Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	735,770	C→A	7.4%	A189A (GCG→GCT)	PP_0628 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	735,770	0	C	A	7.4%	167.5 / 4.1	54	A189A (GCG→GCT)	PP_0628	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base A (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

GTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATG  >  NC_002947/735622‑735917                                                                                                                                                     |                                                                                                                                                    gTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgc                                                                                                                                                     >  5:10967/1‑149 (MQ=255) gTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgc                                                                                                                                                     >  4:54588/1‑149 (MQ=255) gTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgc                                                                                                                                                     <  5:17018/149‑1 (MQ=255) gTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgc                                                                                                                                                     <  3:166132/149‑1 (MQ=255)  ttCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgcg                                                                                                                                                    <  1:288313/149‑1 (MQ=255)  ttCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgcg                                                                                                                                                    >  5:174687/1‑149 (MQ=255)             gcTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTgg                                                                                                                                         >  1:195807/1‑149 (MQ=255)                tGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGc                                                                                                                                       >  5:200112/1‑148 (MQ=255)                  aaaCAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCaaa                                                                                                                                    <  3:54588/149‑1 (MQ=255)                      aaaTGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACa                                                                                                                                  <  4:76701/147‑1 (MQ=255)                      aaaTGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACa                                                                                                                                  >  3:76701/1‑147 (MQ=255)                            tAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGt                                                                                                                                >  7:149433/1‑143 (MQ=255)                            tAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGt                                                                                                                                <  8:149433/143‑1 (MQ=255)                            tAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCgg                                                                                                                          <  6:200112/149‑1 (MQ=255)                                   ttCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCATGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTTGGCAAACAGTACCCGGGCAAAg                                                                                                                    <  4:260468/148‑1 (MQ=255)                                        tCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGACGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCcaaccttt                                                                                                                <  4:101903/147‑6 (MQ=255)                                        tCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAACCCGTACCCGGGCAAAGGTTGGc                                                                                                              >  7:84305/1‑149 (MQ=255)                                        tCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGc                                                                                                              >  4:90951/1‑149 (MQ=255)                                          ccTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAg                                                                                                                    >  4:68250/1‑141 (MQ=255)                                          ccTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAg                                                                                                                    <  3:68250/141‑1 (MQ=255)                                          ccTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGccc                                                                                                            <  7:60432/149‑1 (MQ=255)                                          ccTGGCGCCGAGTGTACGCAATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCGTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGccc                                                                                                            <  7:187764/149‑1 (MQ=255)                                              gcgcCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCCAACCTTTGCCCgg                                                                                                          <  2:293270/147‑2 (MQ=255)                                                      tgtACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACcagcgacag                                                                                                >  7:234943/1‑149 (MQ=255)                                                       gtACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACcagcgacagc                                                                                               >  2:297116/1‑149 (MQ=255)                                                              aTCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAgcgc                                                                                         >  8:222949/1‑148 (MQ=255)                                                               tCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCa                                                                                       <  8:234943/149‑1 (MQ=255)                                                                   aaaCAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGccc                                                                                   >  8:264308/1‑149 (MQ=255)                                                                    aaCAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCt                                                                                  >  3:73026/1‑149 (MQ=255)                                                                          cGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCa                                                                            <  3:90951/149‑1 (MQ=255)                                                                                  tGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                    >  2:240401/1‑149 (MQ=255)                                                                                    gATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                    <  1:297116/147‑1 (MQ=255)                                                                                     aTCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTa                                                                 <  1:240401/149‑1 (MQ=255)                                                                                      tCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCTGGTATTCGGTAg                                                                <  3:221072/149‑1 (MQ=255)                                                                                        aaaGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAgg                                                               <  6:10967/148‑1 (MQ=255)                                                                                         aaGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGAGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAg                                                                           >  4:320246/1‑135 (MQ=255)                                                                                         aaGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGAGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAg                                                                           >  4:315172/1‑135 (MQ=255)                                                                                         aaGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGAGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAg                                                                           <  3:315172/135‑1 (MQ=255)                                                                                         aaGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGAGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAg                                                                           <  3:320246/135‑1 (MQ=255)                                                                                              tGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGcggcgg                                                         >  6:37437/1‑148 (MQ=255)                                                                                                 tGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAg                                                     >  3:256094/1‑149 (MQ=255)                                                                                                    cTACGTAGAAGACGATGCTGCGGCCCCTGTTGCCGCGCAGGAAATCCGGCGCATAGGGGGGGGGAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTgg                                                  <  8:84305/149‑1 (MQ=255)                                                                                                     tACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTggg                                                 >  3:271147/1‑149 (MQ=255)                                                                                                     tACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTggg                                                 >  3:77516/1‑149 (MQ=255)                                                                                                         tCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGtt                                             <  3:29422/149‑1 (MQ=255)                                                                                                         tCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGtt                                             <  4:271147/149‑1 (MQ=255)                                                                                                            aaGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAg                                          <  3:143681/149‑1 (MQ=255)                                                                                                                          gCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTcgc                            >  7:55494/1‑149 (MQ=255)                                                                                                                            ccAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCgcgc                          <  8:55494/149‑1 (MQ=255)                                                                                                                              aGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCtt                        <  6:174687/149‑1 (MQ=255)                                                                                                                               ggTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCtt                        >  1:154545/1‑148 (MQ=255)                                                                                                                                     cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTg                 >  7:86663/1‑149 (MQ=255)                                                                                                                                      gcgcAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTgg                                                  >  6:103060/1‑115 (MQ=255)                                                                                                                                           ggAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAAc           <  7:264308/149‑1 (MQ=255)                                                                                                                                            gAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAAcg          >  3:9052/1‑149 (MQ=255)                                                                                                                                               aTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga       <  7:222949/149‑1 (MQ=255)                                                                                                                                               aTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga       <  4:73026/149‑1 (MQ=255)                                                                                                                                               aTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGGTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga       <  8:302931/149‑1 (MQ=255)                                                                                                                                                tCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCgat      >  3:150810/1‑149 (MQ=255)                                                                                                                                                 ccGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCgat      >  1:313590/1‑148 (MQ=255)                                                                                                                                                    gcgcATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGatgatg  <  2:154545/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATG  >  NC_002947/735622‑735917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑