Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,600 G→A 10.0% S4341L (TCG→TTG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,6000GA10.0% 163.7 / 5.5 59S4341L (TCG→TTG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base A (3/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

CAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCT  >  NC_002947/932455‑932748
                                                                                                                                                 |                                                                                                                                                    
cAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGaca                                                                                                                                                   >  5:206899/1‑149 (MQ=255)
 aGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGacac                                                                                                                                                  >  1:159003/1‑149 (MQ=255)
  gCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACg                                                                                                                                                 >  7:102049/1‑149 (MQ=255)
    aCGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGtt                                                                                                                                               <  8:201920/149‑1 (MQ=255)
     cGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTg                                                                                                                                              <  6:206899/149‑1 (MQ=255)
       gTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGcc                                                                                                                                            <  6:55953/149‑1 (MQ=255)
        tACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCg                                                                                                                                           <  7:2794/149‑1 (MQ=255)
        tACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCg                                                                                                                                           >  8:154812/1‑149 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGac                                                                                                                                                    <  8:64859/136‑1 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGac                                                                                                                                                    >  7:64859/1‑136 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGccg                                                                                                                                       <  6:59340/149‑3 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGccg                                                                                                                                       <  8:170020/149‑3 (MQ=255)
               cGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                                     <  6:306737/148‑2 (MQ=255)
                 gTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCg                                                                                                                                  >  3:242441/1‑149 (MQ=255)
                 gTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGGGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCg                                                                                                                                  >  3:48120/1‑149 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTgg                                                                                                                              <  7:154812/147‑1 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCg                                                                                                                            <  3:285150/149‑1 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCg                                                                                                                            >  3:295368/1‑149 (MQ=255)
                               aCGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCt                                                                                                                     <  6:138540/148‑1 (MQ=255)
                                       gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGGATCt                                                                                                            >  4:45063/1‑149 (MQ=255)
                                       gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCAGCTGCGGTGAAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGGCGGTGGCGGTCACCTGCAGTATCt                                                                                                            >  3:192447/1‑149 (MQ=255)
                                        ccTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTg                                                                                                           <  3:222880/149‑1 (MQ=255)
                                         cTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGTCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTgg                                                                                                          <  1:223578/149‑1 (MQ=255)
                                          tGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                         >  3:43071/1‑149 (MQ=255)
                                           gCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCGGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                         >  1:108962/1‑148 (MQ=255)
                                           gCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                         >  8:62732/1‑148 (MQ=255)
                                            cTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCa                                                                                                       <  7:131969/149‑1 (MQ=255)
                                             ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                                      >  2:222020/1‑149 (MQ=255)
                                                  cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                         >  2:54024/1‑141 (MQ=255)
                                                  cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                         <  1:54024/141‑1 (MQ=255)
                                                   aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                                <  7:36132/149‑1 (MQ=255)
                                                           tCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACt                                                                                         <  8:102049/148‑1 (MQ=255)
                                                             aGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGtt                                                                                      >  2:229951/1‑149 (MQ=255)
                                                                    cgcCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGt                                                                                >  8:227519/1‑148 (MQ=255)
                                                                            gCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGaa                                                                       <  4:43071/149‑1 (MQ=255)
                                                                                    aGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGt                                                               >  8:236383/1‑149 (MQ=255)
                                                                                     gCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGtt                                                              <  3:23964/149‑1 (MQ=255)
                                                                                      ccAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                             <  7:62732/149‑1 (MQ=255)
                                                                                         ggTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCa                                                          <  1:229951/149‑1 (MQ=255)
                                                                                              gCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCgg                                                      <  4:242441/148‑1 (MQ=255)
                                                                                                cACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCACCCCCTTGCCGGCTGCGTGGGGGGCGGTGCCCGGCAGGATCTGGCCATTGGTGTGGGGACTGTTGAAGGTGGCCTGGCAGGTGCCGTTTCCCCCGGGTa                                                   >  1:80428/1‑149 (MQ=255)
                                                                                                     cggccggcggcGTTGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGTCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCt                                              <  4:48120/149‑1 (MQ=255)
                                                                                                        ccggcggcGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACTCGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCAGTGTTCAAGGTGACCTGGAAGGTGCCGTTGGCACCGGCTACCGCtgtg                                           <  4:192447/149‑1 (MQ=255)
                                                                                                        ccggcggcGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCtgtg                                           >  3:285124/1‑149 (MQ=255)
                                                                                                              gcgTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGc                                     <  4:285124/149‑1 (MQ=255)
                                                                                                                  ggtgTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGt                                 >  8:89067/1‑149 (MQ=255)
                                                                                                                   gtgtCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGtt                                <  5:298286/149‑1 (MQ=255)
                                                                                                                                tgtAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATcg                   >  4:93606/1‑149 (MQ=255)
                                                                                                                                     ggTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcac              >  4:112773/1‑149 (MQ=255)
                                                                                                                                     ggTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcac              >  1:146386/1‑149 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                             >  4:67420/1‑101 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                             <  3:67420/101‑1 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacg             <  2:108962/149‑1 (MQ=255)
                                                                                                                                         cagccggcAACGTGTCGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggt           >  3:156059/3‑148 (MQ=255)
                                                                                                                                          agccggcAACGTGTCGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtca         >  6:28915/2‑149 (MQ=255)
                                                                                                                                           gccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcac        >  3:317289/1‑149 (MQ=255)
                                                                                                                                            ccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacg       >  6:314688/1‑149 (MQ=255)
                                                                                                                                            ccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGGCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacg       <  1:222020/149‑1 (MQ=255)
                                                                                                                                             cggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacgg      <  7:89067/149‑1 (MQ=255)
                                                                                                                                                 gACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGtgct  <  2:80428/149‑1 (MQ=255)
                                                                                                                                                 gACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGtgct  <  2:159003/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCT  >  NC_002947/932455‑932748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑