Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 770,542 G→A 11.3% V76I (GTC→ATC) ‡ PP_0662 → threonine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947770,5420GA11.3% 142.9 / 5.9 53V76I (GTC→ATC) ‡PP_0662threonine synthase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base A (3/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGGGCTTTATGTGCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTG  >  NC_002947/770394‑770683
                                                                                                                                                    |                                                                                                                                             
tGGCGGGCTTTATGTGCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTgg                                                                                                                                               >  1:100439/1‑149 (MQ=255)
        tttATGTGCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGa                                                                                                                                        >  2:128222/1‑148 (MQ=255)
           aTGTGCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAg                                                                                                                                     <  3:134076/148‑1 (MQ=255)
            tgtgCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAg                                                                                                                                     <  4:54029/147‑1 (MQ=255)
            tgtgCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAg                                                                                                                                     >  3:54029/1‑147 (MQ=255)
             gtgCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTa                                                                                                                                  >  8:136466/1‑149 (MQ=255)
                cccACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATcg                                                                                                                               >  6:65153/1‑149 (MQ=255)
                 ccACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATcgc                                                                                                                              <  5:282238/149‑1 (MQ=255)
                 ccACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATcgc                                                                                                                              >  7:234470/1‑149 (MQ=255)
                  cACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATcgcg                                                                                                                             >  4:10738/1‑149 (MQ=255)
                   aCCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACACTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCgg                                                                                                                            >  7:70334/1‑149 (MQ=255)
                     cTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGt                                                                                                                          <  1:128222/149‑1 (MQ=255)
                        ggTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCCGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTcc                                                                                                                       <  3:245292/149‑1 (MQ=255)
                               gtgtTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACcg                                                                                                                >  6:272704/1‑149 (MQ=255)
                                        ccACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgc                                                                                                       <  8:234470/149‑1 (MQ=255)
                                          acaGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgcgc                                                                                                     >  3:127446/1‑149 (MQ=255)
                                          acaGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgcgc                                                                                                     >  2:293883/1‑149 (MQ=255)
                                          acaGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgcgc                                                                                                     >  5:98466/1‑149 (MQ=255)
                                          acaGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgcgc                                                                                                     >  5:91584/1‑149 (MQ=255)
                                          acaGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTgcgc                                                                                                     <  5:272704/149‑1 (MQ=255)
                                             ggAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGt                                                                                                  <  2:100439/149‑1 (MQ=255)
                                              gAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGtt                                                                                                 <  7:136466/149‑1 (MQ=255)
                                                 aTTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTgca                                                                                              <  6:98466/149‑1 (MQ=255)
                                                  ttGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTgcag                                                                                             <  3:10738/149‑1 (MQ=255)
                                                            gTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGc                                                                                    >  7:241962/1‑148 (MQ=255)
                                                              cGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCa                                                                                  >  3:176589/1‑148 (MQ=255)
                                                                        cTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTg                                                                                      >  6:203484/1‑134 (MQ=255)
                                                                        cTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTg                                                                                      <  5:203484/134‑1 (MQ=255)
                                                                           cgacgaACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATc                                                                     <  5:65153/148‑1 (MQ=255)
                                                                               gaACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAg                                                                                                                                           >  1:258349/1‑74 (MQ=255)
                                                                               gaACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAg                                                                                                                                           <  2:258349/74‑1 (MQ=255)
                                                                               gaACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTg                                                                  >  5:274846/1‑147 (MQ=255)
                                                                                     gcctggcGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCtttt                                                          >  7:20062/1‑149 (MQ=255)
                                                                                            gggTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATggg                                                   <  6:91584/149‑1 (MQ=255)
                                                                                                  tGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTAcc                                             <  4:127446/149‑1 (MQ=255)
                                                                                                    gCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCg                                           <  1:293883/149‑1 (MQ=255)
                                                                                                     cACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCgg                                          >  2:210552/1‑149 (MQ=255)
                                                                                                     cACCGTTTGTCGGCGATACACTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGGTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCg                                           <  8:70334/148‑1 (MQ=255)
                                                                                                            tGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGtcctcc                                    >  5:134082/1‑148 (MQ=255)
                                                                                                            tGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGtcctcc                                    <  4:176589/148‑1 (MQ=255)
                                                                                                              tCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTAcc                                             >  2:146894/1‑137 (MQ=255)
                                                                                                              tCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTAcc                                             <  1:146894/137‑1 (MQ=255)
                                                                                                                 gCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGAc                              <  6:274846/149‑1 (MQ=255)
                                                                                                                 gCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGAc                              >  3:19454/1‑149 (MQ=255)
                                                                                                                         cTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCcgc                      >  5:54053/1‑149 (MQ=255)
                                                                                                                              cgaGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGt                 <  8:20062/149‑1 (MQ=255)
                                                                                                                                        cTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGgcgcg       >  8:292903/1‑149 (MQ=255)
                                                                                                                                           ctgTCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCtt    >  3:6942/3‑149 (MQ=255)
                                                                                                                                           ctgTCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCtt    >  3:2291/3‑149 (MQ=255)
                                                                                                                                            tgTCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAGCGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCt     >  5:76001/2‑147 (MQ=255)
                                                                                                                                            tgTCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAGCGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCt     <  6:76001/146‑1 (MQ=255)
                                                                                                                                            ggTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCttt   <  1:25413/149‑1 (MQ=255)
                                                                                                                                              tCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTg  <  4:6942/148‑1 (MQ=255)
                                                                                                                                              tCGCTGACCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTg  <  4:2291/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TGGCGGGCTTTATGTGCCCACCTCGGTGCCGGTGTTCAGCCCACAGGAAATTGCCAGCTGGTCGTGGTTGCCCTTCGACGAACTGGCCTGGCGGGTAATGGCACCGTTTGTCGGCGATACCCTCGACGAGCCTACGCTCAGGTCGCTGGTCAGCGACAGCTATCGCGGGTTCCGCCACCGCGGCATTGCGCCGTTGCAGCAGCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTG  >  NC_002947/770394‑770683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑