Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,964 G→T 7.3% P300Q (CCA→CAA)  PP_5077 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,9640GT 7.3% 215.2 / 6.2 68P300Q (CCA→CAA) PP_5077hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base T (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

CGGGCCTCCCTCTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCATGGTTGGTCGCTT  >  NC_002947/5798816‑5799112
                                                                                                                                                     |                                                                                                                                                    
cGGGCCTCCCTCTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTg                                                                                                                                                      <  5:181301/149‑1 (MQ=255)
cGGGCCTCCCTCTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTg                                                                                                                                                      <  4:20685/149‑1 (MQ=255)
   gCCTCCCTCTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTTGcc                                                                                                                                                   >  5:279454/1‑148 (MQ=255)
    cctccctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCaa                                                                                                                                                  <  7:188447/149‑1 (MQ=255)
     ctccctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAAc                                                                                                                                                 >  1:188452/1‑149 (MQ=255)
     ctccctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAAc                                                                                                                                                 >  4:160277/1‑149 (MQ=255)
      tccctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑CGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAAct                                                                                                                                                >  5:216621/1‑149 (MQ=255)
       ccctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActg                                                                                                                                               >  4:75242/1‑149 (MQ=255)
        cctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActgg                                                                                                                                              >  8:53024/1‑149 (MQ=255)
        cctcTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActg                                                                                                                                               <  5:39718/148‑1 (MQ=255)
            ttCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTTGCCActgggc                                                                                                                                            <  5:138278/147‑1 (MQ=255)
              cACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActgggcct                                                                                                                                          <  8:98701/147‑1 (MQ=255)
               aCCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCTTTGGCTCCAGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTTGCCActgggcgacg                                                                                                                                        <  1:102056/149‑5 (MQ=255)
                cccTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTTGCCActgggcgacggc                                                                                                                                      <  6:279454/149‑7 (MQ=255)
                 ccTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActgggcctggg                                                                                                                                       >  2:238520/1‑147 (MQ=255)
                 ccTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAActgggcctggg                                                                                                                                       >  3:163762/1‑147 (MQ=255)
                  cTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAg                                                                                                                                    >  1:327999/1‑149 (MQ=255)
                   tCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGc                                                                                                                                   >  5:249349/1‑149 (MQ=255)
                     gCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCg                                                                                                                                 <  8:258612/149‑1 (MQ=255)
                        cATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGgcg                                                                                                                              >  3:6331/1‑149 (MQ=255)
                            ccTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTc                                                                                                                          >  3:277031/1‑149 (MQ=255)
                             cTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCg                                                                                                                         <  3:172170/149‑1 (MQ=255)
                                 ggCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGTAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAg                                                                                                                     <  4:123422/149‑1 (MQ=255)
                                 ggCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAg                                                                                                                     <  6:266933/149‑1 (MQ=255)
                                   cTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAggg                                                                                                                   >  1:214235/1‑149 (MQ=255)
                                         ccAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAggg                                                                                                                   <  5:92559/143‑1 (MQ=255)
                                         ccAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAggg                                                                                                                   >  6:92559/1‑143 (MQ=255)
                                          cAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGt                                                                                                            >  7:277602/1‑149 (MQ=255)
                                           aGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATCGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGTGCCTTGTc                                                                                                           <  3:75242/149‑1 (MQ=255)
                                             cGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGc                                                                                                         <  8:277602/149‑1 (MQ=255)
                                                cTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGTCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCtt                                                                                                      <  6:216621/149‑1 (MQ=255)
                                                   gcgcATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTc                                                                                                    <  3:107169/148‑1 (MQ=255)
                                                       aTCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTggg                                                                                               >  1:300644/1‑149 (MQ=255)
                                                       aTCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTggg                                                                                               >  8:84247/1‑149 (MQ=255)
                                                             ggCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAggt                                                                                                                   <  8:132682/123‑2 (MQ=255)
                                                             ggCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAggg                                                                                                                   >  7:132682/1‑123 (MQ=255)
                                                                   gACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTgg                                                                                                <  4:190201/136‑1 (MQ=255)
                                                                   gACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTgg                                                                                                >  3:190201/1‑136 (MQ=255)
                                                                    aCTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCTGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACcgcg                                                                                  >  1:109323/1‑149 (MQ=255)
                                                                     cTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCgg                                                                                 >  1:156028/1‑149 (MQ=255)
                                                                          cAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGc                                                                             >  3:19940/1‑148 (MQ=255)
                                                                           aaCCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCaa                                                                           <  4:238814/149‑1 (MQ=255)
                                                                                   gCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAAc                                                                    >  8:287256/1‑148 (MQ=255)
                                                                                   gCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAAc                                                                    >  3:116912/1‑148 (MQ=255)
                                                                                     aGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTg                                                                                                 <  5:77933/117‑1 (MQ=255)
                                                                                     aGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTg                                                                                                 >  6:77933/1‑117 (MQ=255)
                                                                                               gCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCg                                                       <  4:163762/149‑1 (MQ=255)
                                                                                                      aCGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAgc                                                 >  1:118979/1‑149 (MQ=255)
                                                                                                          gCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCt                                                                                                   >  1:178500/1‑95 (MQ=255)
                                                                                                          gCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCt                                                                                                   <  2:178500/95‑1 (MQ=255)
                                                                                                          gCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGa                                             <  7:53024/149‑1 (MQ=255)
                                                                                                          gCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGa                                             <  3:160277/149‑1 (MQ=255)
                                                                                                             agagAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGca                                          >  1:100823/1‑149 (MQ=255)
                                                                                                                 aaCTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATg                                      >  5:48759/1‑149 (MQ=255)
                                                                                                                      gATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGTGAGCACATGttttt                                 >  2:165732/1‑149 (MQ=255)
                                                                                                                      gATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGttttt                                 <  7:287256/149‑1 (MQ=255)
                                                                                                                        tGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTa                               <  2:327999/149‑1 (MQ=255)
                                                                                                                        tGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTa                               <  2:214235/149‑1 (MQ=255)
                                                                                                                         ggCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCGACGGCAACCACGACCACAGAGGACAGCGCGAGCACATGTTTTTTAg                              >  2:71532/1‑149 (MQ=255)
                                                                                                                          gcagcaCCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAgg                             <  4:6331/149‑1 (MQ=255)
                                                                                                                          gcagcaCCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAgg                             >  7:36753/1‑149 (MQ=255)
                                                                                                                            agcaCCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCa                           >  6:119307/1‑149 (MQ=255)
                                                                                                                              caCCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATc                         <  4:277031/149‑1 (MQ=255)
                                                                                                                                 ccTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCtt                       >  4:36914/1‑148 (MQ=255)
                                                                                                                                     cTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAAc                   <  2:118979/148‑1 (MQ=255)
                                                                                                                                            aggCCCAGTTGCCACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCAtggt           >  6:99293/3‑149 (MQ=255)
                                                                                                                                              gCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCAtggtt          <  2:188452/148‑1 (MQ=255)
                                                                                                                                                ccAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCAtggttggt       >  6:308189/1‑149 (MQ=255)
                                                                                                                                                ccAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCAtggttggt       <  3:78810/149‑1 (MQ=255)
                                                                                                                                                   gTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGGGCGAGCACATGTTTTTTAGGCATCTTGAACCCCATGGTTGGTCGc    <  1:238520/149‑1 (MQ=255)
                                                                                                                                                     ggCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCATGGTTGGTCGCtt  <  2:109323/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGGCCTCCCTCTTCACCCTCGCCCATGCCTGCGGCTTGGGCCAGCGGCTCGCGCATCACCGGCTGCGACTGGCCAACCAGTGGCAGCGGCATTGGCT‑GGGACGAGCCAGAGAACTCGATGGCAGCACCCTGCTTGCCGTCGCCCAGTGGCAACTGGGCCTGGGCAGCCGGCGCTTCGGCAGGGGCCTTGTCGCCTTTCTTGGGCATCAATACCGCGGCCGCAACGGCAACCACGACCACAGCGGACAGCGCGAGCACATGTTTTTTAGGCATCTTGAACCCCATGGTTGGTCGCTT  >  NC_002947/5798816‑5799112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑