Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	873,418	G→A	6.9%	G18S (GGC→AGC)	PP_5457 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	873,418	0	G	A	6.9%	171.3 / 2.5	59	G18S (GGC→AGC)	PP_5457	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CCGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGC  >  NC_002947/873270‑873560                                                                                                                                                       |                                                                                                                                               ccGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTgg‑‑cag                                                                                                                                                >  5:197193/1‑149 (MQ=255)   gAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTgg‑‑caggc                                                                                                                                              <  5:36043/149‑1 (MQ=255)       aTTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGc                                                                                                                                          <  7:143068/149‑1 (MQ=255)                tgtgCTGTCAGCCTGTGGTGTGCATTCTGCATGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGTCGCAAATGCTGT‑‑CAAGCATGCGTGCCAGca                                                                                                                                 <  4:63596/149‑2 (MQ=255)                     tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAggg                                                                                                                             >  1:44685/1‑148 (MQ=255)                       tCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGAt                                                                                                                          >  5:132125/1‑149 (MQ=255)                                ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTaca                                                                                                                 >  6:304901/1‑149 (MQ=255)                                   gtgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGt                                                                                                              >  4:235035/1‑149 (MQ=255)                                        ttCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCg                                                                                                         >  3:293137/1‑149 (MQ=255)                                          cTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCgg                                                                                                        <  8:206513/148‑1 (MQ=255)                                            gCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCg                                                                                                     >  4:114102/1‑149 (MQ=255)                                               ggTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGa                                                                                                  <  3:67057/149‑1 (MQ=255)                                                 tGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                <  6:197193/149‑1 (MQ=255)                                                   cATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCtt                                                                                              >  4:112176/1‑149 (MQ=255)                                                     tGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                <  7:90110/145‑1 (MQ=255)                                                     tGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                >  8:90110/1‑145 (MQ=255)                                                       gTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCg                                                                                          >  7:197829/1‑149 (MQ=255)                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTTG‑‑CAGGCATGCTTGCCAGCGAg                                                                                                                               <  5:261339/111‑1 (MQ=255)                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAg                                                                                                                               >  6:261339/1‑111 (MQ=255)                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                      >  2:7054/1‑136 (MQ=255)                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                      <  1:7054/136‑1 (MQ=255)                                                          aCGAAGGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACATGAAAGCCTGCTTGATGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGCTCGGCCGTGACCTTGTCGGTg                                                                                       >  3:255700/1‑149 (MQ=255)                                                                   tGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCg                                                                              <  5:304901/149‑1 (MQ=255)                                                                    gCTTGAAGTCCGCCGCGAGGACTACACCTTATGCTCCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGTCCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCgg                                                                                <  1:102145/148‑1 (MQ=255)                                                                     cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGa                                                                             <  3:235035/148‑1 (MQ=255)                                                                     cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                            <  8:197829/149‑1 (MQ=255)                                                                       tGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                            <  6:108589/147‑1 (MQ=255)                                                                       tGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                            >  5:108589/1‑147 (MQ=255)                                                                          aGTCCGCCGCGAGGACGACACCTGATTCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CATGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTTcc                                                                       <  4:255700/149‑1 (MQ=255)                                                                          aGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGGCGCCGGCGAGCTGcc                                                                       <  4:293137/149‑1 (MQ=255)                                                                            tCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCt                                                                     >  1:188979/1‑149 (MQ=255)                                                                             ccgccgCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAg                                                                                                                               <  5:1293/90‑1 (MQ=255)                                                                             ccgccgCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAg                                                                                                                               >  6:1293/1‑90 (MQ=255)                                                                               gccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTg                                                                   >  8:274806/1‑148 (MQ=255)                                                                               gccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGc                                                                  <  6:132125/149‑1 (MQ=255)                                                                                       gacgacACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCctg                                                          <  8:110733/149‑1 (MQ=255)                                                                                           acacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                      <  7:274806/149‑1 (MQ=255)                                                                                            cagcTGATGGCGCGTATCTTCGAACCGGAAAGCCTGCTTGAGGCGCTAATGCTGG‑‑CAGGCATGCTTGCGAGCGAGGGGATGGATGTTCACGTGGTCGGCCGTGTGCTTGTCGGTTCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                      <  7:141248/145‑1 (MQ=255)                                                                                             acCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                    <  3:112176/149‑1 (MQ=255)                                                                                                 gATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCTGCCgg                                                                                <  1:314538/117‑1 (MQ=255)                                                                                                 gATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCAGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCgg                                                                                >  2:314538/1‑117 (MQ=255)                                                                                                       gCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTgg                                          <  2:188979/149‑1 (MQ=255)                                                                                                            tCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGccc                                                                      <  4:178013/116‑1 (MQ=255)                                                                                                            tCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGccc                                                                      >  3:178013/1‑116 (MQ=255)                                                                                                               aCGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                  >  4:14463/1‑149 (MQ=255)                                                                                                                cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgatga                                   <  5:189287/147‑1 (MQ=255)                                                                                                                cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgatg                                    <  3:114102/146‑1 (MQ=255)                                                                                                                 gAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                  >  1:45764/1‑147 (MQ=255)                                                                                                                   aCCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAgg                              >  8:228354/1‑149 (MQ=255)                                                                                                                       gAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCg                           >  7:31925/1‑148 (MQ=255)                                                                                                                         aaGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAAt                        <  2:45764/149‑1 (MQ=255)                                                                                                                                    aGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAg             >  3:50605/1‑149 (MQ=255)                                                                                                                                        gcAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGc                                         <  8:293291/117‑1 (MQ=255)                                                                                                                                        gcAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGa         >  1:219595/1‑149 (MQ=255)                                                                                                                                          aaaTGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgat                                     >  6:65187/1‑119 (MQ=255)                                                                                                                                          aaaTGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgat                                     <  5:65187/119‑1 (MQ=255)                                                                                                                                           ctcgCTGG‑‑CAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc       >  6:302700/4‑148 (MQ=255)                                                                                                                                            tcgCTGG‑‑CAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGa     >  4:144611/3‑149 (MQ=255)                                                                                                                                               cTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATg   <  7:228354/148‑1 (MQ=255)                                                                                                                                               cTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGc  <  3:14463/149‑1 (MQ=255)                                                                                                                                               cTGG‑‑CAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGc  <  5:302700/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                               CCGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGG‑‑CAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGC  >  NC_002947/873270‑873560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑