Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,866,696 G→T 13.7% intergenic (+19/+38) PP_5140 → / ← thyA MerR family transcriptional regulator/thymidylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,866,6960GT13.7% 174.1 / 17.0 65intergenic (+19/+38)PP_5140/thyAMerR family transcriptional regulator/thymidylate synthase
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base T (6/3);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

GGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTC  >  NC_002947/5866551‑5866841
                                                                                                                                                 |                                                                                                                                                 
ggAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAAc                                                                                                                                                >  1:170638/1‑149 (MQ=255)
   gCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcg                                                                                                                                             >  5:86015/1‑149 (MQ=255)
    cTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgc                                                                                                                                            >  3:99706/1‑149 (MQ=255)
      gcggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCcgcg                                                                                                                                           >  1:2609/1‑148 (MQ=255)
       cggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGaa                                                                                                                                         <  8:53293/149‑1 (MQ=255)
           ggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagctt                                                                                                                                     <  2:2609/149‑4 (MQ=255)
              gcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGt                                                                                                                                    >  3:220252/1‑147 (MQ=255)
                   aaCGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTc                                                                                                                              >  2:9368/1‑148 (MQ=255)
                         cAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAAt                                                                                                                       <  1:308933/149‑1 (MQ=255)
                          aGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATa                                                                                                                      <  7:166260/149‑1 (MQ=255)
                          aGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGAAAAAAGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCAAGGGCATTGAGCCAAAAGCTTCGCGGTTTAACCAGCGAAGAGGTCAGTTCTAACAAt                                                                                                                       >  8:130413/1‑148 (MQ=255)
                           ggggAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACaa                                                                                                                        >  4:43999/1‑146 (MQ=255)
                            gggAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                    <  1:277853/149‑1 (MQ=255)
                               aGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCTAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGAt                                                                                                                 <  3:147326/149‑1 (MQ=255)
                                   aaTGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTa                                                                                                              >  5:322709/1‑148 (MQ=255)
                                             cTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAgaga                                                                                                                  <  7:67295/134‑1 (MQ=255)
                                             cTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAgaga                                                                                                                  >  8:67295/1‑134 (MQ=255)
                                                gcagcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCg                                                                                                >  4:311858/1‑149 (MQ=255)
                                                  agcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgc                                                                                              >  7:45189/1‑149 (MQ=255)
                                                     aaCTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCt                                                                                           <  6:322709/149‑1 (MQ=255)
                                                        tGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGt                                                                                         >  4:209116/1‑148 (MQ=255)
                                                          gagaCAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTAt                                                                                                             >  4:287247/1‑126 (MQ=255)
                                                          gagaCAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTAt                                                                                                             <  3:287247/126‑1 (MQ=255)
                                                          gagaCAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCAt                                                                                      >  3:129982/1‑149 (MQ=255)
                                                          gagaCAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCAt                                                                                      >  5:107872/1‑149 (MQ=255)
                                                          gagaCAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCAt                                                                                      <  4:99706/149‑1 (MQ=255)
                                                              cAAACGGGGCGGTATCGGTACCGGATACCTAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgc                                                                                  <  4:129982/149‑1 (MQ=255)
                                                               aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcg                                                                                 >  4:207226/1‑149 (MQ=255)
                                                               aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcg                                                                                 <  8:45189/149‑1 (MQ=255)
                                                                   ggggCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATg                                                                             <  3:311858/149‑1 (MQ=255)
                                                                      gCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCa                                                                                                           <  3:309571/116‑1 (MQ=255)
                                                                      gCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCa                                                                                                           >  4:309571/1‑116 (MQ=255)
                                                                       cGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGc                                                                         <  3:108809/149‑1 (MQ=255)
                                                                              ggTACCGGATACCGAACATTCCCATGTGCGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAg                                                                   <  5:301622/148‑1 (MQ=255)
                                                                               gTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGATTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAAAAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGcc                                                                 >  2:303780/1‑149 (MQ=255)
                                                                                tACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCt                                                                <  4:220252/149‑1 (MQ=255)
                                                                                  ccGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTc                                                              <  4:75419/149‑1 (MQ=255)
                                                                                   cGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTc                                                              >  4:103479/1‑148 (MQ=255)
                                                                                   cGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTc                                                              >  1:92343/1‑148 (MQ=255)
                                                                                         ccGAACATTCCCATGTGGGGCGGAGTCTCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                        <  3:43999/148‑1 (MQ=255)
                                                                                          cGAACATTCCCATGTGGGGCGGAGTCAAGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATACCCTTccagccag                                                       >  7:57771/1‑148 (MQ=255)
                                                                                           gAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGa                                                      >  3:143316/1‑148 (MQ=255)
                                                                                           gAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGa                                                      >  5:53327/1‑148 (MQ=255)
                                                                                               aTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCa                                                 >  2:92236/1‑149 (MQ=255)
                                                                                                  cccATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTc                                              >  2:216756/1‑149 (MQ=255)
                                                                                                          gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCaa                                       <  2:170638/148‑1 (MQ=255)
                                                                                                           ggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtc                                     <  1:9368/149‑1 (MQ=255)
                                                                                                              ggAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtcttc                                  <  4:143316/149‑1 (MQ=255)
                                                                                                                    aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGc                            >  1:148288/1‑149 (MQ=255)
                                                                                                                    aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGc                            <  3:209116/149‑1 (MQ=255)
                                                                                                                    aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGc                            >  5:263299/1‑149 (MQ=255)
                                                                                                                      gggCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCa                          >  8:286405/1‑149 (MQ=255)
                                                                                                                        gCATTGAGCCAATAGCTTCGCGGTTGAAACCTCGAAGATGTAAGTTCTAACAATAGAGATATATCAGACCGCCATCGTCGCTGTCATCGGCGCATTGTGCTCATAGCATTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACt                        <  7:130413/149‑1 (MQ=255)
                                                                                                                           ttGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCg                     <  1:216756/149‑1 (MQ=255)
                                                                                                                                  aaaaGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATa              >  6:234965/1‑149 (MQ=255)
                                                                                                                                   acctcTTCGCGGGTTCACCCGCGAAGCGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATAc             >  6:139947/5‑149 (MQ=255)
                                                                                                                                     ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACtt           >  5:338000/3‑149 (MQ=255)
                                                                                                                                      tcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTg          >  5:127287/2‑149 (MQ=255)
                                                                                                                                       cTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc         >  3:168845/1‑149 (MQ=255)
                                                                                                                                        ttCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc         >  8:311797/1‑148 (MQ=255)
                                                                                                                                         tCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCa       <  1:186937/149‑1 (MQ=255)
                                                                                                                                          cgcgGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGcc        <  2:92343/147‑1 (MQ=255)
                                                                                                                                           gcgGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGt     <  4:168845/149‑1 (MQ=255)
                                                                                                                                             gggTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGttc   <  1:303780/149‑2 (MQ=255)
                                                                                                                                              ggTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTc  <  6:53327/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTC  >  NC_002947/5866551‑5866841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑