Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,949,697	G→T	6.9%	K138N (AAG→AAT)	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,949,697	0	G	T	6.9%	216.2 / 3.3	67	K138N (AAG→AAT)	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base T (2/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTTGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTA  >  NC_002947/5949550‑5949843                                                                                                                                                    |                                                                                                                                                    cGCCTTGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAgc                                                                                                                                                    <  3:280751/149‑1 (MQ=255) cGCCTTGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAgc                                                                                                                                                    >  1:141023/1‑149 (MQ=255)   ggttGCGCGCTTCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAgcgc                                                                                                                                                  >  5:270971/3‑149 (MQ=255)   ccTTGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAgcgc                                                                                                                                                  >  7:149842/1‑149 (MQ=255)    gttGCGCGCTTCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGcc                                                                                                                                                 >  8:73882/2‑149 (MQ=255)     ttGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCt                                                                                                                                                >  6:227359/1‑149 (MQ=255)       gcgcGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCtgg                                                                                                                                              >  5:240280/1‑149 (MQ=255)       gcgcGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCtg                                                                                                                                               <  6:201710/148‑1 (MQ=255)         gcgAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAATCGCCtggtg                                                                                                                                            >  4:319016/1‑149 (MQ=255)         gcgAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAATCGCCtggtg                                                                                                                                            >  4:313908/1‑149 (MQ=255)         gcgAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCtggtg                                                                                                                                            >  2:49163/1‑149 (MQ=255)           gAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCTCCTGGTGGc                                                                                                                                          <  2:130234/149‑1 (MQ=255)           gAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGc                                                                                                                                          <  6:185924/149‑1 (MQ=255)                 cgcAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACAt                                                                                                                                    <  2:141023/149‑1 (MQ=255)                        gTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATcggcggc                                                                                                                             >  3:28124/1‑149 (MQ=255)                         tAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCCCCCGGTGGAGGTCATCTCTGGCCGTCAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCAGGTGGCCGACATcggcggcg                                                                                                                            >  5:50965/1‑149 (MQ=255)                         tAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCAGGCAAGCGCCTGGTGGCCGACATcggcggcg                                                                                                                            >  8:221486/1‑149 (MQ=255)                           aCGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGAGGAGGTCATATCTGGCCGTGAAGAGGCGCCCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCCAGCGCCTGGTGGCCGACATcggcgtccg                                                                                                                           >  7:69251/1‑144 (MQ=255)                                   aTCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGGGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCCCACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACAGCGGCGGCGG‑GAGTACCGa                                                                                                                 <  4:64171/149‑1 (MQ=255)                                    tCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGaa                                                                                                                <  6:174041/149‑1 (MQ=255)                                         cgcgcCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATtca                                                                                                            >  4:4900/1‑148 (MQ=255)                                           cgcCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAATCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATtcatca                                                                                                         <  3:313908/149‑1 (MQ=255)                                           cgcCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAATCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATtcatca                                                                                                         <  3:319016/149‑1 (MQ=255)                                            gcCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTcatcat                                                                                                        <  4:211226/149‑1 (MQ=255)                                             ccGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCAtt                                                                                                       <  6:240280/149‑1 (MQ=255)                                              cGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTg                                                                                                      <  7:73882/149‑1 (MQ=255)                                              cGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTg                                                                                                      >  2:296263/1‑149 (MQ=255)                                              cGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTg                                                                                                      <  5:227359/149‑1 (MQ=255)                                              cGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTg                                                                                                      >  2:89515/1‑149 (MQ=255)                                                 aGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGcc                                                                                                   <  1:116557/149‑1 (MQ=255)                                                 aGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGc                                                                                                    >  8:314969/1‑148 (MQ=255)                                                       ccTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCCGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCt                                                                                             >  5:148358/1‑149 (MQ=255)                                                          cGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACGCCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCAtt                                                                                                       <  6:260508/136‑1 (MQ=255)                                                          cGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACGCCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCAtt                                                                                                       >  5:260508/1‑136 (MQ=255)                                                           ggCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTc                                                                                           <  4:28124/147‑1 (MQ=255)                                                            gCCACCCGGTGGAGGTCATCTCTGGCCGTGATGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAATCGGCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAg                                                                                        <  6:50965/149‑1 (MQ=255)                                                            gCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAg                                                                                        <  8:149842/149‑1 (MQ=255)                                                                cccGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCgc                                                                                    <  3:4900/149‑1 (MQ=255)                                                                   cgTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACGCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGaa                                                                                                                <  8:5469/117‑1 (MQ=255)                                                                       gAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTgcg                                                                             <  7:314969/149‑1 (MQ=255)                                                                        aGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTgcgc                                                                            <  1:296263/149‑1 (MQ=255)                                                                        aGGTCAGCTCTGGCAGTGAAGAGGCGCGGCTGATCTACCTGGGCGGGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGAGATCGGCGGCGG‑CAGTAGCGAATTCATCATTGGCCAGCGCTTGGAGCCGCTGCTgcgc                                                                            <  8:69251/149‑1 (MQ=255)                                                                                    gCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGGCCAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCt                                                                    <  1:89515/146‑1 (MQ=255)                                                                                    gCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTg                                                                   <  7:221486/146‑1 (MQ=255)                                                                                    gCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTg                                                                   >  1:226927/1‑146 (MQ=255)                                                                                    gCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTg                                                                   <  2:226927/146‑1 (MQ=255)                                                                                        tGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGc                                                                      <  3:107011/139‑1 (MQ=255)                                                                                        tGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGc                                                                      >  4:107011/1‑139 (MQ=255)                                                                                        tGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATgg                                                            <  8:257994/149‑1 (MQ=255)                                                                                        tGAAGAGGCGCGCCCGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATgg                                                            <  6:148358/149‑1 (MQ=255)                                                                                             agGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGc                                                       <  3:3974/149‑1 (MQ=255)                                                                                                 gcgcCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGt                                                     >  4:13854/1‑147 (MQ=255)                                                                                                 gcgcCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGt                                                     >  3:81091/1‑147 (MQ=255)                                                                                                           tACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTAcc                                           >  7:61825/1‑147 (MQ=255)                                                                                                              cTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAg                                                  <  5:87154/137‑1 (MQ=255)                                                                                                              cTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAg                                                  >  6:87154/1‑137 (MQ=255)                                                                                                                     tgtCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTc                               <  6:270971/149‑1 (MQ=255)                                                                                                                         gCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCcgcg                           >  4:324175/1‑149 (MQ=255)                                                                                                                         gCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTAACGAATTCATCATTGGCCAGCGCGTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGCGCTTTCCCCAGCGCTAGTTCcgc                            >  7:304120/1‑148 (MQ=255)                                                                                                                          cacaCCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGa                          >  5:62159/1‑149 (MQ=255)                                                                                                                               ccTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGc                                                       <  2:137175/115‑1 (MQ=255)                                                                                                                               ccTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGc                                                       >  1:137175/1‑115 (MQ=255)                                                                                                                                   gCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGa                  <  1:49163/148‑1 (MQ=255)                                                                                                                                   gCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGa                  >  4:23936/1‑148 (MQ=255)                                                                                                                                      gACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCAc              >  1:71686/1‑149 (MQ=255)                                                                                                                                       acacacCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCAcc             <  2:241070/149‑1 (MQ=255)                                                                                                                                          cacCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCg          <  3:13854/149‑1 (MQ=255)                                                                                                                                             ccGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGcc       <  3:324175/149‑1 (MQ=255)                                                                                                                                              cGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGccc      >  5:142082/1‑149 (MQ=255)                                                                                                                                               ggCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCg     >  5:22149/1‑149 (MQ=255)                                                                                                                                                  aaGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTa  >  8:69201/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGCCTTGCGCGAAGCGCGCAACCGTAACGAATTCATCCAGCGCGCCGAAGCCATCCTCGGCCACCCGGTGGAGGTCATCTCTGGCCGTGAAGAGGCGCGCCTGATCTACCTGGGCGTGTCGCACACCCTGGCCGACACACCCGGCAAGCGCCTGGTGGCCGACATCGGCGGCGG‑CAGTACCGAATTCATCATTGGCCAGCGCTTCGAGCCGCTGCTGCGCGAAAGCCTGCAGATGGGTTGCGTGAGCTTTACCCAGCGCTATTTCCGCGACGGCAAGATCACCCCGGCCCGCTA  >  NC_002947/5949550‑5949843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑