Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,040,794	T→A	9.8%	intergenic (+19/+15)	PP_5292 → / ← PP_5293	catabolite repression control protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,040,794	0	T	A	9.8%	186.6 / 7.7	68	intergenic (+19/+15)	PP_5292/PP_5293	catabolite repression control protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/36);  new base A (4/3);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGATAAACAC  >  NC_002947/6040648‑6040937                                                                                                                                                    |                                                                                                                                                ggTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCAtt                                                                                                                                                <  5:49937/148‑1 (MQ=255) ggTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTg                                                                                                                                               <  5:199861/149‑1 (MQ=255)  gTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGc                                                                                                                                              <  8:39546/149‑1 (MQ=255)  gTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGATCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGc                                                                                                                                              <  8:175497/149‑1 (MQ=255)   ttCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCa                                                                                                                                             <  4:281590/149‑1 (MQ=255)      gACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGc                                                                                                                                           <  2:104803/148‑1 (MQ=255)      gACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCAATGCAGc                                                                                                                                           <  6:290459/148‑1 (MQ=255)       aCTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCAATGCAGccc                                                                                                                                         <  4:111734/149‑1 (MQ=255)         tACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGTGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGcccc                                                                                                                                        >  5:21928/1‑148 (MQ=255)         tACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGcccc                                                                                                                                        >  8:240959/1‑148 (MQ=255)           ccAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAAt                                                                                                                                     <  1:315731/149‑1 (MQ=255)            cAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATg                                                                                                                                    <  3:284525/149‑1 (MQ=255)            cAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCAATGCAGCCCCAATg                                                                                                                                    <  2:336591/149‑1 (MQ=255)             aGATCCTCACCCGGGGCCTGCGCGGCTTCGTGCGAAAGGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCTACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAAt                                                                                                                                     <  2:159525/147‑1 (MQ=255)             aGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCAATGCAGCCCCAAt                                                                                                                                     >  1:159525/1‑147 (MQ=255)                  cTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTAt                                                                                                                               <  4:21145/148‑1 (MQ=255)                   tCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTAttt                                                                                                                             >  8:46656/1‑149 (MQ=255)                   tCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTAttt                                                                                                                             >  5:275656/1‑149 (MQ=255)                     aCCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTAttt                                                                                                                             <  8:25095/147‑1 (MQ=255)                       cccGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATc                                                                                                                         <  7:147193/149‑1 (MQ=255)                       cccGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATc                                                                                                                         <  5:182537/149‑1 (MQ=255)                              cTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGgccgc                                                                                                                   >  6:21981/1‑148 (MQ=255)                               tGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGgccgc                                                                                                                   >  5:217342/1‑147 (MQ=255)                                      cTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGatcatc                                                                                                          <  1:219696/149‑1 (MQ=255)                                      cTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGatcatc                                                                                                          >  8:44353/1‑149 (MQ=255)                                         cGTGCGGAACGCTCGCCTGCCGCGTCAGGCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCgg                                                                                                        <  7:127925/148‑1 (MQ=255)                                         cGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCgg                                                                                                        <  6:275656/148‑1 (MQ=255)                                              gcAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATTGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGc                                                                                                   >  6:193570/1‑148 (MQ=255)                                              gcAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATAATCGGGTAGc                                                                                                   >  4:121969/1‑148 (MQ=255)                                                   gCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAg                                                                                             <  6:21928/149‑1 (MQ=255)                                                                  cAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCg                                                                              >  6:119541/1‑149 (MQ=255)                                                                   aGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGc                                                                             <  7:240959/149‑1 (MQ=255)                                                                      cACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCg                                                                          >  4:55231/1‑149 (MQ=255)                                                                       aCGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGc                                                                         >  6:250585/1‑149 (MQ=255)                                                                         gCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCAc                                                                       >  8:94243/1‑149 (MQ=255)                                                                               cccAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACcgccgc                                                                 <  7:44353/149‑1 (MQ=255)                                                                               cccAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACcgccgc                                                                 >  6:160157/1‑149 (MQ=255)                                                                                ccAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGccc                                                                                     >  5:243418/1‑128 (MQ=255)                                                                                ccAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGccc                                                                                     <  6:243418/128‑1 (MQ=255)                                                                                 cAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATATAAAATGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCAATCATTCGCACGCACCGCAGCga                                                               >  8:165694/1‑149 (MQ=255)                                                                                  aGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCAccg                                                                     <  3:238267/142‑1 (MQ=255)                                                                                  aGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCAccg                                                                     <  3:249384/142‑1 (MQ=255)                                                                                  aGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCAccg                                                                     >  4:249384/1‑142 (MQ=255)                                                                                  aGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCgat                                                              >  2:313498/1‑149 (MQ=255)                                                                                  aGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACAATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCAccg                                                                     >  4:238267/1‑142 (MQ=255)                                                                                    cATGCACCGCTGATCGTCGACTATGAGCTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCgat                                                              >  1:176374/1‑148 (MQ=255)                                                                                     aTGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCgatgat                                                           <  2:176374/149‑1 (MQ=255)                                                                                           cGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCaa                                                     <  7:46656/149‑1 (MQ=255)                                                                                             cTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCAt                                                                                 <  5:87620/119‑1 (MQ=255)                                                                                             cTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCAt                                                                                 >  6:87620/1‑119 (MQ=255)                                                                                                aTCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATatca                                                <  6:217342/149‑1 (MQ=255)                                                                                                aTCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATatc                                                 <  5:160157/148‑1 (MQ=255)                                                                                                    tcgACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCAGTCGCCCGCACCTCCGCGAAGATGATCAATATCATCAc                                            >  2:263749/1‑149 (MQ=255)                                                                                                                  gACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGa                                 <  7:94243/147‑1 (MQ=255)                                                                                                                      cTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGGCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATgg                           <  1:189819/149‑1 (MQ=255)                                                                                                                         aCCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGc                          <  5:119541/147‑1 (MQ=255)                                                                                                                           cATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAAc                      <  5:2502/149‑1 (MQ=255)                                                                                                                             tCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATCGCAAACAg                    >  8:162999/1‑149 (MQ=255)                                                                                                                                 aaaGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCAc                 <  2:161987/148‑1 (MQ=255)                                                                                                                                 aaaGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCAc                 >  1:161987/1‑148 (MQ=255)                                                                                                                                    ggCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGTGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGc             <  5:193570/149‑1 (MQ=255)                                                                                                                                     gCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGcc            <  3:55231/149‑1 (MQ=255)                                                                                                                                        aTTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGccc           >  3:114061/1‑147 (MQ=255)                                                                                                                                        aTTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGccc           <  4:114061/147‑1 (MQ=255)                                                                                                                                        aTTGGGGCTGCAATGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGa         >  4:207662/1‑149 (MQ=255)                                                                                                                                         ttGGGGCTGCAATGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGa         >  7:120582/1‑148 (MQ=255)                                                                                                                                         ttGGGGCTGCAATGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGa         >  8:30913/1‑148 (MQ=255)                                                                                                                                            gggCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGATaaa     <  5:250585/149‑1 (MQ=255)                                                                                                                                               cTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGATAAacac  <  1:313498/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                GGTTCGACTACCAGATCCTCACCCCGGGCCTGCGCCGCTTCGTGCGCAACGCTCGCCTGCCGCGTCAGCCACGCTTCTCCCAGCATGCACCGCTGATCGTCGACTATGA‑CTGGACGCTGACCATCTAAAAGGCCATTGGGGCTGCATTGCAGCCCCAATGGTTATTTGATCGGCCGCCAGATCATCGGGTAGCGATAAGGCTTGCCCTCATTCGCCCGCACCGCCGCGATGATGATCAATATCATCACTGCCACCGTGAGCATGGCAAACAGCACCAGCCCGATAAACAC  >  NC_002947/6040648‑6040937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑