Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,042,888	G→T	7.5%	M275I (ATG→ATT)	PP_5295 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,042,888	0	G	T	7.5%	236.7 / 3.3	76	M275I (ATG→ATT)	PP_5295	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base T (2/4);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGACCATGAA  >  NC_002947/6042742‑6043032                                                                                                                                                   |                                                                                                                                                    ccACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATg                                                                                                                                                     >  6:301782/1‑147 (MQ=255) ccACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATg                                                                                                                                                     >  1:50453/1‑147 (MQ=255) ccACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCa                                                                                                                                                   <  7:308762/149‑1 (MQ=255) ccACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCACAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATg                                                                                                                                                     >  1:55370/1‑147 (MQ=255)  cacaGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑g                                                                                                                                                 <  3:133549/149‑1 (MQ=255)  cacaGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑g                                                                                                                                                 >  2:32987/1‑149 (MQ=255)      gcgcCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAAc                                                                                                                                             <  2:294114/149‑1 (MQ=255)          cTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGGCTGGACTTCCTGATTCA‑GGAACGCaa                                                                                                                                         >  4:151872/1‑149 (MQ=255)          cTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGCATCA‑GGAAGTcca                                                                                                                                         <  3:324537/149‑3 (MQ=255)          cTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCaa                                                                                                                                         >  2:116169/1‑149 (MQ=255)             gAGCAGTATATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGCATCA‑GGAAGTccag                                                                                                                                        <  1:284982/147‑4 (MQ=255)             gAGCAGGAGATGGTACTGCTGGCCCAGAAACGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGCATCA‑GGAAGTccag                                                                                                                                        <  1:298859/147‑4 (MQ=255)               gCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑Ccg‑c                                                                                                                                   >  2:186357/1‑148 (MQ=255)                   gagaTGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGc                                                                                                                              >  2:111028/1‑149 (MQ=255)                      aTGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCaa                                                                                                                           <  5:301782/149‑1 (MQ=255)                               ctgGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCgg                                                                                                                  <  1:32987/149‑1 (MQ=255)                                   cccAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTc                                                                                                               >  3:144810/1‑148 (MQ=255)                                    ccAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTcc                                                                                                              >  6:309173/1‑148 (MQ=255)                                    ccAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTcc                                                                                                              >  5:63932/1‑148 (MQ=255)                                              gACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCTCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGa                                                                                                   <  2:55370/149‑1 (MQ=255)                                              gACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGa                                                                                                   <  2:50453/149‑1 (MQ=255)                                               ccGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGAc                                                                                                  <  8:266204/148‑1 (MQ=255)                                               aCGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGAc                                                                                                  <  4:144810/149‑1 (MQ=255)                                               aCGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGAc                                                                                                  >  7:173316/1‑149 (MQ=255)                                                       gagGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGAcc                                                                                                 <  3:208027/142‑1 (MQ=255)                                                       gagGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGAcc                                                                                                 >  4:208027/1‑142 (MQ=255)                                                         ggAACTCGACCGCCTCAGCACCCACATCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACgcagca                                                                                        <  6:310913/149‑1 (MQ=255)                                                          gAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAacac                                                                                                                        >  7:242968/1‑116 (MQ=255)                                                          gAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAacac                                                                                                                        <  8:242968/116‑1 (MQ=255)                                                          gAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCAc                                                                                       >  1:113075/1‑149 (MQ=255)                                                          gAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCAc                                                                                       >  2:220296/1‑149 (MQ=255)                                                            aCTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCCCAGGCCCGCGCCAGGAAATACATATTCA‑GGAAATAAA‑ACC‑CGCGGCCAACACCCAACGCGACAAAGCCATCGACCCAACCAGCAccc                                                                                     >  4:4028/1‑147 (MQ=255)                                                                gACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCACCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAgc                                                                                 >  8:173009/1‑149 (MQ=255)                                                                 aCCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAgcg                                                                                <  6:102934/149‑1 (MQ=255)                                                                   cGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAgcggc                                                                              >  5:330715/1‑149 (MQ=255)                                                                     ccTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGcggcgg                                                                            <  1:186357/149‑1 (MQ=255)                                                                         aGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTc                                                                          <  6:330715/147‑1 (MQ=255)                                                                              ccACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGCCCCACGCAGCACGCAAGCGGCGGTCAACCTg                                                                    >  4:198141/1‑148 (MQ=255)                                                                                aCGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAg                                                                 >  6:139236/1‑149 (MQ=255)                                                                                aCGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCC‑CGCGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGaac                                                                 >  1:247868/1‑148 (MQ=255)                                                                                  gTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGTCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCCACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCACGCGGCGGTCAACCTGAAGGt                                                               >  5:241710/1‑149 (MQ=255)                                                                                     aCCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTAtt                                                            >  7:152842/1‑149 (MQ=255)                                                                                         aaGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATc                                                        <  3:4028/149‑1 (MQ=255)                                                                                         aaGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATc                                                        <  4:197919/149‑1 (MQ=255)                                                                                                 cgcgTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCa                                                                                               >  1:307610/1‑102 (MQ=255)                                                                                                 cgcgTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCa                                                                                               <  2:307610/102‑1 (MQ=255)                                                                                                  gcgTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTAGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATg                                               >  2:305971/1‑149 (MQ=255)                                                                                                     tGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGt                                            <  3:260184/149‑1 (MQ=255)                                                                                                      gCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTg                                                           <  3:46497/133‑1 (MQ=255)                                                                                                      gCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTACTGATGCA‑GGAACTCAA‑CCC‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACCCAGCACCCAAACGGCCGTCAACCAGAACGTATTg                                                           >  4:46497/1‑133 (MQ=255)                                                                                                       cTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCggg                                           <  2:247868/148‑3 (MQ=255)                                                                                                        tCAAGGGTGGGGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAg                                                                 <  7:149829/125‑1 (MQ=255)                                                                                                        tCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGaa                                         >  3:34554/1‑149 (MQ=255)                                                                                                        tCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGa                                          >  3:160445/1‑148 (MQ=255)                                                                                                        tCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCAAGCAAGCGGCGGTCAACCAGAAGGTATTGATAGAACAGATGCGTGa                                          >  4:108255/1‑148 (MQ=255)                                                                                                        tCAAGGGTGGAGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAg                                                                 >  8:149829/1‑125 (MQ=255)                                                                                                         cAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGGCCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGaa                                         >  8:258578/1‑148 (MQ=255)                                                                                                           aGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACaa                                      <  6:63932/149‑1 (MQ=255)                                                                                                           aGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACaa                                      <  8:173316/149‑1 (MQ=255)                                                                                                             tgTGGCGGCGCCGCTGGCCGGCGCCGTGACTTTCTGATTCA‑CAAACTCAACCCGCCTTGGCTTATAAGTATGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACaa                                      <  1:111028/148‑1 (MQ=255)                                                                                                                  cggcgCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGa                               <  5:309173/149‑1 (MQ=255)                                                                                                                   ggcgCCGCTGGCCGGCGCCTGGACTTCCTGATGCAGGGAACTCAA‑CCG‑CGCGGCCAACACCATCGGCTCCAGGGCGTTCGCCCCACGCAGCACGCAAGCGGCGGTCACCCTGAAGGTATTGAGCGAAc                                                    >  1:217210/1‑128 (MQ=255)                                                                                                                   ggcgCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAAc                                                    <  2:217210/127‑1 (MQ=255)                                                                                                                   ggcgCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGaa                              <  1:220296/149‑1 (MQ=255)                                                                                                                       ccgcTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATc                          <  3:108255/149‑1 (MQ=255)                                                                                                                       ccgcTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATc                          <  3:151872/149‑1 (MQ=255)                                                                                                                       ccgcTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATc                          >  6:148020/1‑149 (MQ=255)                                                                                                                           tGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGGCCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGt                      <  7:258578/149‑1 (MQ=255)                                                                                                                             gCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTaa                    <  4:160445/149‑1 (MQ=255)                                                                                                                                     cTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGc                 >  2:167842/1‑144 (MQ=255)                                                                                                                                     cTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGc                 <  1:167842/144‑1 (MQ=255)                                                                                                                                     cTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCAccc            >  2:338641/1‑149 (MQ=255)                                                                                                                                        gACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGa         >  7:160143/1‑149 (MQ=255)                                                                                                                                          cTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGAcc       <  2:10335/149‑1 (MQ=255)                                                                                                                                             ccTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGACCAt     >  4:2122/1‑148 (MQ=255)                                                                                                                                               tGATGCA‑GGATCTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGt                                            >  4:216602/1‑107 (MQ=255)                                                                                                                                               tGATGCA‑GGATCTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGt                                            <  3:216602/107‑1 (MQ=255)                                                                                                                                               tGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTCAAGGTATTGATAGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGACCATGaa  >  7:119041/1‑149 (MQ=255)                                                                                                                                                 aTGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGACCATGaa  <  8:152842/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACGTCACCGAAGTGCGCCGCGTGCTCAAGGGTGGCGGCGCCGCTGGCCGGCGCCTGGACTTCCTGATGCA‑GGAACTCAA‑CCG‑CGAGGCCAACACCCTCGGCTCCAAGGCCTTCGACCCACGCAGCACGCAAGCGGCGGTCAACCTGAAGGTATTGATCGAACAGATGCGTGAACAAGTACAGAACATCGAGTAAGGCCACCCCGACCATGAA  >  NC_002947/6042742‑6043032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑