Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 683,656 C→G 6.2% K65N (AAG→AAC)  PP_0585 ← MerR family metal‑sensitive transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947683,6560CG 6.2% 262.0 / 6.4 81K65N (AAG→AAC) PP_0585MerR family metal‑sensitive transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (36/40);  new base G (2/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

CCTGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAACGGATC  >  NC_002947/683511‑683803
                                                                                                                                                 |                                                                                                                                                   
ccTGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCGGCAAGTTg                                                                                                                                                  >  3:76496/1‑149 (MQ=255)
ccTGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTg                                                                                                                                                  >  1:216831/1‑149 (MQ=255)
 cTGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGc                                                                                                                                                 >  2:119673/1‑149 (MQ=255)
  tGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGcc                                                                                                                                                >  2:35960/1‑149 (MQ=255)
        tGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGAcctc                                                                                                                                          >  4:31554/1‑149 (MQ=255)
        tGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGAcctc                                                                                                                                          >  5:311244/1‑149 (MQ=255)
          cGAAACCAGCTCGCCTAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCACCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGAc                                                                                                                                             <  4:232325/144‑1 (MQ=255)
          cGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCACCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGAc                                                                                                                                             >  3:232325/1‑144 (MQ=255)
           gAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCGGCAAGTTGCTGAccttgtg                                                                                                                                       <  3:97854/149‑5 (MQ=255)
            aaaCCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCGTCAAGTTGCTGAccttgtgg                                                                                                                                      <  4:76496/149‑6 (MQ=255)
             aaCCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACctcctc                                                                                                                                       >  4:326673/1‑147 (MQ=255)
              aCCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAg                                                                                                                                    <  5:30007/149‑1 (MQ=255)
              aCCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAg                                                                                                                                    >  7:85158/1‑149 (MQ=255)
               ccAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAgg                                                                                                                                   <  1:119673/149‑1 (MQ=255)
                cAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAggg                                                                                                                                  <  5:330866/149‑1 (MQ=255)
                    tCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGaaaa                                                                                                                              <  4:291003/149‑1 (MQ=255)
                      gCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGaaaa                                                                                                                              <  2:2204/147‑1 (MQ=255)
                      gCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGaaaa                                                                                                                              <  2:278744/147‑1 (MQ=255)
                      gCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGaaaa                                                                                                                              <  6:185533/147‑1 (MQ=255)
                      gCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAg                                                                                                                             <  3:326673/148‑1 (MQ=255)
                      gCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGGCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGaaaa                                                                                                                              <  6:180865/147‑1 (MQ=255)
                       ccGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGcc                                                                                                                           >  5:197114/1‑149 (MQ=255)
                        cGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCACCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGccc                                                                                                                          <  4:62118/149‑1 (MQ=255)
                                  gcgcAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCgg                                                                                                                 >  2:40687/1‑148 (MQ=255)
                                          tCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCtt                                                                                                         >  5:50382/1‑148 (MQ=255)
                                            aCCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTtgat                                                                                                      <  2:232707/149‑1 (MQ=255)
                                            aCCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTtg                                                                                                        <  3:31554/147‑1 (MQ=255)
                                              cAGCTCCTCGATGCACCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAg                                                                                                                                    <  7:203514/117‑1 (MQ=255)
                                              cAGCTCCTCGATGCACCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAg                                                                                                                                    >  8:203514/1‑117 (MQ=255)
                                                gCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAg                                                                                                  <  8:85158/149‑1 (MQ=255)
                                                  tcctcGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGc                                                                                                >  3:275386/1‑149 (MQ=255)
                                                    ctcGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTtgat                                                                                                      <  4:173844/141‑1 (MQ=255)
                                                    ctcGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTtgat                                                                                                      >  3:173844/1‑141 (MQ=255)
                                                    ctcGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGc                                                                                                >  6:188179/1‑147 (MQ=255)
                                                       gATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGt                                                                                           >  3:162768/1‑149 (MQ=255)
                                                        aTGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGtt                                                                                          <  4:284076/149‑1 (MQ=255)
                                                                 ttcagttcaTCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTc                                                                                 <  2:216831/149‑1 (MQ=255)
                                                                     gttcaTCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGTACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTgc                                                                             >  2:79204/1‑149 (MQ=255)
                                                                        caTCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGcc                                                                          >  7:247856/1‑149 (MQ=255)
                                                                        caTCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGcc                                                                          >  2:333253/1‑149 (MQ=255)
                                                                         aTCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCt                                                                         >  4:282485/1‑149 (MQ=255)
                                                                          tCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGTTGAAGGCCAGGTTGTGCAAGTCTTGCGCCTg                                                                        <  1:35960/149‑1 (MQ=255)
                                                                          tCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTg                                                                        <  1:40687/149‑1 (MQ=255)
                                                                           cGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAAGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTgg                                                                       >  5:218145/1‑149 (MQ=255)
                                                                             aTGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTa                                                                     <  5:303238/149‑1 (MQ=255)
                                                                              tgtgGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTa                                                                     >  6:182401/1‑148 (MQ=255)
                                                                                  gtgCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAg                                                                                                  >  8:174340/1‑115 (MQ=255)
                                                                                  gtgCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAg                                                                                                  <  7:174340/115‑1 (MQ=255)
                                                                                  gtgCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGc                                                                <  5:311713/149‑1 (MQ=255)
                                                                                   ggCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCg                                                               <  4:32659/148‑1 (MQ=255)
                                                                                     cATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCgg                                                              <  5:188179/148‑1 (MQ=255)
                                                                                                 cTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGc                                                 >  6:52988/1‑149 (MQ=255)
                                                                                                  ttCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCt                                                >  6:182997/1‑149 (MQ=255)
                                                                                                     acaTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAAGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGc                                              <  6:218145/148‑1 (MQ=255)
                                                                                                      caTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGcc                                                                                               <  5:225868/98‑1 (MQ=255)
                                                                                                      caTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGcc                                                                                               >  6:225868/1‑98 (MQ=255)
                                                                                                      caTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGa                                            <  4:275386/149‑1 (MQ=255)
                                                                                                       aTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTAc                                                                    >  5:167080/1‑124 (MQ=255)
                                                                                                       aTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTAc                                                                    <  6:167080/124‑1 (MQ=255)
                                                                                                       aTCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAg                                           >  4:177704/1‑149 (MQ=255)
                                                                                                                 gCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGttt                                 >  2:111304/1‑149 (MQ=255)
                                                                                                                   aCGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGTTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGa                               <  3:282485/149‑1 (MQ=255)
                                                                                                                    cGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAg                              <  8:247856/149‑1 (MQ=255)
                                                                                                                     gTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTg                                               >  8:80859/1‑131 (MQ=255)
                                                                                                                     gTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTg                                               <  7:80859/131‑1 (MQ=255)
                                                                                                                      ttGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAg                              >  7:208058/1‑147 (MQ=255)
                                                                                                                             tCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGAt                     <  6:311244/149‑1 (MQ=255)
                                                                                                                               cTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAg                              >  7:108199/1‑138 (MQ=255)
                                                                                                                               cTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAg                              <  8:108199/138‑1 (MQ=255)
                                                                                                                                 gCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGAt                 >  2:228688/1‑149 (MQ=255)
                                                                                                                                  ccACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGa                  <  6:50382/147‑1 (MQ=255)
                                                                                                                                  ccACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGAtt                >  5:84309/1‑149 (MQ=255)
                                                                                                                                  ccACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGAtt                <  8:208058/149‑1 (MQ=255)
                                                                                                                                    aggaGGTCGGCAAGTTGCTGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGa                               <  6:294671/129‑1 (MQ=255)
                                                                                                                                    aggaGGTCGGCAAGTTGCTGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGa                               >  5:294671/4‑132 (MQ=255)
                                                                                                                                      aaGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCgta            <  1:79204/149‑1 (MQ=255)
                                                                                                                                           cAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTCTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAAc       >  7:156151/1‑149 (MQ=255)
                                                                                                                                            aGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAAc       <  5:52988/148‑1 (MQ=255)
                                                                                                                                            aGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAACg      >  4:121549/1‑149 (MQ=255)
                                                                                                                                              cAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAACGGa    <  5:229152/149‑1 (MQ=255)
                                                                                                                                                aCTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCACTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAACGGatc  <  4:162768/149‑1 (MQ=255)
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CCTGACAGTGCGAAACCAGCTCGCCGAGGGTGTCGCGCAGGCTCACCAGCTCCTCGATGCGCCGGTTCAGTTCATCGATGTGGTGCATGGCCAGCGCCTTCACATCGGCGCTGGCACGTTGGCGGTCCTGCCACAAGGTCAGCAACTTGCCGACCTCCTCGAGGGAAAAGCCCAGGTCGCGGGAGCGCTTGATGAAGGCCAGGTTGTGCAAGTCTTGCGCCTGGTACAGGCGGTAGCCGCTGTCGCTGCGAGTGGCGGGTTTGAGCAGGCCGATGGATTCGTAGTAACGGATC  >  NC_002947/683511‑683803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑