Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	845,808	A→C	6.4%	R67R (CGT→CGG)	PP_0728 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	845,808	0	A	C	6.4%	236.1 / 4.7	74	R67R (CGT→CGG)	PP_0728	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base C (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACGTGTCAGGTCGGAGAA  >  NC_002947/845660‑845954                                                                                                                                                     |                                                                                                                                                    ggAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCa                                                                                                                                                     >  8:76884/1‑149 (MQ=255)           cTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAgcgc                                                                                                                                           <  3:57185/149‑1 (MQ=255)              aTGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCCCGTGCAgcgttcg                                                                                                                                        >  6:113141/1‑145 (MQ=255)              aTGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAg                                                                                                                                        >  4:282390/1‑149 (MQ=255)                gCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGc                                                                                                                                       >  5:60017/1‑148 (MQ=255)                 cAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCCCGTGCAgcgttcgta                                                                                                                                      <  5:113141/148‑7 (MQ=255)                  aaCGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGaa                                                                                                                                    >  5:30694/1‑149 (MQ=255)                  aaCGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGa                                                                                                                                     >  3:223840/1‑148 (MQ=255)                          gAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAg                                                                                                                            >  8:233525/1‑149 (MQ=255)                           aaaTCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGc                                                                                                                           <  3:282390/149‑1 (MQ=255)                            aaTCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGc                                                                                                                           >  1:312172/1‑148 (MQ=255)                                  tGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACg                                                                                                                    >  4:5429/1‑149 (MQ=255)                                    gCCATCTCGCTACCGATACTATTGACCCATCAGGTCACATGATCGATGAAACCGGCAGATACTACACCCGCTGTATCCAGCGATTTAATTATTGCGTTACATACGAAAGCTGCACGGGCAGCGCCAGCGAATACTTCAga                                                                                                                           >  5:129045/1‑139 (MQ=255)                                    gCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGTCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCatc                                                                                                                           <  6:129045/140‑3 (MQ=255)                                    gCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGtt                                                                                                                  <  7:233525/149‑1 (MQ=255)                                    gCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGt                                                                                                                   <  3:5429/148‑1 (MQ=255)                                     ccATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTg                                                                                                                 <  5:40106/149‑1 (MQ=255)                                     ccATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTg                                                                                                                 <  6:30694/149‑1 (MQ=255)                                        tctcGCGACCGATACTATTGACACCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTgg                                                                                                                >  1:227757/1‑147 (MQ=255)                                         ctcGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGgcc‑g                                                                                                            >  4:177799/1‑149 (MQ=255)                                          tcGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGgcc‑gc                                                                                                           >  6:180047/1‑149 (MQ=255)                                                ccGATATTATTGACCCCTCCGTTCAAATGTTCGATGAACCCGGCATATACTCCCCCCGCTGTTTCATGGGTTTTAATTATTGCGGTACCTACGAACGCTGCCCGGGCAGCGCCAGCTAATACTTCTGCGACCACGTTGGCC‑GCCGATa                                                                                                      <  6:60017/148‑1 (MQ=255)                                                    tACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCa                                                                                                 >  7:249553/1‑149 (MQ=255)                                                         ttGACGCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACTGGCAGCGCCAGc                                                                                                                                       <  6:11666/107‑1 (MQ=255)                                                         ttGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCt                                                                                            >  5:250404/1‑149 (MQ=255)                                                            aCCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCa                                                                                          >  8:63354/1‑148 (MQ=255)                                                                     gTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGAGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGc                                                                                  >  4:209346/1‑147 (MQ=255)                                                                        acaTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATa                                                                                                      <  6:330774/124‑1 (MQ=255)                                                                        acaTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATa                                                                                                      >  5:330774/1‑124 (MQ=255)                                                                        acaTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGa                                                                             <  7:76884/149‑1 (MQ=255)                                                                        acaTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGa                                                                             <  5:271444/149‑1 (MQ=255)                                                                         caTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGc                                                                                                                                       >  4:139679/1‑91 (MQ=255)                                                                         caTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGc                                                                                                                                       <  3:139679/91‑1 (MQ=255)                                                                          aTGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACt                                                                           >  2:13073/1‑149 (MQ=255)                                                                           tGATCTATGAATCCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTc                                                                          <  2:146279/149‑1 (MQ=255)                                                                            gATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGa                                                                             >  4:18530/1‑145 (MQ=255)                                                                            gATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGa                                                                             <  3:18530/145‑1 (MQ=255)                                                                            gATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCg                                                                         >  2:105928/1‑149 (MQ=255)                                                                             aTCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGc                                                                        >  6:95963/1‑149 (MQ=255)                                                                                gATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGcccc                                                                     >  4:281506/1‑149 (MQ=255)                                                                                gATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGcccc                                                                     >  5:189030/1‑149 (MQ=255)                                                                                 aTGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTcc                                                                                             >  1:334969/1‑124 (MQ=255)                                                                                 aTGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTcc                                                                                             <  2:334969/124‑1 (MQ=255)                                                                                 aTGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGcccc                                                                     >  3:298264/1‑148 (MQ=255)                                                                                  tGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGa                                                                   <  1:13073/149‑1 (MQ=255)                                                                                      aCCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGtt                                                               <  4:223840/149‑1 (MQ=255)                                                                                          gCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCt                                                           >  8:17170/1‑149 (MQ=255)                                                                                             gATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGc                                                        <  8:249553/149‑1 (MQ=255)                                                                                              aTACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGcc                                                       <  3:38773/149‑1 (MQ=255)                                                                                                    cccccGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTaaa                                                 >  3:289035/1‑149 (MQ=255)                                                                                                    cccccGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCAAGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTaaa                                                 >  2:203756/1‑149 (MQ=255)                                                                                                       ccGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAGGGACTCGCCCCGAGGTTGCCTCGCCATTAAActc                                              >  6:272058/1‑149 (MQ=255)                                                                                                        cGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAAct                                               >  4:309333/1‑147 (MQ=255)                                                                                                        cGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAAct                                               <  3:309333/147‑1 (MQ=255)                                                                                                         gCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTg                                            <  3:177799/149‑1 (MQ=255)                                                                                                          cTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTg                                            >  5:67775/1‑148 (MQ=255)                                                                                                             tttCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCAc                                         <  5:180047/148‑1 (MQ=255)                                                                                                                 cAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGt                                    <  6:250404/149‑1 (MQ=255)                                                                                                                 cAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGt                                    <  3:209346/149‑1 (MQ=255)                                                                                                                   gCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCATCGACCACGTTGGCCTTTCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTc                                   <  1:105928/149‑1 (MQ=255)                                                                                                                   gCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCa                                  <  6:189030/149‑1 (MQ=255)                                                                                                                           attattGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATAc                           <  3:281506/148‑1 (MQ=255)                                                                                                                            ttattGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTc                         <  5:87915/149‑1 (MQ=255)                                                                                                                             tattGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCa                                  >  2:290615/1‑139 (MQ=255)                                                                                                                               ttGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCgg                       <  2:227757/148‑1 (MQ=255)                                                                                                                               ttGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCgg                       <  2:312172/148‑1 (MQ=255)                                                                                                                                tGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGt                      <  1:203756/148‑1 (MQ=255)                                                                                                                                 gCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCgg                       >  2:19233/1‑146 (MQ=255)                                                                                                                                   ggTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAGGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGAc                  <  5:272058/149‑1 (MQ=255)                                                                                                                                        cTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACGTGTc             <  5:95963/149‑1 (MQ=255)                                                                                                                                         tACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACgtgt              >  7:21318/1‑147 (MQ=255)                                                                                                                                            tggcGCTGCCCGTGCAGCGTCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGt                      >  6:42063/4‑136 (MQ=255)                                                                                                                                            tggcGCTGCCCGTGCAGCGTCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGt                      <  5:42063/133‑1 (MQ=255)                                                                                                                                                gCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACGTGTCAGGTCGga     <  7:17170/149‑1 (MQ=255)                                                                                                                                                    cACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACGTGTCAGGTCGGagaa  <  4:289035/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGAAATCGTCCTTATGCAACGTCTGGAAATCGCTGGCCATCTCGCGACCGATACTATTGACCCCTCCGGTCACATGATCGATGAAACCGGCAGATACTACCCCCGCTGTTTCCAGCGATTTAATTATTGCGGTACCTACGAACGCTGCACGGGCAGCGCCAGCGAATACTTCAGCGACCACGTTGGCC‑GCCGATAGAGCATTCCTCATACGTTGCAAGGACTCGCCCCGAGGTTGCCTCGCCATTAAACTCTGCACCTTGTCAGTAATACTCGGTAGACGTGTCAGGTCGGAGAA  >  NC_002947/845660‑845954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑