Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	418,296	C→G	8.9%	Q27E (CAG→GAG)	PP_0345 →	heptose kinase WapP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	418,296	0	C	G	8.9%	196.7 / 7.0	65	Q27E (CAG→GAG)	PP_0345	heptose kinase WapP
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base G (3/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCA  >  NC_002947/418150‑418441                                                                                                                                                    |                                                                                                                                                   ctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTcc                                                                                                                                                    <  7:242090/147‑1 (MQ=255) ctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCa                                                                                                                                                   <  6:21664/148‑1 (MQ=255)      ctggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCg                                                                                                                                              >  6:147385/1‑148 (MQ=255)         gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAca                                                                                                                                          <  8:192242/149‑2 (MQ=255)          ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                         >  3:290001/1‑149 (MQ=255)           tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcacc                                                                                                                                        <  4:90857/149‑4 (MQ=255)           tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                        >  4:131582/1‑149 (MQ=255)           tggAGCGCAAGGCCAAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcacc                                                                                                                                        <  4:95647/149‑4 (MQ=255)            ggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt                                                                                                                                       >  8:75740/1‑149 (MQ=255)             gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                        <  1:339557/147‑1 (MQ=255)              aGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                     <  6:188866/149‑1 (MQ=255)                 gcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgaccc                                                                                                                                  <  7:294631/149‑1 (MQ=255)                  cAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccg                                                                                                                                 <  6:242419/149‑1 (MQ=255)                     ggCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcga                                                                                                                              <  5:309514/149‑1 (MQ=255)                         cAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccg                                                                                                                          >  3:304895/1‑149 (MQ=255)                             aaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCa                                                                                                                     <  3:131582/149‑1 (MQ=255)                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                           >  5:61357/1‑125 (MQ=255)                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                           <  6:61357/125‑1 (MQ=255)                                  tACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTg                                                                                                                  >  5:135498/1‑147 (MQ=255)                                       tCGCAAGCAACGCTATGGGGATGCACTCTGGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTgg                                                                                                            >  1:203827/1‑149 (MQ=255)                                       tCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGt                                                                                                           <  4:168167/149‑1 (MQ=255)                                         gcaagcaaCGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTAc                                                                                                         <  2:203827/149‑1 (MQ=255)                                           aagcaaCGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACg                                                                                                        >  6:160148/1‑148 (MQ=255)                                           aagcaaCGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGc                                                                                                       <  1:4895/149‑1 (MQ=255)                                               aaCGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATc                                                                                                    >  6:310299/1‑148 (MQ=255)                                                aCGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATc                                                                                                    >  7:201074/1‑147 (MQ=255)                                                      tGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGt                                                                                              <  4:290001/147‑1 (MQ=255)                                                           aTGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGa                                                                                       <  7:91593/149‑1 (MQ=255)                                                              cACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATc                                                                                     <  5:310299/148‑1 (MQ=255)                                                              cACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATc                                                                                     >  6:18171/1‑148 (MQ=255)                                                                         gCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGt                                                                          <  5:160148/149‑1 (MQ=255)                                                                              ttGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAgc                                                                     <  7:75740/149‑1 (MQ=255)                                                                               tGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                          >  8:75238/1‑127 (MQ=255)                                                                               tGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                          <  7:75238/127‑1 (MQ=255)                                                                                     cTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTacac                                                              <  5:147385/149‑1 (MQ=255)                                                                                      tGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGCACGGCTGACCCGCGCCCCG‑CGCAGTGAAGTGGTACGCATCGAGCGTGTCGGGTTCAACTACTTCGTCAAGCGCTACAcc                                                             >  6:38979/1‑149 (MQ=255)                                                                                         cgcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                          <  4:304895/149‑1 (MQ=255)                                                                                          gcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCgggg                                                         >  7:286509/1‑149 (MQ=255)                                                                                              gggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCgg                                                     >  8:132840/1‑149 (MQ=255)                                                                                                gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCa                                                   <  8:201074/149‑1 (MQ=255)                                                                                                 tACGAACACCTGCCGGAGGAATTCCGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACCGCTGCCCCCCGACCCG‑CTCAGTCAAGTGGTAAGCAACGACCGTGCCGTGAGCAACTACTACGTCAAGCGCTACCCCGGGGCCGGCag                                                  >  6:164037/1‑148 (MQ=255)                                                                                                  aCGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAg                                                 >  5:280112/1‑149 (MQ=255)                                                                                                    gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                  <  2:143969/146‑1 (MQ=255)                                                                                                    gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCaa                                                  >  1:143969/1‑146 (MQ=255)                                                                                                    gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGca                                               >  4:1762/1‑149 (MQ=255)                                                                                                    gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGAAGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGca                                               >  2:184969/1‑149 (MQ=255)                                                                                                    gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGCTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGca                                               >  2:60202/1‑149 (MQ=255)                                                                                                         ccTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgc                                          <  2:81424/149‑1 (MQ=255)                                                                                                                gcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAgcg                                                                    >  6:239567/1‑116 (MQ=255)                                                                                                                gcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAgcg                                                                    <  5:239567/116‑1 (MQ=255)                                                                                                                 cggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAc                                  <  6:135498/149‑1 (MQ=255)                                                                                                                     cTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTg                               >  6:339138/1‑148 (MQ=255)                                                                                                                     cTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTg                               <  5:339138/148‑1 (MQ=255)                                                                                                                      ttCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACACG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTggg                             >  1:259839/1‑149 (MQ=255)                                                                                                                         ggCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggc                           <  6:280112/148‑1 (MQ=255)                                                                                                                               cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGCGCGGCCACg                    >  2:188960/1‑149 (MQ=255)                                                                                                                                  gACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCAt                 <  5:38979/149‑1 (MQ=255)                                                                                                                                       ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg            >  2:130648/1‑149 (MQ=255)                                                                                                                                         cgtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc          >  2:63793/2‑149 (MQ=255)                                                                                                                                          gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGTCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc          >  1:232145/1‑149 (MQ=255)                                                                                                                                          gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc          >  6:344097/1‑148 (MQ=255)                                                                                                                                              gCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt      <  1:188960/148‑1 (MQ=255)                                                                                                                                              gCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt      <  5:18171/148‑1 (MQ=255)                                                                                                                                              gCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATg     >  3:108301/1‑149 (MQ=255)                                                                                                                                               cccTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATgg    >  4:2671/1‑149 (MQ=255)                                                                                                                                                  tCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCa  >  5:336329/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCT‑GATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCG‑CTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCA  >  NC_002947/418150‑418441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑