Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,950,984 C→A 8.0% R658L (CGT→CTT)  ppk ← polyphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,950,9840CA 8.0% 140.8 / 4.1 50R658L (CGT→CTT) ppkpolyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-02

CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCC  >  NC_002947/5950839‑5951123
                                                                                                                                                  |                                                                                                                                           
cTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTAGTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGc                                                                                                                                           <  7:216538/148‑1 (MQ=255)
   gTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑GCAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGt                                                                                                                                       >  8:76727/1‑149 (MQ=255)
   gTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCCGGAAGCAGGTCTCGACACGCTTg                                                                                                                                        >  2:244536/1‑148 (MQ=255)
       ccGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGa                                                                                                                                    <  5:287521/148‑1 (MQ=255)
       ccGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTACAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGGTCCTTCTTCCCCCGCAGCA‑ACAGTTTCTTGCCTTCCCCCGGGAAGCAGGTCTCGACACGCTTGTCGAg                                                                                                                                   >  6:242506/1‑149 (MQ=255)
        cGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAgg                                                                                                                                  <  5:139199/149‑1 (MQ=255)
        cGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAga                                                                                                                                  <  1:77006/149‑2 (MQ=255)
                 tgctgcGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑GCAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtcc                                                                                                                         <  7:76727/149‑1 (MQ=255)
                     gcgcACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatc                                                                                                                      >  8:86626/1‑148 (MQ=255)
                      cgcACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatc                                                                                                                      <  4:71736/147‑1 (MQ=255)
                             aGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGATAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGgcg                                                                                                             >  4:695/1‑149 (MQ=255)
                              gcgcCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGgcgc                                                                                                            <  4:117315/149‑1 (MQ=255)
                               cgcCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGTGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGgcgc                                                                                                            <  6:156892/148‑1 (MQ=255)
                               cgcCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCt                                                                                                           >  6:71722/1‑149 (MQ=255)
                                        ggCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTGTTCACCCGCAGGA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGt                                                                                                   <  6:30509/148‑1 (MQ=255)
                                         gCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGt                                                                                                   <  1:244536/147‑1 (MQ=255)
                                         gCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGt                                                                                                   <  5:71722/147‑1 (MQ=255)
                                           tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGa                                                                                                >  1:2770/1‑148 (MQ=255)
                                           tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGa                                                                                                >  7:91943/1‑148 (MQ=255)
                                           tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgacct                                                                                                                               >  3:327055/1‑113 (MQ=255)
                                           tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgacct                                                                                                                               <  4:327055/117‑5 (MQ=255)
                                                 gTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTAGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGctc                                                                                         <  3:73557/149‑1 (MQ=255)
                                                 gTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTATTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGc                                                                                           >  5:279042/1‑147 (MQ=255)
                                                 gTCCAGGAGTGGGTATTGTCTGTCAGTTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTTCCTTCCACCGGGAAGCAGGTCTCGACACGATTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGc                                                                                           <  6:279042/147‑1 (MQ=255)
                                                   ccAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCtt                                                                                       >  1:247621/1‑149 (MQ=255)
                                                        cGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgccg                                                                                  <  7:86626/149‑1 (MQ=255)
                                                         gTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgccgc                                                                                 >  5:230855/1‑149 (MQ=255)
                                                              tATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTg                                                                            <  2:2770/149‑1 (MQ=255)
                                                                  gTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAgg                                                                         <  2:247621/148‑1 (MQ=255)
                                                                             aGCCTTCCAGCTCCTTCTTCACCCGCAGCATACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGaa                                                                 >  1:31333/1‑146 (MQ=255)
                                                                             aGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGaa                                                                 <  2:31333/145‑1 (MQ=255)
                                                                                 ttCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCgg                                                                                                               >  8:129882/1‑95 (MQ=255)
                                                                                 ttCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCgg                                                                                                               <  7:129882/95‑1 (MQ=255)
                                                                                        tCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTc                                                  >  1:227830/1‑149 (MQ=255)
                                                                                         ccTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTc                                                  >  8:150590/1‑148 (MQ=255)
                                                                                             cttcACCAGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAg                                               <  7:80496/147‑1 (MQ=255)
                                                                                                     gcagca‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGcc                                      >  8:297251/1‑148 (MQ=255)
                                                                                                          a‑aCAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGAt                                >  5:191690/1‑149 (MQ=255)
                                                                                                             cAGTTGCTTGCCTTCCACCGCTAAGCATGTCTCGACACGCTTGTCGAGGTTGCGCTACATCCCGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCg                              <  8:134797/149‑1 (MQ=255)
                                                                                                              aGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATc                               >  1:56090/1‑147 (MQ=255)
                                                                                                              aGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGa                             <  6:230855/149‑1 (MQ=255)
                                                                                                               gTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAg                            <  8:91943/149‑1 (MQ=255)
                                                                                                                         ttCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGa                  >  8:224145/1‑149 (MQ=255)
                                                                                                                           ccACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAt                 <  3:695/148‑1 (MQ=255)
                                                                                                                            cACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgt               >  8:64101/1‑149 (MQ=255)
                                                                                                                               cGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttgt            <  6:191690/149‑1 (MQ=255)
                                                                                                                               cGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttgt            <  8:34098/149‑1 (MQ=255)
                                                                                                                                        aacctcGACAAGCGTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAccc   >  6:296127/4‑149 (MQ=255)
                                                                                                                                            tcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAcccc  <  2:107556/146‑1 (MQ=255)
                                                                                                                                            tcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAcccc  >  1:107556/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCA‑ACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCC  >  NC_002947/5950839‑5951123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑