Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	417,375	A→G	8.2%	Q362R (CAG→CGG)	waaG →	lipopolysaccharide glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	417,375	0	A	G	8.2%	170.6 / 4.7	61	Q362R (CAG→CGG)	waaG	lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base G (3/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCGCCG  >  NC_002947/417229‑417521                                                                                                                                                    |                                                                                                                                                   cAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgcagc                                                                                                                                                  <  3:229740/149‑1 (MQ=255)     ggCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTAGCGCAATGGCCTGGCATTTGCTGATAGCGCCGAAATGTACAGCATGCCgcagcagtc                                                                                                                                              >  2:237502/1‑146 (MQ=255)       ccTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtgctg                                                                                                                                            >  5:97274/1‑149 (MQ=255)        cTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtgctgc                                                                                                                                           <  2:241087/149‑1 (MQ=255)              cTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTAACTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGt                                                                                                                                     <  3:128682/149‑1 (MQ=255)                ggACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAtg                                                                                                                                               <  4:176646/137‑1 (MQ=255)                     aGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTg                                                                                                                              <  2:97642/149‑1 (MQ=255)                         gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                                                                            >  8:94515/1‑131 (MQ=255)                         gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                                                                            <  7:94515/131‑1 (MQ=255)                         gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                       >  5:315259/1‑136 (MQ=255)                         gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                       <  6:315259/136‑1 (MQ=255)                         gTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                                                                                           >  8:24361/1‑148 (MQ=255)                            cGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaa                                                                                                                        <  1:262060/148‑1 (MQ=255)                             gAACAAGAGCAGCTCATCGGCTATCTGCAGCTCATGCTCGAAGACCAGCAGGCGCG‑TGCCAGCTGGTCGAGCAATGGCCTGTCATTTGCTGATAGCGCCGATCTGTACAGCATGATGCGGCAtgcttta                                                                                                                                          <  4:249624/129‑4 (MQ=255)                             gAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaaa                                                                                                                       >  6:47019/1‑148 (MQ=255)                             gAAAAAGAGCAGCTCAACGGCTATCTGCAGAGCATGCTCGAAGACCCGCAGACGCG‑TGCCAGCTGGTCGACCAATGGCCTGGCATTTGATGATAGCCCCCATCTGTACAGCAAGCTGCGGCAtgcagta                                                                                                                                          >  3:249624/1‑125 (MQ=255)                               aCAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaaaa                                                                                                                      >  1:88685/1‑148 (MQ=255)                                    agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                               <  6:66567/149‑1 (MQ=255)                                      cagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATg                                                                                                             <  7:155669/149‑1 (MQ=255)                                        gcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGaa                                                                                                           <  1:85865/149‑1 (MQ=255)                                           cAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACATCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                                                                                         <  2:304777/148‑1 (MQ=255)                                            aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                               >  4:220562/1‑141 (MQ=255)                                            aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                                                                               <  3:220562/141‑1 (MQ=255)                                            aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGACGCt                                                                                                        >  5:246351/1‑148 (MQ=255)                                            aaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCt                                                                                                        >  6:81347/1‑148 (MQ=255)                                               ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGata                                                                                                    >  2:311712/1‑149 (MQ=255)                                                    tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTg                                                                                                 <  8:171252/147‑1 (MQ=255)                                                    tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGc                                                                                               >  4:251693/1‑149 (MQ=255)                                                       gCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCg                                                                                             >  6:163139/1‑148 (MQ=255)                                                       gCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGACATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCg                                                                                             >  1:264182/1‑148 (MQ=255)                                                        cAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTCGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGaa                                                                                           >  2:35814/1‑149 (MQ=255)                                                             cATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCtt                                                                                      <  7:273521/149‑1 (MQ=255)                                                             cATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCtt                                                                                      >  2:178546/1‑149 (MQ=255)                                                                    gAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAAc                                                                                 <  5:163139/147‑1 (MQ=255)                                                                      aGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGAACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                             <  6:97274/149‑1 (MQ=255)                                                                                 cgcg‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGc                                                                  <  7:216032/149‑1 (MQ=255)                                                                                  gcg‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCg                                                                 <  5:81347/149‑1 (MQ=255)                                                                                    g‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTg                                                               <  5:47019/149‑1 (MQ=255)                                                                                      tGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACCGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGa                                                              <  6:222407/149‑1 (MQ=255)                                                                                            cTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCtt                                                        <  2:264182/149‑1 (MQ=255)                                                                                               gTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGa                                                     >  1:18753/1‑149 (MQ=255)                                                                                                   cgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGcc                                                 >  3:82801/1‑149 (MQ=255)                                                                                                    gcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCg                                                >  8:148204/1‑149 (MQ=255)                                                                                                           ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                             >  3:27287/1‑113 (MQ=255)                                                                                                           ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                             <  4:27287/113‑1 (MQ=255)                                                                                                           ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgc                                         >  5:101075/1‑149 (MQ=255)                                                                                                            cTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcg                                        >  7:99450/1‑149 (MQ=255)                                                                                                             tGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcgc                                       >  1:14009/1‑149 (MQ=255)                                                                                                               gCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAATCGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgcgc                                       <  1:237502/147‑1 (MQ=255)                                                                                                                        gATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                            >  2:211974/1‑149 (MQ=255)                                                                                                                         aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa                             <  1:178546/147‑1 (MQ=255)                                                                                                                         aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa                             <  3:251693/147‑1 (MQ=255)                                                                                                                         aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAgg                           <  7:24361/149‑1 (MQ=255)                                                                                                                         aTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                            <  3:270670/148‑1 (MQ=255)                                                                                                                           aGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGt                          <  8:99450/148‑1 (MQ=255)                                                                                                                            gcgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTc                         >  3:168253/1‑148 (MQ=255)                                                                                                                             cgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTa                       <  2:88685/149‑1 (MQ=255)                                                                                                                                 gATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAg                                           <  4:435/125‑1 (MQ=255)                                                                                                                                 gATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAg                                           >  3:435/1‑125 (MQ=255)                                                                                                                                       tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAg                <  1:211974/146‑1 (MQ=255)                                                                                                                                       tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTg             >  1:288082/1‑149 (MQ=255)                                                                                                                                         cAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTgg            >  6:161770/1‑148 (MQ=255)                                                                                                                                              gtcggcagcaTGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATcgc                   <  6:228047/132‑1 (MQ=255)                                                                                                                                              gtcggcagcaTGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATcgc                   >  5:228047/5‑136 (MQ=255)                                                                                                                                                 cGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGcgcc   >  5:32409/1‑149 (MQ=255)                                                                                                                                                  gcagcaTGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGcgccg  >  6:84446/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCG‑TGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGCGCCG  >  NC_002947/417229‑417521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑