Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 556,989 G→T 9.8% R13L (CGT→CTT)  rpsN → 30S ribosomal protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947556,9890GT 9.8% 188.5 / 8.0 61R13L (CGT→CTT) rpsN30S ribosomal protein S14
Reads supporting (aligned to +/- strand):  ref base G (31/24);  new base T (3/3);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAAC  >  NC_002947/556842‑557137
                                                                                                                                                   |                                                                                                                                                    
tctGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGt                                                                                                                                                     >  4:78783/1‑149 (MQ=255)
 cggcgcGGTGAGGACATCACCCAGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCGGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTc                                                                                                                                                    <  8:165082/147‑1 (MQ=255)
    cgcgGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGTGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCa                                                                                                                                                   <  6:629/147‑1 (MQ=255)
     gcgGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCt                                                                                                                                                >  2:162305/1‑149 (MQ=255)
         tttGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTAAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTcgcg                                                                                                                                            <  8:71351/149‑1 (MQ=255)
             gACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGttt                                                                                                                                        >  4:22172/1‑147 (MQ=255)
             gACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTa                                                                                                                                         <  3:78783/148‑1 (MQ=255)
                aTCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTcgcg                                                                                                                                            <  5:223592/142‑1 (MQ=255)
                aTCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTcgcg                                                                                                                                            >  6:223592/1‑142 (MQ=255)
                aTCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCa                                                                                                                                      >  4:57682/1‑148 (MQ=255)
                aTCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCa                                                                                                                                      >  6:322736/1‑148 (MQ=255)
                 tCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttttca                                                                                                                                    <  3:22172/149‑7 (MQ=255)
                 tCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCa                                                                                                                                      >  1:86686/1‑147 (MQ=255)
                   aCCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAg                                                                                                                                   <  1:165130/148‑1 (MQ=255)
                    cccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGtt                                                                                                                                 >  6:132784/1‑149 (MQ=255)
                     ccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTc                                                                                                                                >  6:266377/1‑149 (MQ=255)
                         accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGc                                                                                                                              <  5:129699/147‑1 (MQ=255)
                         accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                             <  6:268096/148‑1 (MQ=255)
                         accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                             <  3:57682/148‑1 (MQ=255)
                           caccacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                             >  1:53937/1‑146 (MQ=255)
                                     cGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgct                                                                                                                 >  5:69753/1‑148 (MQ=255)
                                             cgacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                        <  7:261632/149‑1 (MQ=255)
                                               acgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGc                                                                                                      >  2:60114/1‑149 (MQ=255)
                                                cgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTAACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGc                                                                                                      >  5:262038/1‑148 (MQ=255)
                                                 gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                    <  5:266377/149‑1 (MQ=255)
                                                    ggCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCa                                                                                                 >  6:222969/1‑149 (MQ=255)
                                                     gCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTc                                                                                                                                >  2:2563/1‑117 (MQ=255)
                                                     gCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTc                                                                                                                                <  1:2563/117‑1 (MQ=255)
                                                      ccGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATc                                                                                               <  1:60114/149‑1 (MQ=255)
                                                         cgcTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                        <  8:139625/137‑1 (MQ=255)
                                                         cgcTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                        >  7:139625/1‑137 (MQ=255)
                                                           ctctGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGCAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCaa                                                                                          <  5:290515/149‑1 (MQ=255)
                                                                ctgcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAcc                                                                                        <  5:222969/146‑1 (MQ=255)
                                                                ctgcGTGCTTTCAAGTTCCCGTTCCGCAACTGAATGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAcc                                                                                        <  5:322736/146‑1 (MQ=255)
                                                                   cGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGG‑AGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                                 >  1:170951/1‑149 (MQ=255)
                                                                    gTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                                 <  5:132784/149‑1 (MQ=255)
                                                                           cAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc                                                                            >  8:225346/1‑147 (MQ=255)
                                                                                   cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                                  >  3:110795/1‑149 (MQ=255)
                                                                                     ttCCGGAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGtt                                                                <  6:93508/149‑1 (MQ=255)
                                                                                        cGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTc                                                              <  6:325487/148‑1 (MQ=255)
                                                                                           aaCTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTg                                                          >  5:347380/1‑149 (MQ=255)
                                                                                               gATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                         >  8:132883/1‑146 (MQ=255)
                                                                                               gATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                         <  7:132883/146‑1 (MQ=255)
                                                                                                 ttGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg                                                    >  7:122350/1‑149 (MQ=255)
                                                                                                    gAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCg                                                 <  4:110795/149‑1 (MQ=255)
                                                                                                          gAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                            >  7:170702/1‑148 (MQ=255)
                                                                                                          gAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGc                                           <  6:69753/149‑1 (MQ=255)
                                                                                                             aaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAg                                         >  2:330748/1‑148 (MQ=255)
                                                                                                                 gCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagca                                    <  1:162305/149‑1 (MQ=255)
                                                                                                                  ccAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcag                                   >  8:212391/1‑149 (MQ=255)
                                                                                                                   cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcagc                                  >  5:321990/1‑149 (MQ=255)
                                                                                                                      gaagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAc                               >  5:22239/1‑149 (MQ=255)
                                                                                                                              tGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGc                        >  8:316608/1‑148 (MQ=255)
                                                                                                                                 aaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCg                                                 <  2:101183/120‑1 (MQ=255)
                                                                                                                                 aaaaCCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCg                                                 >  1:101183/1‑120 (MQ=255)
                                                                                                                                      cgtgAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTc               >  8:60703/4‑149 (MQ=255)
                                                                                                                                              gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc       >  8:97816/1‑149 (MQ=255)
                                                                                                                                              gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc       >  6:133172/1‑149 (MQ=255)
                                                                                                                                               aaGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgcg      <  2:86686/149‑1 (MQ=255)
                                                                                                                                               aaGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGACTCGCGCCTgcg      >  7:109744/1‑149 (MQ=255)
                                                                                                                                                aGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCAGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgcg      >  1:113912/1‑148 (MQ=255)
                                                                                                                                                   gTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAAc  >  4:281110/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAAC  >  NC_002947/556842‑557137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑