Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	417,372	C→T	7.9%	P361L (CCG→CTG)	waaG →	lipopolysaccharide glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	417,372	0	C	T	7.9%	176.1 / 4.8	63	P361L (CCG→CTG)	waaG	lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base T (3/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

GACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCGCC  >  NC_002947/417227‑417520                                                                                                                                                   |                                                                                                                                                     gACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCg                                                                                                                                                     >  7:171252/1‑147 (MQ=255)   cAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgcagc                                                                                                                                                 <  3:229740/149‑1 (MQ=255)       ggCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTAGCGCAATGGCCTGGCATTTGCTGATAGCGCCGAAATGTACAGCATGCCgcagcagtc                                                                                                                                             >  2:237502/1‑146 (MQ=255)         ccTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtgctg                                                                                                                                           >  5:97274/1‑149 (MQ=255)          cTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtgctgc                                                                                                                                          <  2:241087/149‑1 (MQ=255)                cTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTAACTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGt                                                                                                                                    <  3:128682/149‑1 (MQ=255)                  ggACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtg                                                                                                                                              <  4:176646/137‑1 (MQ=255)                       aGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTg                                                                                                                             <  2:97642/149‑1 (MQ=255)                           gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                                                                           >  8:94515/1‑131 (MQ=255)                           gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                                                                           <  7:94515/131‑1 (MQ=255)                           gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                      <  6:315259/136‑1 (MQ=255)                           gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                      >  5:315259/1‑136 (MQ=255)                           gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                                                                                          >  8:24361/1‑148 (MQ=255)                              cGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaa                                                                                                                       <  1:262060/148‑1 (MQ=255)                               gAACAAGAGCAGCTCATCGGCTATCTGCAGCTCATGCTCGAAGACCAGCAGGCGCG‑TGCCAGCTGGTCGAGCAATGGCCTGTCATTTGCTGATAGCGCCGATCTGTACAGCATGATGCGGCAtgcttta                                                                                                                                         <  4:249624/129‑4 (MQ=255)                               gAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaaa                                                                                                                      >  6:47019/1‑148 (MQ=255)                               gAAAAAGAGCAGCTCAACGGCTATCTGCAGAGCATGCTCGAAGACCCGCAGACGCG‑TGCCAGCTGGTCGACCAATGGCCTGGCATTTGATGATAGCCCCCATCTGTACAGCAAGCTGCGGCAtgcagta                                                                                                                                         >  3:249624/1‑125 (MQ=255)                                 aCAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaaaa                                                                                                                     >  1:88685/1‑148 (MQ=255)                                      agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                              <  6:66567/149‑1 (MQ=255)                                        cagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATg                                                                                                            <  7:155669/149‑1 (MQ=255)                                          gcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGaa                                                                                                          <  1:85865/149‑1 (MQ=255)                                             cAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACATCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                                                                                        <  2:304777/148‑1 (MQ=255)                                              aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                              >  4:220562/1‑141 (MQ=255)                                              aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                              <  3:220562/141‑1 (MQ=255)                                              aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGACGCt                                                                                                       >  5:246351/1‑148 (MQ=255)                                              aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCt                                                                                                       >  6:81347/1‑148 (MQ=255)                                                 ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGata                                                                                                   >  2:311712/1‑149 (MQ=255)                                                      tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTg                                                                                                <  8:171252/147‑1 (MQ=255)                                                      tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGc                                                                                              >  4:251693/1‑149 (MQ=255)                                                         gCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCg                                                                                            >  6:163139/1‑148 (MQ=255)                                                         gCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGACATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCg                                                                                            >  1:264182/1‑148 (MQ=255)                                                          cAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTCGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGaa                                                                                          >  2:35814/1‑149 (MQ=255)                                                               cATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCtt                                                                                     <  7:273521/149‑1 (MQ=255)                                                               cATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCtt                                                                                     >  2:178546/1‑149 (MQ=255)                                                                      gAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAAc                                                                                <  5:163139/147‑1 (MQ=255)                                                                        aGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGAACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                            <  6:97274/149‑1 (MQ=255)                                                                                   cgcg‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGc                                                                 <  7:216032/149‑1 (MQ=255)                                                                                    gcg‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCg                                                                <  5:81347/149‑1 (MQ=255)                                                                                      g‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTg                                                              <  5:47019/149‑1 (MQ=255)                                                                                        tGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACCGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGa                                                             <  6:222407/149‑1 (MQ=255)                                                                                              cTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCtt                                                       <  2:264182/149‑1 (MQ=255)                                                                                                 gTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGa                                                    >  1:18753/1‑149 (MQ=255)                                                                                                     cgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGcc                                                >  3:82801/1‑149 (MQ=255)                                                                                                      gcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCg                                               >  8:148204/1‑149 (MQ=255)                                                                                                             ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                            <  4:27287/113‑1 (MQ=255)                                                                                                             ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                            >  3:27287/1‑113 (MQ=255)                                                                                                             ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgc                                        >  5:101075/1‑149 (MQ=255)                                                                                                              cTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcg                                       >  7:99450/1‑149 (MQ=255)                                                                                                               tGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcgc                                      >  1:14009/1‑149 (MQ=255)                                                                                                                 gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAATCGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcgc                                      <  1:237502/147‑1 (MQ=255)                                                                                                                          gATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                           >  2:211974/1‑149 (MQ=255)                                                                                                                           aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa                            <  1:178546/147‑1 (MQ=255)                                                                                                                           aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa                            <  3:251693/147‑1 (MQ=255)                                                                                                                           aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAgg                          <  7:24361/149‑1 (MQ=255)                                                                                                                           aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                           <  3:270670/148‑1 (MQ=255)                                                                                                                             aGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGt                         <  8:99450/148‑1 (MQ=255)                                                                                                                              gcgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTc                        >  3:168253/1‑148 (MQ=255)                                                                                                                               cgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTa                      <  2:88685/149‑1 (MQ=255)                                                                                                                                   gATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAg                                          <  4:435/125‑1 (MQ=255)                                                                                                                                   gATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAg                                          >  3:435/1‑125 (MQ=255)                                                                                                                                         tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAg               <  1:211974/146‑1 (MQ=255)                                                                                                                                         tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTg            >  1:288082/1‑149 (MQ=255)                                                                                                                                           cAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTgg           >  6:161770/1‑148 (MQ=255)                                                                                                                                                   cGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGcgcc  >  5:32409/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCGCC  >  NC_002947/417227‑417520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑