Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 613,444 T→C 7.8% P246P (CCA→CCG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947613,4440TC 7.8% 142.9 / 2.5 51P246P (CCA→CCG) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

AGCCTTTGCCCTTCTTGGTCACCACATGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGTTGGAC  >  NC_002947/613299‑613585
                                                                                                                                                 |                                                                                                                                             
aGCCTTTGCCCTTCTTGGTCACCACATGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGc                                                                                                                                            <  5:153869/149‑1 (MQ=255)
   cTTTGCCCTTCTTGGTCACCACATGCAGGAACTGCGGACCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCAc                                                                                                                                          >  3:284023/1‑148 (MQ=255)
      tGCCCTTCTTGGTCACCACATGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCa                                                                                                                                        <  8:301588/147‑1 (MQ=255)
              ttGGTCACCACATGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCtt                                                                                                                              >  7:220940/1‑149 (MQ=255)
                      cacaTGCAGGAACTGCGGGACTTTCATGTCAAGCATGTTTCGCAGGGTGGCGATCAAGGTGTGCAGCTCTTGTCCGTCGATCCGGCCGATGTAGTTAAATCCAAGCACTTAGAACACCCTGCCTGGCACCAGAATGCCCATGGTCTAtt                                                                                                                      >  6:177501/1‑149 (MQ=255)
                          tGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTc                                                                                                                  <  4:12558/149‑1 (MQ=255)
                            cAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGGGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCgg                                                                                                                <  5:329637/149‑1 (MQ=255)
                             aGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGt                                                                                                               >  6:306043/1‑149 (MQ=255)
                             aGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGt                                                                                                               <  8:220940/149‑1 (MQ=255)
                             aGGAACTGCGGACCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGt                                                                                                               <  4:284023/149‑1 (MQ=255)
                                     cGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGATGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTg                                                                                                              >  1:216764/1‑142 (MQ=255)
                                     cGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGATGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTg                                                                                                              <  2:216764/142‑1 (MQ=255)
                                        gCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCaa                                                                                                    >  5:180323/1‑149 (MQ=255)
                                           tttCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAAttt                                                                                                 >  2:36540/1‑149 (MQ=255)
                                               aGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCa                                                                                             <  5:306043/149‑1 (MQ=255)
                                                 gTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAgg                                                                                           >  7:202965/1‑149 (MQ=255)
                                                    aCGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGgcgc                                                                                        >  3:255032/1‑149 (MQ=255)
                                                    aCGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGgcgc                                                                                        >  1:317016/1‑149 (MQ=255)
                                                      gCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCCGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCt                                                                                      >  6:136228/1‑149 (MQ=255)
                                                       cATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTg                                                                                     <  7:17830/149‑1 (MQ=255)
                                                       cATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCACTTGGCGTATTCATCGGTGAGAAGGGCAATTTCCCAGGCGCATg                                                                                     >  6:96891/1‑149 (MQ=255)
                                                                  gggTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCTCGAc                                                                          <  5:96891/149‑1 (MQ=255)
                                                                        cGATCATGGTGGGCAGGTCGTGGCCGTCGAGCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGTGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACt                                                                    <  5:177501/149‑1 (MQ=255)
                                                                             aTGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCtt                                                               <  2:221302/149‑1 (MQ=255)
                                                                              tggtggGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTACTtg                                                              >  6:252687/1‑149 (MQ=255)
                                                                                gtggGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTtgct                                                            <  2:317016/149‑1 (MQ=255)
                                                                                   ggCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCCGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTtgctgc                                                          <  5:136228/148‑1 (MQ=255)
                                                                                                cGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTc                                            <  5:114500/149‑1 (MQ=255)
                                                                                                    gATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGt                                        <  7:74316/149‑1 (MQ=255)
                                                                                                         ggCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTg                                   >  5:118180/1‑149 (MQ=255)
                                                                                                         ggCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCCGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTg                                   >  2:239055/1‑149 (MQ=255)
                                                                                                          gCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGc                                  <  1:240209/149‑1 (MQ=255)
                                                                                                          gCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCCGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGc                                  <  1:239055/149‑1 (MQ=255)
                                                                                                             gATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGc                               >  3:11161/1‑149 (MQ=255)
                                                                                                                  aGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTg                             >  8:216562/1‑146 (MQ=255)
                                                                                                                    ttCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGagag                        >  3:259991/1‑149 (MQ=255)
                                                                                                                      ccagcccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGa                      >  6:72859/1‑149 (MQ=255)
                                                                                                                        agcccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATc                    <  5:72859/149‑1 (MQ=255)
                                                                                                                          cccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCtt                  >  6:228074/1‑149 (MQ=255)
                                                                                                                           ccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTg                 <  4:255032/149‑1 (MQ=255)
                                                                                                                           ccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTg                 >  3:301457/1‑149 (MQ=255)
                                                                                                                           ccagcTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTg                 >  6:159733/1‑149 (MQ=255)
                                                                                                                               ctctTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCa             <  4:11161/149‑1 (MQ=255)
                                                                                                                                 ctTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTtg                                                              >  2:314549/1‑98 (MQ=255)
                                                                                                                                 ctTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTtg                                                              <  1:314549/98‑1 (MQ=255)
                                                                                                                                    cGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTa         <  4:259991/148‑1 (MQ=255)
                                                                                                                                     gAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGt       >  2:31825/1‑149 (MQ=255)
                                                                                                                                     gAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGt       >  7:81419/1‑149 (MQ=255)
                                                                                                                                       aCAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGTTg     <  8:81419/149‑1 (MQ=255)
                                                                                                                                          gCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGTTGGAc  <  6:180323/149‑1 (MQ=255)
                                                                                                                                           cGTGCCTGGCACCAGCATGCCCCTAGAGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGACTGGAGAGGATCTTGGCCAGGTAGTTGGa   >  7:211463/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AGCCTTTGCCCTTCTTGGTCACCACATGCAGGAACTGCGGGCCTTTCAGGTCACGCATGTTGCGCAGGGTGGCGATCATGGTGGGCAGGTCGTGGCCGTCGATCGGGCCGATGTAGTTCCAGCCCAGCTCTTCGAACAGCGTGCCTGGCACCAGCATGCCCTTGGCGTATTCCTCGGTGCGACGGGCAATTTCCCAGGCGCCTGGCAACCGCGACAGCACTTTCTTGCTGCCTTCGCGCATGCTCGCGTAGGTGCGGCTGGAGAGGATCTTGGCCAGGTAGTTGGAC  >  NC_002947/613299‑613585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑