Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,989,221 A→T 6.1% intergenic (+515/‑31) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,989,2210AT 6.1% 195.6 / 3.0 66intergenic (+515/‑31)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base T (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

AGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACGGGTG  >  NC_002947/5989084‑5989363
                                                                                                                                          |                                                                                                                                              
aGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCgg                                                                                                                                        <  1:176237/146‑1 (MQ=255)
  cTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGtgtg                                                                                                                                   <  4:239267/149‑1 (MQ=255)
     ggTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGTGTGTgg                                                                                                                                  <  5:295146/147‑1 (MQ=255)
            cTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGa                                                                                                                         >  7:232666/1‑149 (MQ=255)
             ttCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGAt                                                                                                                        >  2:310252/1‑149 (MQ=255)
               ccccGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGAt                                                                                                                        <  6:127634/147‑1 (MQ=255)
                 ccGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGtga                                                                                                                    >  7:264650/1‑149 (MQ=255)
                       aCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑AGCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAgg                                                                                                              >  1:239120/1‑149 (MQ=255)
                       aCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGGTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAgg                                                                                                              <  8:297291/149‑1 (MQ=255)
                               cTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGt                                                                                                      >  7:245932/1‑149 (MQ=255)
                                   gTTCACACCACACCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGCGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATg                                                                                                  <  4:249294/149‑1 (MQ=255)
                                       acacCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAg                                                                                              >  5:261790/1‑149 (MQ=255)
                                       acacCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAg                                                                                              >  6:126766/1‑149 (MQ=255)
                                         acCAATCCCAAGGAATTATCCTACACGACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGt                                                                                             >  1:177512/1‑149 (MQ=255)
                                         acCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGtt                                                                                            >  6:158807/1‑149 (MQ=255)
                                             aTCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGCTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGTCAGTTGTgc                                                                                        >  5:83263/1‑149 (MQ=255)
                                               cccAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTgcgc                                                                                      >  7:220610/1‑149 (MQ=255)
                                                 cAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGt                                                                                    <  1:310252/149‑1 (MQ=255)
                                                  aaGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGt                                                                                    >  5:163773/1‑148 (MQ=255)
                                                   aGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGa                                                                                  <  5:30660/149‑1 (MQ=255)
                                                           tCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGttt                                                                          >  2:222333/1‑149 (MQ=255)
                                                            ccTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTg                                                                         >  6:273443/1‑149 (MQ=255)
                                                            ccTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTg                                                                         <  8:264650/149‑1 (MQ=255)
                                                              tACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAggg                                                                                                             <  2:224024/111‑1 (MQ=255)
                                                              tACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGa                                                                                  >  2:156567/1‑138 (MQ=255)
                                                              tACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGa                                                                                  <  1:156567/138‑1 (MQ=255)
                                                              tACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCATAACGGCAgccc                                                                                           >  8:85678/1‑126 (MQ=255)
                                                              tACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCATAACGGCAgccc                                                                                           <  7:85678/129‑4 (MQ=255)
                                                               acac‑acCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCa                                                                                                                                                >  6:259044/1‑75 (MQ=255)
                                                               acac‑acCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCa                                                                                                                                                <  5:259044/75‑1 (MQ=255)
                                                                cac‑acCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGcc                                                                      >  6:157372/1‑148 (MQ=255)
                                                                cac‑acCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGcc                                                                      >  8:57307/1‑148 (MQ=255)
                                                                        tgtCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCt                                                              >  2:105788/1‑149 (MQ=255)
                                                                          tCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTTGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTg                                                            <  2:239120/149‑1 (MQ=255)
                                                                            gACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCg                                                                                                       <  4:280824/104‑1 (MQ=255)
                                                                            gACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCg                                                                                                       >  3:280824/1‑104 (MQ=255)
                                                                               gCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTtc                                                       >  2:195804/1‑149 (MQ=255)
                                                                                  cGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCa                                                    <  8:245932/149‑1 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCTGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGtttt                                            <  3:16643/147‑1 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCTGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGtttt                                            <  3:12126/147‑1 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCTGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGAAGTTATGGCAGTTGTGAGCGTGAGGCCGTTTGGCCTACAGGCTTGCATTCTCACCTGtttt                                            >  4:12126/1‑147 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTc                                           >  2:20402/1‑148 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTc                                           <  8:232666/148‑1 (MQ=255)
                                                                                            tCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGCTCGCTGTCGGTTCTGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGtttt                                            >  4:16643/1‑147 (MQ=255)
                                                                                              cGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCTCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATCAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGATTGGCCTACCTCCTAGCCTTCACCCCGGTTTTCCAc                                        >  6:192623/1‑149 (MQ=255)
                                                                                              cGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCa                                         <  1:222333/148‑1 (MQ=255)
                                                                                                   aaaGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCcgc                                   <  7:57307/149‑1 (MQ=255)
                                                                                                       ccAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGca                                >  2:172884/1‑148 (MQ=255)
                                                                                                         ggCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGcaca                              >  5:31177/2‑148 (MQ=255)
                                                                                                         aGCTCTCGAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGGGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACaa                             <  5:256030/149‑1 (MQ=255)
                                                                                                             ctctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTgc                         >  8:126197/1‑149 (MQ=255)
                                                                                                             ctctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTgc                         <  1:20402/149‑1 (MQ=255)
                                                                                                              tctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGcac                               >  3:206643/1‑142 (MQ=255)
                                                                                                              tctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGcac                               <  4:206643/142‑1 (MQ=255)
                                                                                                              tctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGca                                <  6:163377/141‑1 (MQ=255)
                                                                                                              tctAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGca                                >  5:163377/1‑141 (MQ=255)
                                                                                                                tAGGCTGACCAGGTCGCTGTCGGTGCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTgc                         <  8:170458/146‑1 (MQ=255)
                                                                                                                  ggCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTgccg                       <  1:195804/146‑1 (MQ=255)
                                                                                                                    cTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCtt                                                             <  6:248169/106‑1 (MQ=255)
                                                                                                                    cTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCtt                                                             >  5:248169/1‑106 (MQ=255)
                                                                                                                       aCCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCt                 <  1:105788/147‑1 (MQ=255)
                                                                                                                        ccAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCg              <  5:273443/149‑1 (MQ=255)
                                                                                                                               gCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAAc       <  2:177512/149‑1 (MQ=255)
                                                                                                                               gCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAAc       <  6:261790/149‑1 (MQ=255)
                                                                                                                                cTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACg      >  5:303939/1‑149 (MQ=255)
                                                                                                                                cTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACg      >  4:168607/1‑149 (MQ=255)
                                                                                                                                  gTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACggg    >  3:265002/1‑149 (MQ=255)
                                                                                                                                    cGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACGggtg  <  7:292632/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
AGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACAC‑ACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCCGGATGTGATGAAGGGCTGCCGTTATGGCAGTTGTGCGCGTGAGGCCGTTTGGCCTACCGGCTTGCCTTCTCACCGGTTTTCCACCCCGCGCACAACTGCCGCCTTCTGCGTGGAAACGGGTG  >  NC_002947/5989084‑5989363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑