Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 464,149 G→A 10.6% D316D (GAC→GAT)  davB ← lysine 2‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947464,1490GA10.6% 124.0 / 4.5 47D316D (GAC→GAT) davBlysine 2‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base A (3/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGACTGCATGTAGCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCAC  >  NC_002947/464006‑464295
                                                                                                                                               |                                                                                                                                                  
gacgacTGCATGTAGCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGt                                                                                                                                               <  6:123411/149‑1 (MQ=255)
 acgacTGCATGTAGCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGt                                                                                                                                               >  7:161206/1‑148 (MQ=255)
    acTGCATGTAGCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCACCCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGAcc                                                                                                                                           >  2:185049/1‑149 (MQ=255)
              gCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGTTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAggcggc                                                                                                                                 <  1:242269/149‑1 (MQ=255)
                      ggTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATcggcg                                                                                                                         <  1:185049/149‑1 (MQ=255)
                           aGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGcgcg                                                                                                                    <  2:15508/149‑1 (MQ=255)
                                cATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGAGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCaa                                                                                                               >  4:214497/1‑149 (MQ=255)
                                    cacaTCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATgcg                                                                                                           >  5:347893/1‑149 (MQ=255)
                                       aTCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCtt                                                                                                        <  3:40191/149‑1 (MQ=255)
                                        tCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGAGGCGCGGGCAATGCGCtt                                                                                                        >  5:93683/1‑148 (MQ=255)
                                          ttttGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAggcgg                                                                                                                                  <  2:337622/120‑1 (MQ=255)
                                          ttttGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAggcgg                                                                                                                                  >  1:337622/1‑120 (MQ=255)
                                          ttttGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGAc                                                                                                     >  7:205030/1‑149 (MQ=255)
                                            ttGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGAcc                                                                                                    <  8:161206/148‑1 (MQ=255)
                                                      aGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCgg                                                                                         >  3:192771/1‑149 (MQ=255)
                                                               tCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGcgccgc                                                                                 >  6:50659/1‑148 (MQ=255)
                                                                  cAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATg                                                                             >  7:217498/1‑149 (MQ=255)
                                                                     tCGATCTGGGTGGTCAGCAACCAGGTCTGGAAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCTCTTGCCCCCGGTACGCGGTGCGCCGCCATGCAg                                                                          >  8:285642/1‑149 (MQ=255)
                                                                       gATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCg                                                                        >  2:28812/1‑149 (MQ=255)
                                                                             ggtggtCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCt                                                                    >  4:27679/1‑147 (MQ=255)
                                                                             ggtggtCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTc                                                                   <  7:313280/148‑1 (MQ=255)
                                                                                 gtCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTATCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCt                                                              >  4:232434/1‑149 (MQ=255)
                                                                                  tCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTg                                                             >  7:248273/1‑149 (MQ=255)
                                                                                         ccAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCct                                                      >  3:306072/1‑149 (MQ=255)
                                                                                            ggTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTg                                                   <  5:50659/149‑1 (MQ=255)
                                                                                            ggTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTg                                                   <  4:192771/149‑1 (MQ=255)
                                                                                              tCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGc                                                 >  3:72668/1‑149 (MQ=255)
                                                                                                tGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGcc                                                >  5:327673/1‑148 (MQ=255)
                                                                                                 ggCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTg                                                   >  8:62158/1‑144 (MQ=255)
                                                                                                 ggCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTg                                                   <  7:62158/144‑1 (MQ=255)
                                                                                                  gCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAg                                                                 <  6:89103/129‑1 (MQ=255)
                                                                                                  gCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAg                                                                 >  5:89103/1‑129 (MQ=255)
                                                                                                  gCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCaa                                              <  8:217498/148‑1 (MQ=255)
                                                                                                         gCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCa                                      <  6:327673/149‑1 (MQ=255)
                                                                                                         gCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTATCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACg                                        >  8:255468/1‑147 (MQ=255)
                                                                                                         gCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTATCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTAGCCAATGCACg                                        >  8:250997/1‑147 (MQ=255)
                                                                                                           gAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGc                                    >  8:241981/1‑149 (MQ=255)
                                                                                                           gAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGc                                    <  8:205030/149‑1 (MQ=255)
                                                                                                                   cgcTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCg                              <  7:133071/147‑1 (MQ=255)
                                                                                                                    gcTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCTGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTa                           <  6:93683/149‑1 (MQ=255)
                                                                                                                      tGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCAGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAAt                                             >  8:78761/1‑129 (MQ=255)
                                                                                                                      tGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCAGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAAt                                             <  7:78761/129‑1 (MQ=255)
                                                                                                                                  tATCGCCCCAGTTATCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATg             <  7:255468/149‑1 (MQ=255)
                                                                                                                                  tATCGCCCCAGTTATCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATg             <  7:250997/149‑1 (MQ=255)
                                                                                                                                      gCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCtt         <  3:27679/149‑1 (MQ=255)
                                                                                                                                       ccccAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTtg        >  5:186730/1‑149 (MQ=255)
                                                                                                                                             ttGTCGGTGCCCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCAc  <  3:214497/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GACGACTGCATGTAGCGGGTACGGTCCAGGGCCATCCACATCTTTTGCGAGAACAGCGATTCCTCGCAGTCGATCTGGGTGGTCAGCAACCAGGTCTGGCAGGTGGCGAGTACTGCGCTGTAGTGGCGGGTATCGCCCCAGTTGTCGGTGACCGCCAGGCGGCCATCGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCAC  >  NC_002947/464006‑464295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑