATGCGCTGGAACGACGCCGGCAACACCGACACCCCCAGCCCCGCCGAGACCAGGCCGATGATGGTCATCGCTTCCCCTGCCTCTTGCGCAAAATGGGGGCTGAAACCCGCATGCCGTGCCAGGCTCAGCAATTGTG  >  NC_002947/5787863‑5788149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑