Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,902,952 T→C 8.6% T29A (ACA→GCA)  PP_5175 ← HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,902,9520TC 8.6% 191.1 / 7.2 70T29A (ACA→GCA) PP_5175HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base C (4/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

TCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGG  >  NC_002947/5902804‑5903096
                                                                                                                                                    |                                                                                                                                                
tCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAgtgt                                                                                                                                                  <  5:67963/149‑1 (MQ=255)
   cccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGtctc                                                                                                                                               <  6:87186/149‑1 (MQ=255)
   ccaaCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCACCGAAATCGGTGGGTTGTACCTGCAGCACACCCACCCGAGGCACTGtctc                                                                                                                                               >  3:304456/4‑149 (MQ=255)
    ccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                              >  7:119485/1‑149 (MQ=255)
    ccACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                              <  3:303834/149‑1 (MQ=255)
           gACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggtg                                                                                                                                          <  3:200990/146‑3 (MQ=255)
           gACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggtg                                                                                                                                          >  4:200990/1‑144 (MQ=255)
            aCGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAATTCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACctc                                                                                                                                      <  1:258183/149‑1 (MQ=255)
              gCTACTCGAAATGTTCTTGCCGCCGACGCTGAATGACAAATCGGTCTGCACGCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGGGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACctct                                                                                                                                     <  7:154814/148‑1 (MQ=255)
                tACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTcc                                                                                                                                  >  7:91567/1‑149 (MQ=255)
                 aCGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCt                                                                                                                                 >  4:124074/1‑149 (MQ=255)
                 aCGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCt                                                                                                                                 >  6:212195/1‑149 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                               <  4:320158/149‑1 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                           <  1:37187/137‑2 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                           >  2:37187/1‑136 (MQ=255)
                    cgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGc                                                                                                                              <  5:212195/149‑1 (MQ=255)
                    cgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGc                                                                                                                              <  6:1031/149‑1 (MQ=255)
                      aaaTGATCTTGCCGCCGACGCGGTATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCgggg                                                                                                                                           >  1:77557/1‑134 (MQ=255)
                      aaaTGATCTTGCCGCCGACGCGGTATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCgggg                                                                                                                                           <  2:77557/134‑1 (MQ=255)
                       aaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                            >  5:307444/1‑148 (MQ=255)
                            tCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCg                                                                                                                      >  7:191579/1‑149 (MQ=255)
                               tGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGt                                                                                                                    >  2:217296/1‑148 (MQ=255)
                                gccgccGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTg                                                                                                                   <  1:294167/148‑1 (MQ=255)
                                 ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGa                                                                                                                  <  6:129641/148‑1 (MQ=255)
                                 ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                                 <  2:96811/149‑1 (MQ=255)
                                 ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                                 <  2:3131/149‑1 (MQ=255)
                                         gcgGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTac                                                                                                          >  6:59904/1‑148 (MQ=255)
                                           ggAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                        >  8:37349/1‑148 (MQ=255)
                                           ggAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                        <  7:37349/148‑1 (MQ=255)
                                            gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaaca                                                                                                      <  5:40364/149‑1 (MQ=255)
                                              aTGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGc                                                                                                    <  8:91567/149‑1 (MQ=255)
                                              aTGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGc                                                                                                    <  2:17200/149‑1 (MQ=255)
                                                  cAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCa                                                                                                <  8:191579/149‑1 (MQ=255)
                                                   aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACCCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAg                                                                                               >  7:239555/1‑149 (MQ=255)
                                                   aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTAc                                                                                                                           <  6:260539/121‑1 (MQ=255)
                                                   aaaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTAc                                                                                                                           >  5:260539/1‑121 (MQ=255)
                                                    aaTCGGTCTGCACCCGCGCCTGCACGTCGCCGGACAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                              <  7:131651/149‑1 (MQ=255)
                                                      tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                                 <  1:132348/128‑1 (MQ=255)
                                                      tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAg                                                                                                                 >  2:132348/1‑128 (MQ=255)
                                                              cACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAc                                                                                      <  6:307444/147‑1 (MQ=255)
                                                                 ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGAACTCTTCCTTGCTACAGCCGCTGAGCATGTACAACATCCCCAGGCAGACAACCGACa                                                                                 >  7:292332/1‑149 (MQ=255)
                                                                  cgcgCCTGCACGTGGCCTGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCAGACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAg                                                                                <  4:304456/149‑1 (MQ=255)
                                                                     gcCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGa                                                                                          <  1:211054/136‑1 (MQ=255)
                                                                     gcCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGa                                                                                          >  2:211054/1‑136 (MQ=255)
                                                                           aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                       >  4:106940/1‑149 (MQ=255)
                                                                             gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATc                                                                      >  7:215699/1‑148 (MQ=255)
                                                                                     tCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATg                                                             >  6:33245/1‑149 (MQ=255)
                                                                                         aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                         <  3:124074/149‑1 (MQ=255)
                                                                                          gTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                        <  8:239555/149‑1 (MQ=255)
                                                                                          gTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                        <  5:59904/149‑1 (MQ=255)
                                                                                             aCCCTGGCTGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                              <  7:114782/108‑1 (MQ=255)
                                                                                             aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                              >  8:114782/1‑108 (MQ=255)
                                                                                             aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTc                                                      <  6:17350/148‑1 (MQ=255)
                                                                                               ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                                   <  5:33245/149‑1 (MQ=255)
                                                                                               ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                                   >  5:142332/1‑149 (MQ=255)
                                                                                                     aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                             >  5:4735/1‑149 (MQ=255)
                                                                                                       cGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgtg                                            >  6:222507/1‑148 (MQ=255)
                                                                                                                  ggTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGAttt                                <  8:119485/149‑1 (MQ=255)
                                                                                                                  ggTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGATACAGCCGGTGAGCATGTACAAAAGACCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGATGGCTCCTTGCTTGTGCACAAATGAttt                                >  7:215707/1‑149 (MQ=255)
                                                                                                                      gTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgtg                             <  3:106940/148‑1 (MQ=255)
                                                                                                                       taactgcttCACACACACACGAGTCTGTGTCTCGGTGACTTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTTTCTCCTTGCTTTTGCACAAATGATTtgttg                            <  8:292332/145‑3 (MQ=255)
                                                                                                                            gctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGc                       >  1:173631/1‑148 (MQ=255)
                                                                                                                             ctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCGTTGCTACAGCCGGTGAGCATGTACAACAGGCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCg                      <  1:217296/148‑1 (MQ=255)
                                                                                                                                cacaCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtg                  >  6:332018/1‑149 (MQ=255)
                                                                                                                                   acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGa               <  5:332018/149‑1 (MQ=255)
                                                                                                                                    ggtccccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAg              >  4:17835/5‑149 (MQ=255)
                                                                                                                                     ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc             <  8:215699/149‑1 (MQ=255)
                                                                                                                                     ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc             >  5:63241/1‑149 (MQ=255)
                                                                                                                                     ccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGc             >  7:202981/1‑149 (MQ=255)
                                                                                                                                      tccccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg            >  1:333480/3‑149 (MQ=255)
                                                                                                                                       acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGt           >  7:69922/1‑149 (MQ=255)
                                                                                                                                        cccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTa          >  7:287281/1‑149 (MQ=255)
                                                                                                                                                aGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAggg  >  3:111607/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGG  >  NC_002947/5902804‑5903096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑