Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,920,077 A→T 6.1% E221D (GAA→GAT)  PP_5190 → type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,920,0770AT 6.1% 192.3 / 3.1 65E221D (GAA→GAT) PP_5190type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base T (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

AGTCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCATGGACGTTGCAGAGAA  >  NC_002947/5919929‑5920211
                                                                                                                                                    |                                                                                                                                      
agTCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGaa                                                                                                                                        <  2:236935/149‑1 (MQ=255)
agTCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACACGCCAGCGATATCCACCTGGaa                                                                                                                                        <  7:238730/149‑1 (MQ=255)
agTCGGCGAGACTGGGGGAACTTGAGCAATTGCTTGAGCTGGGAAAACGCCAGTCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCTAGCAACGCGCCAGCGATATCCACCTGGaa                                                                                                                                        <  6:343879/149‑1 (MQ=255)
 gTCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGTAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAAc                                                                                                                                       <  7:93743/149‑1 (MQ=255)
  tCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAAc                                                                                                                                       >  4:67277/1‑148 (MQ=255)
     gCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCAcg                                                                                                                                   >  2:117524/1‑149 (MQ=255)
              ggggAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAAc                                                                                                                           <  4:154495/148‑1 (MQ=255)
                ggAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAgg                                                                                                                        <  5:14572/149‑1 (MQ=255)
                     ttGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCTAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGccct                                                                                                                   >  7:112677/1‑147 (MQ=255)
                     ttGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAg                                                                                                                   >  8:263065/1‑149 (MQ=255)
                       gAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCt                                                                                                                 >  5:287108/1‑149 (MQ=255)
                        aGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTg                                                                                                                >  6:310618/1‑149 (MQ=255)
                          cAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTgcg                                                                                                              <  6:199492/149‑1 (MQ=255)
                                 ttGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCg                                                                                                        >  1:324554/1‑148 (MQ=255)
                                 ttGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCg                                                                                                        >  1:319591/1‑148 (MQ=255)
                                 ttGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCg                                                                                                        >  3:28751/1‑148 (MQ=255)
                                    aGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTAt                                                                                                     <  8:249667/148‑1 (MQ=255)
                                      cTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGa                                                                                                  >  2:114552/1‑149 (MQ=255)
                                       tGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGAt                                                                                                 >  3:108223/1‑149 (MQ=255)
                                         ggCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATc                                                                                                    <  4:246349/144‑1 (MQ=255)
                                         ggCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCgcgat                                                                                               >  3:246349/1‑145 (MQ=255)
                                           cAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGcc                                                                                             <  6:287108/149‑1 (MQ=255)
                                            aaaCGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCct                                                                                            >  5:163282/1‑149 (MQ=255)
                                               cGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCTTATCGTCGACTGGCTGTTGGAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTCGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCctgct                                                                                         <  8:66583/149‑1 (MQ=255)
                                               cGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCctgct                                                                                         <  3:312675/149‑1 (MQ=255)
                                                    ggCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCAAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGcaca                                                                                    >  6:342899/1‑149 (MQ=255)
                                                           gCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTa                                                                             <  7:141086/149‑1 (MQ=255)
                                                            ccAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTAc                                                                            <  4:28751/149‑1 (MQ=255)
                                                            ccAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTAc                                                                            <  5:200556/149‑1 (MQ=255)
                                                             cAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACg                                                                           >  3:48845/1‑149 (MQ=255)
                                                               gcgcAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGcc                                                                         <  2:335741/149‑1 (MQ=255)
                                                                    gacgacGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCCCACCGTCTACGCCTTccc                                                                    >  5:276991/1‑149 (MQ=255)
                                                                     acgacgCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTcccc                                                                   >  3:273641/1‑149 (MQ=255)
                                                                     acgacgCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTcccc                                                                   <  7:263065/149‑1 (MQ=255)
                                                                          gcgcATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCgg                                                              >  1:185184/1‑149 (MQ=255)
                                                                                 tcgtgcatatcgtCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGATCCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCAcc                                                       >  3:149098/1‑149 (MQ=255)
                                                                                 tcgtgcatatcgtCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGATCCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCAcc                                                       >  3:154127/1‑149 (MQ=255)
                                                                                 tcgtgcatatcgtCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCAcc                                                       >  6:266118/1‑149 (MQ=255)
                                                                                        tatcgtCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGt                                                                                <  8:135701/117‑1 (MQ=255)
                                                                                        tatcgtCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGt                                                                                >  7:135701/1‑117 (MQ=255)
                                                                                        tatcgtCGACTGGCTGTTGCAGTACGCCAACGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCTCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCAGGGTGACCCCGGcgg                                                <  6:92331/149‑3 (MQ=255)
                                                                                          tcgtcgACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTc                                              >  5:287/1‑149 (MQ=255)
                                                                                          tcgtcgACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTc                                              <  5:310618/149‑1 (MQ=255)
                                                                                           cgtcgACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCg                                             >  5:306937/1‑149 (MQ=255)
                                                                                             tcgACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGt                                            >  6:171213/1‑148 (MQ=255)
                                                                                               gACTGGCTTTTTCTTTCCGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCa                                          <  1:117524/148‑1 (MQ=255)
                                                                                               gACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCa                                          <  6:306937/148‑1 (MQ=255)
                                                                                                    gCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAgccgcc                                    <  4:108223/149‑1 (MQ=255)
                                                                                                     cTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCt                                   <  8:9957/149‑1 (MQ=255)
                                                                                                     cTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACCCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCt                                   <  7:239244/149‑1 (MQ=255)
                                                                                                        ttGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGaa                                <  3:67277/149‑1 (MQ=255)
                                                                                                              tACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCt                          <  6:287/149‑1 (MQ=255)
                                                                                                                cGCCATCGAGCAACGCGCCAGCGATATCCACCTGGATCCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTgg                        <  4:149098/149‑1 (MQ=255)
                                                                                                                cGCCATCGAGCAACGCGCCAGCGATATCCACCTGGATCCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTgg                        <  4:154127/149‑1 (MQ=255)
                                                                                                                cGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTgg                        <  2:319591/149‑1 (MQ=255)
                                                                                                                cGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTgg                        <  2:324554/149‑1 (MQ=255)
                                                                                                                cGCCATCAAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTgg                        <  5:342899/149‑1 (MQ=255)
                                                                                                                 gCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTggg                       <  4:48845/149‑1 (MQ=255)
                                                                                                                 gCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCACCGCCGGGGTCa                                                         >  8:280068/1‑115 (MQ=255)
                                                                                                                   cATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGcc                     >  5:199020/1‑149 (MQ=255)
                                                                                                                   cATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGcc                     >  7:158515/1‑149 (MQ=255)
                                                                                                                     tCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGc                   <  2:185184/149‑1 (MQ=255)
                                                                                                                        aGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCa                  <  6:179228/147‑1 (MQ=255)
                                                                                                                        aGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCa                  >  5:179228/1‑147 (MQ=255)
                                                                                                                          cAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCATGGa              >  8:205041/1‑149 (MQ=255)
                                                                                                                           aaCGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCATGGAc             >  6:238546/1‑149 (MQ=255)
                                                                                                                               agtcgacGATATGCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCATGGACGTTg         <  5:214454/143‑1 (MQ=255)
                                                                                                                                      gaactgcACCTGGAACCACGCCGCGATCAGGGCCAGCTGCGCTACCGTCTCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGGCAGCCGCCTGAATCACCTGGGCCGCATGGACGTTGCAGAGaa  <  8:112677/143‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
AGTCGGCGAGCCTGGGGGAACTTGAGCAATTGCTTGAGCTGGGCAAACGCCAGGCCGAGGCCAGCGCAGACGACGCGCATATCGTGCATATCGTCGACTGGCTGTTGCAGTACGCCATCGAGCAACGCGCCAGCGATATCCACCTGGAACCACGCCGCGAACAGGGCCAGCTGCGCTACCGTATCGATGGCCTGCTGCACACCGTCTACGCCTTCCCCGCCGGGGTCACCCTGGCCCTCGTCAGCCGCCTGAAACACCTGGGCCGCATGGACGTTGCAGAGAA  >  NC_002947/5919929‑5920211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑