Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,902,946 G→A 8.1% P31S (CCT→TCT)  PP_5175 ← HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,902,9460GA 8.1% 211.6 / 5.5 74P31S (CCT→TCT) PP_5175HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base A (4/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTA  >  NC_002947/5902801‑5903088
                                                                                                                                                 |                                                                                                                                              
tGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAg                                                                                                                                             <  5:37568/149‑1 (MQ=255)
 gATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAgt                                                                                                                                            >  4:303834/1‑149 (MQ=255)
   tCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAgtgt                                                                                                                                          <  5:67963/149‑1 (MQ=255)
      cccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGtctc                                                                                                                                       <  6:87186/149‑1 (MQ=255)
      ccaaCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCACCGAAATCGGTGGGTTGTACCTGCAGCACACCCACCCGAGGCACTGtctc                                                                                                                                       >  3:304456/4‑149 (MQ=255)
       ccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                      <  3:303834/149‑1 (MQ=255)
       ccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                      >  7:119485/1‑149 (MQ=255)
              gACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggtg                                                                                                                                  >  4:200990/1‑144 (MQ=255)
              gACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggtg                                                                                                                                  <  3:200990/146‑3 (MQ=255)
               aCGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAATTCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACctc                                                                                                                              <  1:258183/149‑1 (MQ=255)
                 gCTACTCGAAATGTTCTTGCCGCCGACGCTGAATGACAAATCGGTCTGCACGCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGGGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACctct                                                                                                                             <  7:154814/148‑1 (MQ=255)
                   tACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTcc                                                                                                                          >  7:91567/1‑149 (MQ=255)
                    aCGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCt                                                                                                                         >  4:124074/1‑149 (MQ=255)
                    aCGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCt                                                                                                                         >  6:212195/1‑149 (MQ=255)
                      gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                       <  4:320158/149‑1 (MQ=255)
                      gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                   >  2:37187/1‑136 (MQ=255)
                      gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                   <  1:37187/137‑2 (MQ=255)
                       cgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGc                                                                                                                      <  5:212195/149‑1 (MQ=255)
                       cgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGc                                                                                                                      <  6:1031/149‑1 (MQ=255)
                         aaaTGATCTTGCCGCCGACGCGGTATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCgggg                                                                                                                                   <  2:77557/134‑1 (MQ=255)
                         aaaTGATCTTGCCGCCGACGCGGTATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCgggg                                                                                                                                   >  1:77557/1‑134 (MQ=255)
                          aaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                    >  5:307444/1‑148 (MQ=255)
                               tCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCg                                                                                                              >  7:191579/1‑149 (MQ=255)
                                  tGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGt                                                                                                            >  2:217296/1‑148 (MQ=255)
                                   gccgccGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTg                                                                                                           <  1:294167/148‑1 (MQ=255)
                                    ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGa                                                                                                          <  6:129641/148‑1 (MQ=255)
                                    ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                         <  2:96811/149‑1 (MQ=255)
                                    ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                         <  2:3131/149‑1 (MQ=255)
                                            gcgGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTac                                                                                                  >  6:59904/1‑148 (MQ=255)
                                              ggAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                >  8:37349/1‑148 (MQ=255)
                                              ggAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                <  7:37349/148‑1 (MQ=255)
                                               gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaaca                                                                                              <  5:40364/149‑1 (MQ=255)
                                                 aTGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGc                                                                                            <  2:17200/149‑1 (MQ=255)
                                                 aTGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGc                                                                                            <  8:91567/149‑1 (MQ=255)
                                                     cAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCa                                                                                        <  8:191579/149‑1 (MQ=255)
                                                      aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACCCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAg                                                                                       >  7:239555/1‑149 (MQ=255)
                                                      aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTAc                                                                                                                   >  5:260539/1‑121 (MQ=255)
                                                      aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTAc                                                                                                                   <  6:260539/121‑1 (MQ=255)
                                                       aaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGACAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                      <  7:131651/149‑1 (MQ=255)
                                                         tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                         <  1:132348/128‑1 (MQ=255)
                                                         tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                         >  2:132348/1‑128 (MQ=255)
                                                                 cACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAc                                                                              <  6:307444/147‑1 (MQ=255)
                                                                    ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGAACTCTTCCTTGCTACAGCCGCTGAGCATGTACAACATCCCCAGGCAGACAACCGACa                                                                         >  7:292332/1‑149 (MQ=255)
                                                                     cgcgCCTGCACGTGGCCTGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCAGACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAg                                                                        <  4:304456/149‑1 (MQ=255)
                                                                        gcCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGa                                                                                  >  2:211054/1‑136 (MQ=255)
                                                                        gcCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGa                                                                                  <  1:211054/136‑1 (MQ=255)
                                                                              aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                               >  4:106940/1‑149 (MQ=255)
                                                                                gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATc                                                              >  7:215699/1‑148 (MQ=255)
                                                                                        tCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATg                                                     >  6:33245/1‑149 (MQ=255)
                                                                                            aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                 <  3:124074/149‑1 (MQ=255)
                                                                                             gTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                <  5:59904/149‑1 (MQ=255)
                                                                                             gTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                <  8:239555/149‑1 (MQ=255)
                                                                                                aCCCTGGCTGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                      <  7:114782/108‑1 (MQ=255)
                                                                                                aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                      >  8:114782/1‑108 (MQ=255)
                                                                                                aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTc                                              <  6:17350/148‑1 (MQ=255)
                                                                                                  ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                           <  5:33245/149‑1 (MQ=255)
                                                                                                  ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                           >  5:142332/1‑149 (MQ=255)
                                                                                                        aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                     >  5:4735/1‑149 (MQ=255)
                                                                                                          cGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgtg                                    >  6:222507/1‑148 (MQ=255)
                                                                                                                     ggTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGAttt                        <  8:119485/149‑1 (MQ=255)
                                                                                                                     ggTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGATACAGCCGGTGAGCATGTACAAAAGACCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGATGGCTCCTTGCTTGTGCACAAATGAttt                        >  7:215707/1‑149 (MQ=255)
                                                                                                                         gTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgtg                     <  3:106940/148‑1 (MQ=255)
                                                                                                                          taactgcttCACACACACACGAGTCTGTGTCTCGGTGACTTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTTTCTCCTTGCTTTTGCACAAATGATTtgttg                    <  8:292332/145‑3 (MQ=255)
                                                                                                                               gctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGc               >  1:173631/1‑148 (MQ=255)
                                                                                                                                ctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCGTTGCTACAGCCGGTGAGCATGTACAACAGGCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCg              <  1:217296/148‑1 (MQ=255)
                                                                                                                                   cacaCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtg          >  6:332018/1‑149 (MQ=255)
                                                                                                                                      acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGa       <  5:332018/149‑1 (MQ=255)
                                                                                                                                       ggtccccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAg      >  4:17835/5‑149 (MQ=255)
                                                                                                                                        ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc     >  7:202981/1‑149 (MQ=255)
                                                                                                                                        ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc     >  5:63241/1‑149 (MQ=255)
                                                                                                                                        ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc     <  8:215699/149‑1 (MQ=255)
                                                                                                                                         tccccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg    >  1:333480/3‑149 (MQ=255)
                                                                                                                                          acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGt   >  7:69922/1‑149 (MQ=255)
                                                                                                                                           cccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTa  >  7:287281/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTA  >  NC_002947/5902801‑5903088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑