Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 613,177 C→T 8.1% W335* (TGG→TGA)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947613,1770CT 8.1% 139.1 / 2.4 49W335* (TGG→TGA) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base T (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CCGCCAAGGTCACGGCATGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCCTTCTTGGTCAC  >  NC_002947/613029‑613320
                                                                                                                                                     |                                                                                                                                               
ccgccAAGGTCACGGCATGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGc                                                                                                                                                 <  8:203410/149‑1 (MQ=255)
         tCACGGCATGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGcc                                                                                                                                         >  4:164106/1‑148 (MQ=255)
           aCGGCATGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGa                                                                                                                                       <  4:23765/148‑1 (MQ=255)
                aTGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACcg                                                                                                                                 >  2:308153/1‑149 (MQ=255)
                           cGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCACCCCTGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTAttt                                                                                                                       <  1:97978/149‑1 (MQ=255)
                           cGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTAtt                                                                                                                        <  3:240933/147‑1 (MQ=255)
                             aTCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCgg                                                                                                                    <  5:30498/149‑1 (MQ=255)
                             aTCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCgg                                                                                                                    >  3:227992/1‑149 (MQ=255)
                                  aaCGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGc                                                                                                               <  3:7322/149‑1 (MQ=255)
                                          aGTAGCGCTGAGGGTAGAGCTCGCTGAAGTCGACCAGGTCCGAGCAGTGGTTCATTGCCGGGCTAATGGCGA‑CAAGGCGGTTGTCGGCGGCGGCCATGTCGCTCAGCGACTGGGCGAGCACGAGGGAGTATTTCGGGCAGCTGGCTttc                                                                                                       >  4:233256/1‑149 (MQ=255)
                                                 cTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTTCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTc                                                                                                                      <  6:185855/127‑1 (MQ=255)
                                                 cTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTTCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTc                                                                                                                      >  5:185855/1‑127 (MQ=255)
                                                             ctcgctGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGt                                                                                    >  3:103335/1‑149 (MQ=255)
                                                              tcgctGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGTCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTg                                                                                     <  5:70040/147‑1 (MQ=255)
                                                              tcgctGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGTCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTg                                                                                     >  6:70040/1‑147 (MQ=255)
                                                              tcgctGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTc                                                                                   >  3:55940/1‑149 (MQ=255)
                                                                gctGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTcgg                                                                                 <  5:266833/149‑1 (MQ=255)
                                                                  tGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGc                                                                                        <  5:177198/140‑1 (MQ=255)
                                                                  tGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGc                                                                                        >  6:177198/1‑140 (MQ=255)
                                                                    aaGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGTCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTcggccgg                                                                             >  4:233532/1‑149 (MQ=255)
                                                                    aaGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTcggccgg                                                                             <  3:67873/149‑1 (MQ=255)
                                                                       tCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTc                                                                          <  1:308153/149‑1 (MQ=255)
                                                                            cAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCTCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCt                                                                     <  4:55940/149‑1 (MQ=255)
                                                                               gTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGTCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTgg                                                                  <  3:233532/149‑1 (MQ=255)
                                                                               gTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTgg                                                                  <  3:164106/149‑1 (MQ=255)
                                                                                        ttccttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGt                                                                 <  7:80421/141‑1 (MQ=255)
                                                                                        ttccttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGt                                                                 >  8:80421/1‑141 (MQ=255)
                                                                                          ccttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTg                                                        <  6:198340/148‑1 (MQ=255)
                                                                                           cttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGc                                                                                        >  5:52674/1‑115 (MQ=255)
                                                                                           cttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGc                                                                                        <  6:52674/115‑1 (MQ=255)
                                                                                           cttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTgg                                                       >  1:244583/1‑148 (MQ=255)
                                                                                            ttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGAt                                                              <  6:35066/140‑1 (MQ=255)
                                                                                            ttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGAt                                                              >  5:35066/1‑140 (MQ=255)
                                                                                            ttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTgg                                                       >  8:295890/1‑147 (MQ=255)
                                                                                            ttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTgg                                                       >  7:159861/1‑147 (MQ=255)
                                                                                            ttcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTgg                                                       >  8:106881/1‑147 (MQ=255)
                                                                                             tcATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAg                                                    >  1:304255/1‑149 (MQ=255)
                                                                                               aTTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGc                                                   <  7:103575/148‑1 (MQ=255)
                                                                                                               cca‑ccaGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCg                                  <  4:103335/149‑1 (MQ=255)
                                                                                                                ca‑ccaGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCgg                                 >  4:274385/1‑149 (MQ=255)
                                                                                                                ca‑ccaGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCgg                                 >  8:17118/1‑149 (MQ=255)
                                                                                                                          gTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGa                        <  7:17118/149‑1 (MQ=255)
                                                                                                                                      ggcCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCg                                  <  5:48570/127‑1 (MQ=255)
                                                                                                                                      ggcCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCg                                  >  6:48570/1‑127 (MQ=255)
                                                                                                                                        ccATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCcttc          >  6:81178/1‑149 (MQ=255)
                                                                                                                                          aTGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCcttctt        <  2:304255/149‑1 (MQ=255)
                                                                                                                                           tGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCCTTCTTg       <  7:106881/149‑1 (MQ=255)
                                                                                                                                              cGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCcttctt        <  8:101615/145‑1 (MQ=255)
                                                                                                                                              cGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCcttctt        >  7:101615/1‑145 (MQ=255)
                                                                                                                                                 acaGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCCTTCTTGGTcac  >  5:180714/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CCGCCAAGGTCACGGCATGCTGCTCTGCGATCGCAACGTCAAAGTAGCGCTCAGGGTAGCGCTCGCTGAAGTCGACCAGGTCCGAGCCTTCCTTCATTGCCGGGGTAATGCCCA‑CCAGGCGGTTGTCGGCGGCGGCCATGTCGCACAGCCACTGGCCGAACACCGCGGAGTATTTCGGGCCGCTGGCTTTCTTCGGCGCGGCGGGCTTGTCGGCCGGCTCCAGCTTGGTGATGGCGTGGTAGCCGATCGGGTCAATCTCGGCAGGGGCGAAGCCTTTGCCCTTCTTGGTCAC  >  NC_002947/613029‑613320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑