Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 502,690 T→A 6.9% F327I (TTC→ATC)  PP_0413 → polyamine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947502,6900TA 6.9% 173.7 / 2.6 58F327I (TTC→ATC) PP_0413polyamine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base A (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TGGTGTTCAACCGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACTTCGTCGCCTTCTAC  >  NC_002947/502544‑502836
                                                                                                                                                  |                                                                                                                                                  
tGGTGTTCAACCGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGAGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTAGCTGGGCTGCCACCCGTTCGCCAGTTTc                                                                                                                                                  >  7:308899/1‑149 (MQ=255)
       cAACCGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCgg                                                                                                                                            <  4:300027/148‑1 (MQ=255)
         aCCGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGgtg                                                                                                                                         >  7:48264/1‑149 (MQ=255)
          ccGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGgtg                                                                                                                                         >  7:43292/1‑148 (MQ=255)
          ccGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGgtg                                                                                                                                         >  4:187828/1‑148 (MQ=255)
                aGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTACCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTcc                                                                                                                                  >  1:249398/1‑149 (MQ=255)
                   cGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGc                                                                                                                               <  8:53900/149‑1 (MQ=255)
                    gTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGAAACTGGCgaacg                                                                                                                                         <  6:228405/138‑5 (MQ=255)
                    gTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGAAACTGGCgaacg                                                                                                                                         >  5:228405/1‑134 (MQ=255)
                         cATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGa                                                                                                                            <  3:187828/146‑1 (MQ=255)
                         cATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGa                                                                                                                            <  8:43292/146‑1 (MQ=255)
                           tctcGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTAc                                                                                                                       >  6:171659/1‑149 (MQ=255)
                            ctcGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGAAACTGGCgaac                                                                                                                                          <  8:103597/129‑4 (MQ=255)
                            ctcGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGAAACTGGCgaac                                                                                                                                          >  7:103597/1‑126 (MQ=255)
                                      cacaTCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTgg                                                                                                             <  3:322799/148‑1 (MQ=255)
                                      cacaTCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTgg                                                                                                             <  8:132168/148‑1 (MQ=255)
                                      cacaTCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTgg                                                                                                             >  4:322799/1‑148 (MQ=255)
                                       acaTCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGgc                                                                                                           <  8:128157/149‑1 (MQ=255)
                                            ctgctgCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCg                                                                                                       <  8:48264/148‑1 (MQ=255)
                                                    gTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCctgc                                                                                              <  8:308899/149‑1 (MQ=255)
                                                        aTGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGgt                                                                                          >  5:212427/1‑149 (MQ=255)
                                                        aTGATCCTGCCGCTGTACAGCGTGATGAAGGGTATCTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGgt                                                                                          >  3:91335/1‑149 (MQ=255)
                                                         tGATCCTGCCGCTGTACAGCGTGATGAAGGGTAATTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTCCATGCTGgtg                                                                                         >  8:133308/1‑149 (MQ=255)
                                                           aTCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGgtgt                                                                                        >  4:97124/1‑148 (MQ=255)
                                                             ccTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCa                                                                                     >  8:54892/1‑149 (MQ=255)
                                                              cTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCct                                                                                                >  7:31116/1‑137 (MQ=255)
                                                              cTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCct                                                                                                <  8:31116/137‑1 (MQ=255)
                                                                 ccGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATcc                                                                                  <  5:171659/148‑1 (MQ=255)
                                                                 ccGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATcc                                                                                  <  3:97124/148‑1 (MQ=255)
                                                                   gCTGTACAGCGTGATTCAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTg                                                                               <  4:44351/149‑1 (MQ=255)
                                                                   gCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTg                                                                               <  8:9236/149‑1 (MQ=255)
                                                                   gCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTg                                                                               <  4:41561/149‑1 (MQ=255)
                                                                           gCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGAc                                                                                                                           >  1:152103/1‑97 (MQ=255)
                                                                           gCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGAc                                                                                                                           <  2:152103/97‑1 (MQ=255)
                                                                            cGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGt                                                                       >  6:293374/1‑148 (MQ=255)
                                                                             gTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTta                                                                     <  7:54892/149‑1 (MQ=255)
                                                                                          tttCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCg                                                        >  4:150681/1‑149 (MQ=255)
                                                                                              cccGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCtt                                                                                >  7:89828/1‑121 (MQ=255)
                                                                                              cccGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCtt                                                                                <  8:89828/121‑1 (MQ=255)
                                                                                               ccGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCctgct                                                                                             >  7:227819/1‑107 (MQ=255)
                                                                                               ccGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCctgct                                                                                             <  8:227819/107‑1 (MQ=255)
                                                                                                   gCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGctgctg                                               >  6:327597/1‑149 (MQ=255)
                                                                                                   gCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGctgctg                                               <  2:249398/149‑1 (MQ=255)
                                                                                                       cATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTCTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTa                                           >  8:169859/1‑149 (MQ=255)
                                                                                                        aTGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAg                                          >  5:105619/1‑149 (MQ=255)
                                                                                                           cgtgcgGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTcc                                       <  2:97851/149‑1 (MQ=255)
                                                                                                           cgtgcgGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTcc                                       <  3:150681/149‑1 (MQ=255)
                                                                                                           cgtgcgGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTc                                        <  7:169859/148‑1 (MQ=255)
                                                                                                                ggcgATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAAc                                   >  6:165501/1‑148 (MQ=255)
                                                                                                                                  ccACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGCTGTGGGCGCCGGTTGCCTGCTGGTGTTCATACTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACt                >  8:99979/1‑149 (MQ=255)
                                                                                                                                    aCCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACTtc              <  6:105619/149‑1 (MQ=255)
                                                                                                                                     cccGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCg                                                                         <  6:101233/89‑1 (MQ=255)
                                                                                                                                      ccGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATg                          >  6:131653/1‑135 (MQ=255)
                                                                                                                                      ccGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATg                          <  5:131653/135‑1 (MQ=255)
                                                                                                                                            gCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACACCCAGATGGTCAGCTACTTCGTCGccgt      >  7:144874/1‑147 (MQ=255)
                                                                                                                                              cAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGACAGCTACTTCGTCGCCTTc     >  3:23273/1‑148 (MQ=255)
                                                                                                                                               aGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACTTCGTCGCCTTCTa   <  6:199595/149‑1 (MQ=255)
                                                                                                                                                gTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACTTCGTCGCCTTCTac  <  5:37627/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGTGTTCAACCGCACAGGCGTCTACATCTCGATGGTGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGGGTATTTCCCCGAGCTACATGCGTGCGGCGATCTCGCTGGGCTGCCACCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACTTACGCCGGTGTGGGCGCCGGTTGCCTGCTGGTGTTCATCCTTGCCATCGGTTACTACATCACCCCGGCGCTGCTGGGTAGTCCGAACGACCAGATGGTCAGCTACTTCGTCGCCTTCTAC  >  NC_002947/502544‑502836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑