Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,011,500 C→A 12.8% L118M (CTG→ATG)  rep → single‑stranded DNA dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,011,5000CA12.8% 99.2 / 7.5 39L118M (CTG→ATG) repsingle‑stranded DNA dependent ATPase
Reads supporting (aligned to +/- strand):  ref base C (18/16);  new base A (3/2);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

CTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACCGC  >  NC_002947/6011358‑6011639
                                                                                                                                              |                                                                                                                                           
cTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCg                                                                                                                                        >  7:37635/1‑148 (MQ=255)
 ttGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGa                                                                                                                                      >  1:52867/1‑149 (MQ=255)
       cgcCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCGGActcgt                                                                                                                                 <  7:148093/148‑5 (MQ=255)
        gcCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                               <  6:314144/149‑1 (MQ=255)
          ccGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                               >  1:303518/1‑147 (MQ=255)
             ggggAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCCCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGaa                                                                                                                          <  5:30810/149‑1 (MQ=255)
              gggAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAg                                                                                                                         <  1:331457/149‑1 (MQ=255)
                     ccGTGGCCTGACCGTGTGTACGTTCCACAACCTCGGACTGAACATAATCCGCAAGGAACACGAGCGCATGGGCTATAAGCAGGGTTTCTCGATCTTCGACGAGTACGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACt                                                                                                                  >  5:18501/1‑149 (MQ=255)
                       gTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAAGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTcc                                                                                                                >  5:86801/1‑149 (MQ=255)
                           ccTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGc                                                                                                             >  3:61732/1‑148 (MQ=255)
                                ccGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCgacgac                                                                                                       <  2:99962/149‑1 (MQ=255)
                                 cGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGacgacg                                                                                                      <  4:61732/149‑1 (MQ=255)
                                          gTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgacg                                                                                             <  8:37635/149‑1 (MQ=255)
                                                          cTGAACATCATCCGCAAAGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGAt                                                                             <  6:86801/149‑1 (MQ=255)
                                                                atcatcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGAca                                                                                                                                    <  6:324789/88‑1 (MQ=255)
                                                                 tcatcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGcc                                                                      >  6:173209/1‑149 (MQ=255)
                                                                   atcCTCAAGGAACACGAGCGCCTGGGCTATACGCCGGGTTTCTCGATGTTCGACGAGTACGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGTAATACTCCGGCTACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                     <  6:18501/148‑1 (MQ=255)
                                                                     ccGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTgg                                                                   >  4:52564/1‑148 (MQ=255)
                                                                         ctggAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaagaa                                                              <  3:172700/147‑1 (MQ=255)
                                                                          aGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAAc                                                             >  3:191507/1‑149 (MQ=255)
                                                                            gAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaagaa                                                              >  4:172700/1‑146 (MQ=255)
                                                                                 cGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTgg                                                      >  7:239909/1‑149 (MQ=255)
                                                                                   aGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTc                                                    <  8:239909/149‑1 (MQ=255)
                                                                                                        gTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCTACGAGATCAAGAACATGATc                                                                            <  2:327225/104‑1 (MQ=255)
                                                                                                        gTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCAACGAGATCAAGAACATGATc                                                                            >  1:327225/1‑104 (MQ=255)
                                                                                                            ctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                     <  4:72459/107‑1 (MQ=255)
                                                                                                            ctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCt                                                                     >  3:72459/1‑107 (MQ=255)
                                                                                                               gATCATCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGCCGACGACCGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                        <  1:243653/149‑1 (MQ=255)
                                                                                                                     cgacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                        <  5:173209/143‑1 (MQ=255)
                                                                                                                     cgacgaGGCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGACTACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                        <  4:20874/143‑1 (MQ=255)
                                                                                                                      gacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAAccc                 >  3:204109/1‑149 (MQ=255)
                                                                                                                      gacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAAccc                 >  1:324065/1‑149 (MQ=255)
                                                                                                                            tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcg                         <  7:125255/135‑1 (MQ=255)
                                                                                                                            tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcg                         >  8:125255/1‑135 (MQ=255)
                                                                                                                            tCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCagcgc             >  5:269739/1‑147 (MQ=255)
                                                                                                                              cGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGTCGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAg          >  2:106824/1‑148 (MQ=255)
                                                                                                                              cGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAg          >  1:248528/1‑148 (MQ=255)
                                                                                                                                   tCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAcc    >  5:321108/1‑149 (MQ=255)
                                                                                                                                     aaGGCGCTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGACGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAccgc  >  5:45356/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACCGC  >  NC_002947/6011358‑6011639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑