Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 97,592 G→T 15.0% intergenic (+21/+15) PP_0090 → / ← PP_0091 hypothetical protein/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294797,5920GT15.0% 74.3 / 7.9 39intergenic (+21/+15)PP_0090/PP_0091hypothetical protein/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (16/14);  new base T (3/3);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

AAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCA  >  NC_002947/97450‑97739
                                                                                                                                              |                                                                                                                                                   
aaGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagcag                                                                                                                                               >  6:108005/1‑149 (MQ=255)
aaGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCAGCAGGGCCCGGGGCTGCCAaccac                                                                                                                                               >  8:242134/1‑148 (MQ=255)
        tCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCTGGGGCTGCTTCGCAGccccggg                                                                                                                                        <  6:51918/148‑4 (MQ=255)
                             gTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATGTTTCAGATGAACGATCGATAAGCATCAGGGCCCGGGGCTGGGTAGCAGGCCCAGACTCACGGGATTCAGAACACGTc                                                                                                                  >  6:329748/1‑149 (MQ=255)
                                           gctCAAGGACCATAACATGGTCGTCGGGACCTGCGATGGCGGGACGAACAACACCAACTATCACAAGAACGATCTATAACCATCAGCGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTca                                                                                                    <  6:110619/147‑1 (MQ=255)
                                            gTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTcac                                                                                                   >  6:133250/1‑149 (MQ=255)
                                             tCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGGAGCAGCCCCACACTCACGGGTTGCAGAACCCTTCGCTAGGCTCGTTcacc                                                                                                  >  7:285120/1‑149 (MQ=255)
                                               aaGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGAAAAGCATCAGGGCCCGGGGATGCCAAGCAGCCCCACACTCACGGGATGCAGAAACCGTCCCTAGGCGCGTTcaccac                                                                                                >  2:77078/1‑149 (MQ=255)
                                                          cttGGTCGTCGGGACGTGCTATGGCGGTACGACGAAGATCATCTATCAGAATAACGATCGATAAGCATCAGGGCCGGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGt                                                                                     <  1:77078/147‑1 (MQ=255)
                                                             gttggtcgGGACCTGCGATGGGGTGACTAAGAATATCATCTATCAGAAGAACGAGCTATAAGCATCAGGGCCCGGGGCTGGGATGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTATGCTCGTTCACCACCACCTTGCGGTTg                                                                                   <  7:242134/144‑1 (MQ=255)
                                                                  tcgGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTc                                                                              >  4:292868/1‑148 (MQ=255)
                                                                  tcgGGACCTGCGATGGAGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCa                                                                             >  4:297795/1‑149 (MQ=255)
                                                                     tgACCTGCTAGGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCAGCAGGGCCCGGGGCTGCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCatat                                                                          <  6:236366/148‑1 (MQ=255)
                                                                        ccTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagc                                                                       <  5:306741/149‑1 (MQ=255)
                                                                            cGATGGCTGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAgcagca                                                                   <  1:220107/149‑1 (MQ=255)
                                                                             gATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGAAAAGCATCAGGCCCCGGGGCTGCTAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCAc                                                                  <  8:206669/149‑1 (MQ=255)
                                                                                 gCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGTCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGc                                                              <  5:108005/149‑1 (MQ=255)
                                                                                    ggACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGGGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTTCg                                                             >  5:115233/1‑147 (MQ=255)
                                                                                        gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAATCAGCCCCACACTAACGGGATTAAGAACACTTCGCTAGGCTCCTTAACCACCACAATGCGGATGGCGTCATAAAGCAGCCCCTGCGGCTcc                                                        >  7:52075/1‑148 (MQ=255)
                                                                                        gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagcagc                                                                    >  4:269891/1‑136 (MQ=255)
                                                                                        gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagcagc                                                                    <  3:269891/136‑1 (MQ=255)
                                                                                        gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTcc                                                        >  7:234166/1‑148 (MQ=255)
                                                                                              gaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGc                                                              >  2:60319/1‑136 (MQ=255)
                                                                                              gaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGc                                                              <  1:60319/136‑1 (MQ=255)
                                                                                                       tCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgcg                                        >  6:276970/1‑149 (MQ=255)
                                                                                                          gaagaaCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCACCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGc                                                              <  7:156436/124‑1 (MQ=255)
                                                                                                          gaagaaCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCACCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGc                                                              >  8:156436/1‑124 (MQ=255)
                                                                                                                      atAAGCATCAGGGCCCGGGGCTGCGAAGGAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCCCGCATCCAGGCGATAgc                         >  1:21558/1‑149 (MQ=255)
                                                                                                                      atAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACATCGGTAGGCTCGTTCACCACCACCATGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCCGCGCCCGCGCGTCCACGCGATAgc                         >  2:270703/1‑149 (MQ=255)
                                                                                                                                 ggCCCGGGGCTGCGACCCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGcc              <  8:285120/149‑1 (MQ=255)
                                                                                                                                   tgtggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGc                               >  7:235847/4‑130 (MQ=255)
                                                                                                                                   tgtggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGc                               <  8:235847/127‑1 (MQ=255)
                                                                                                                                   cccGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCtt            <  1:270703/149‑1 (MQ=255)
                                                                                                                                    gtggggCTGCTTCGCAGCCCCGCACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGGCGGCCtg            >  4:207164/3‑147 (MQ=255)
                                                                                                                                    ccGTGGCTTCCAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTg           <  5:329748/149‑1 (MQ=255)
                                                                                                                                    ccGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCtt            >  2:105566/1‑148 (MQ=255)
                                                                                                                                      ggggCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGaa         <  5:133250/149‑1 (MQ=255)
                                                                                                                                       gggCTGCTTCGCAGCCCAGCACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAg        >  6:104973/1‑149 (MQ=255)
                                                                                                                                         ggggCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGtt      <  1:150151/146‑1 (MQ=255)
                                                                                                                                              gAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCa  >  2:24135/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
AAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCA  >  NC_002947/97450‑97739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑