Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,011,489 T→G 12.5% I114S (ATC→AGC)  rep → single‑stranded DNA dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,011,4890TG12.5% 100.6 / 6.1 39I114S (ATC→AGC) repsingle‑stranded DNA dependent ATPase
Reads supporting (aligned to +/- strand):  ref base T (17/17);  new base G (3/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACC  >  NC_002947/6011349‑6011637
                                                                                                                                            |                                                                                                                                                    
ggTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGt                                                                                                                                              >  3:20874/1‑149 (MQ=255)
  tCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTg                                                                                                                                            <  4:212742/149‑1 (MQ=255)
         cTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCg                                                                                                                                      >  7:37635/1‑148 (MQ=255)
          ttGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGa                                                                                                                                    >  1:52867/1‑149 (MQ=255)
                cgcCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCGGActcgt                                                                                                                               <  7:148093/148‑5 (MQ=255)
                 gcCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                             <  6:314144/149‑1 (MQ=255)
                   ccGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                             >  1:303518/1‑147 (MQ=255)
                      ggggAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCCCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGaa                                                                                                                        <  5:30810/149‑1 (MQ=255)
                       gggAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAg                                                                                                                       <  1:331457/149‑1 (MQ=255)
                              ccGTGGCCTGACCGTGTGTACGTTCCACAACCTCGGACTGAACATAATCCGCAAGGAACACGAGCGCATGGGCTATAAGCAGGGTTTCTCGATCTTCGACGAGTACGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACt                                                                                                                >  5:18501/1‑149 (MQ=255)
                                gTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAAGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTcc                                                                                                              >  5:86801/1‑149 (MQ=255)
                                    ccTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGc                                                                                                           >  3:61732/1‑148 (MQ=255)
                                         ccGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCgacgac                                                                                                     <  2:99962/149‑1 (MQ=255)
                                          cGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGacgacg                                                                                                    <  4:61732/149‑1 (MQ=255)
                                                   gTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgacg                                                                                           <  8:37635/149‑1 (MQ=255)
                                                                   cTGAACATCATCCGCAAAGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGAt                                                                           <  6:86801/149‑1 (MQ=255)
                                                                         atcatcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGAca                                                                                                                                  <  6:324789/88‑1 (MQ=255)
                                                                          tcatcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGcc                                                                    >  6:173209/1‑149 (MQ=255)
                                                                            atcCTCAAGGAACACGAGCGCCTGGGCTATACGCCGGGTTTCTCGATGTTCGACGAGTACGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGTAATACTCCGGCTACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                   <  6:18501/148‑1 (MQ=255)
                                                                              ccGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTgg                                                                 >  4:52564/1‑148 (MQ=255)
                                                                                  ctggAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaagaa                                                            <  3:172700/147‑1 (MQ=255)
                                                                                   aGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAAc                                                           >  3:191507/1‑149 (MQ=255)
                                                                                     gAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaagaa                                                            >  4:172700/1‑146 (MQ=255)
                                                                                          cGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTgg                                                    >  7:239909/1‑149 (MQ=255)
                                                                                            aGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTc                                                  <  8:239909/149‑1 (MQ=255)
                                                                                                                 gTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCTACGAGATCAAGAACATGATc                                                                          <  2:327225/104‑1 (MQ=255)
                                                                                                                 gTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCAACGAGATCAAGAACATGATc                                                                          >  1:327225/1‑104 (MQ=255)
                                                                                                                     ctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                   >  3:72459/1‑107 (MQ=255)
                                                                                                                     ctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                   <  4:72459/107‑1 (MQ=255)
                                                                                                                        gATCATCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGCCGACGACCGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                      <  1:243653/149‑1 (MQ=255)
                                                                                                                              cgacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                      <  5:173209/143‑1 (MQ=255)
                                                                                                                              cgacgaGGCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGACTACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                      <  4:20874/143‑1 (MQ=255)
                                                                                                                               gacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAAccc               >  1:324065/1‑149 (MQ=255)
                                                                                                                               gacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAAccc               >  3:204109/1‑149 (MQ=255)
                                                                                                                                     tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcg                       <  7:125255/135‑1 (MQ=255)
                                                                                                                                     tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcg                       >  8:125255/1‑135 (MQ=255)
                                                                                                                                     tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCagcgc           >  5:269739/1‑147 (MQ=255)
                                                                                                                                       cGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGTCGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAg        >  2:106824/1‑148 (MQ=255)
                                                                                                                                       cGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAg        >  1:248528/1‑148 (MQ=255)
                                                                                                                                            tCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAcc  >  5:321108/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACC  >  NC_002947/6011349‑6011637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑