Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 425,359 A→G 8.5% intergenic (‑50/+7) PP_0349 ← / ← PP_0350 membrane protein/ferrichrome‑iron receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947425,3590AG 8.5% 161.0 / 3.6 61intergenic (‑50/+7)PP_0349/PP_0350membrane protein/ferrichrome‑iron receptor
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base G (3/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGGCCGGTGACTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGACGCGCCCTCCAGGCTGCCGT  >  NC_002947/425211‑425505
                                                                                                                                                    |                                                                                                                                                  
gggCAGGCCGGTGACTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCa                                                                                                                                                    <  7:67684/149‑1 (MQ=255)
      gCCGGTGACTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGctcac                                                                                                                                              >  6:317187/1‑149 (MQ=255)
      gCCGGTGACTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGctcac                                                                                                                                              >  5:70125/1‑149 (MQ=255)
             aCTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAAc                                                                                                                                       >  3:60420/1‑149 (MQ=255)
              gtGCCAGCAACAGCAGGAACAGGGTACAGACGAGGCTGCTACAGGTGTGGATCAAGGTCCAGCGGCGGATGGTGGGGCTCTTCATCACTGTACCGATTTTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACt                                                                                                                                      <  6:325394/148‑1 (MQ=255)
              cTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACt                                                                                                                                      >  5:317757/1‑149 (MQ=255)
                gCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGAGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACtt                                                                                                                                     <  4:157074/148‑1 (MQ=255)
                 ccAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGt                                                                                                                                   <  6:30544/149‑1 (MQ=255)
                   aGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGa                                                                                                                                         >  3:261319/1‑141 (MQ=255)
                           cagGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCt                                                                                                                         >  3:133225/1‑149 (MQ=255)
                                aCAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTAGGTTGGAACCCGGGGCCGCAAGGCGCCCCCCTCTCACGCAGAACATGTAAGTTGCGCTGGCGa                                                                                                                    >  8:152967/1‑149 (MQ=255)
                                 cAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCCAGGCGGCCCCCGCTAACACAGAACATGTAACTGGCGCAGGCgg                                                                                                                    >  8:114053/1‑147 (MQ=255)
                                   gggTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACaa                                                                                                                 <  4:133225/149‑1 (MQ=255)
                                    ggTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAAc                                                                                                                >  8:59934/1‑149 (MQ=255)
                                      tACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGc                                                                                                              >  3:326995/1‑149 (MQ=255)
                                             cAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCGGCTCACTGAGAAGGTGTAAGTGGCGCTGGCGACAGCGCTgcgcgc                                                                                                       >  8:219153/1‑147 (MQ=255)
                                                    ctCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGcc                                                                                                 <  6:317757/148‑1 (MQ=255)
                                                     tCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCg                                                                                                >  1:257462/1‑148 (MQ=255)
                                                     tCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCACCACACTCAGAACATGTAAGTGGCGCTGGCGACAACGCTGCGCTCAACGCCg                                                                                                >  6:7414/1‑148 (MQ=255)
                                                               gATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCAGCAAGGCGGCCCCAGCTCACTCAGAACTTTTAAGTGGCGCTTGCGCCAACTCTGCGCTCATCGCCGTAGTAGCac                                                                                       >  5:163777/1‑146 (MQ=255)
                                                                  cAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGcc                                                                                                 <  8:136940/134‑1 (MQ=255)
                                                                  cAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGcc                                                                                                 >  7:136940/1‑134 (MQ=255)
                                                                     ggACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGa                                                                               <  4:60420/149‑1 (MQ=255)
                                                                     ggACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGa                                                                               <  5:169113/149‑1 (MQ=255)
                                                                            cggcggATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACaa                                                                        <  6:196277/149‑1 (MQ=255)
                                                                             ggcggATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAg                                                                       <  7:219153/149‑1 (MQ=255)
                                                                               cggATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTa                                                                     <  6:70125/149‑1 (MQ=255)
                                                                               cggATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTa                                                                     <  7:152967/149‑1 (MQ=255)
                                                                                  aTGGTGGGACTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTa                                                                     >  3:298675/1‑146 (MQ=255)
                                                                                   tggtggGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTagaga                                                                 <  4:272410/149‑1 (MQ=255)
                                                                                    ggtggGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTagaga                                                                 <  7:114053/148‑1 (MQ=255)
                                                                                        gggCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTaa                                                            <  4:326995/149‑1 (MQ=255)
                                                                                            tctTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCt                                                        >  5:149771/1‑149 (MQ=255)
                                                                                             ctTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCtt                                                       >  5:161895/1‑149 (MQ=255)
                                                                                              ttCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCCGCTCACTCAGAACGTGTTAGTGGCGCTGGCGACAACGCGGCGCTCATCGCCGGAGTAGCAGTATTAGGAg                                                                              >  4:225822/1‑125 (MQ=255)
                                                                                              ttCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAg                                                                              <  3:225822/125‑1 (MQ=255)
                                                                                                 atcaCGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTc                                                   <  7:59934/149‑1 (MQ=255)
                                                                                                    aCGGTACGTATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCTGTAGTAGGAGTCACAAGAAGAGAGGTAATCCTTGTTcagc                                                <  6:163777/149‑1 (MQ=255)
                                                                                                    aCGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTcag                                                 <  5:317187/148‑1 (MQ=255)
                                                                                                     cGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTcagca                                               <  4:298675/149‑1 (MQ=255)
                                                                                                      ggTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTcagcag                                              <  6:149771/149‑1 (MQ=255)
                                                                                                      ggTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTcagcag                                              >  4:63021/1‑149 (MQ=255)
                                                                                                          ccGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTtg                                          <  5:7414/149‑1 (MQ=255)
                                                                                                            gATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATc                                                          <  8:236921/131‑1 (MQ=255)
                                                                                                            gATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATc                                                          >  7:236921/1‑131 (MQ=255)
                                                                                                             aTTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAg                                                                       >  8:260828/1‑117 (MQ=255)
                                                                                                             aTTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAg                                                                       <  7:260828/117‑1 (MQ=255)
                                                                                                                   gtCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTg                                 >  5:238839/1‑149 (MQ=255)
                                                                                                                    tCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGa                                <  2:257462/149‑1 (MQ=255)
                                                                                                                    tCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGa                                <  3:94285/149‑1 (MQ=255)
                                                                                                                    tCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGAAGTAGCAGTAGTAGGAGTTACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGa                                >  2:52889/1‑149 (MQ=255)
                                                                                                                           tgagctggggCCGCCTTGCGGCCCCGGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGcgac                         >  7:212059/7‑149 (MQ=255)
                                                                                                                             agctggggCCGCCTTGCGGCCCCGGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATc                                                          <  8:172618/110‑1 (MQ=255)
                                                                                                                             agctggggCCGCCTTGCGGCCCCGGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATc                                                          >  7:172618/5‑114 (MQ=255)
                                                                                                                              cccGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGcgaccga                      >  2:226548/1‑149 (MQ=255)
                                                                                                                               ccGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGcgaccgac                     <  3:63021/149‑1 (MQ=255)
                                                                                                                                 ggggCCGCCTTGCGGCCCCGGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGAcgc                   <  8:212059/149‑1 (MQ=255)
                                                                                                                                       gCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGACGCGCCCTc             >  3:239452/1‑149 (MQ=255)
                                                                                                                                          aGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTTACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGACGCGCCCTCCAg          <  1:52889/149‑1 (MQ=255)
                                                                                                                                               gcCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGACGCGCCCTCCAGGCTGc     >  2:226570/1‑149 (MQ=255)
                                                                                                                                                 cccAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGt                                  >  7:233376/1‑118 (MQ=255)
                                                                                                                                                  ccAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGAAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGAGACCGACGCGCCCTCCAGGCTGCCGt  >  5:14371/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGGCAGGCCGGTGACTGCCAGCAACAGCAGGAACAGGGTACAGACCAGGCTGCTCCAGGTGTGGATCAAGGACCAGCGGCGGATGGTGGGGCTCTTCATCACGGTACCGATTGTTGTCGGTTGGAACCCGGGGCCGCAAGGCGGCCCCAGCTCACTCAGAACTTGTAAGTGGCGCTGGCGACAACGCTGCGCTCATCGCCGTAGTAGCAGTAGTAGGAGTCACAAGTAGAGAGGTAATCCTTGTTCAGCAGGTTGGTGGCGGTGACCGCGACCGACGCGCCCTCCAGGCTGCCGT  >  NC_002947/425211‑425505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑