Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,603 A→G 9.1% V4340A (GTG→GCG) ‡ PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,6030AG 9.1% 146.9 / 5.4 57V4340A (GTG→GCG) ‡PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base G (2/3);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

CAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCC  >  NC_002947/932455‑932751
                                                                                                                                                    |                                                                                                                                                    
cAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGaca                                                                                                                                                      >  5:206899/1‑149 (MQ=255)
 aGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGacac                                                                                                                                                     >  1:159003/1‑149 (MQ=255)
  gCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACg                                                                                                                                                    >  7:102049/1‑149 (MQ=255)
    aCGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGtt                                                                                                                                                  <  8:201920/149‑1 (MQ=255)
     cGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTg                                                                                                                                                 <  6:206899/149‑1 (MQ=255)
       gTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGcc                                                                                                                                               <  6:55953/149‑1 (MQ=255)
        tACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCg                                                                                                                                              >  8:154812/1‑149 (MQ=255)
        tACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCg                                                                                                                                              <  7:2794/149‑1 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGccg                                                                                                                                          <  6:59340/149‑3 (MQ=255)
            gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGccg                                                                                                                                          <  8:170020/149‑3 (MQ=255)
               cGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                                        <  6:306737/148‑2 (MQ=255)
                 gTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCg                                                                                                                                     >  3:242441/1‑149 (MQ=255)
                 gTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGGGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCg                                                                                                                                     >  3:48120/1‑149 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTgg                                                                                                                                 <  7:154812/147‑1 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCg                                                                                                                               >  3:295368/1‑149 (MQ=255)
                       gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCg                                                                                                                               <  3:285150/149‑1 (MQ=255)
                               aCGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCt                                                                                                                        <  6:138540/148‑1 (MQ=255)
                                       gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGGATCt                                                                                                               >  4:45063/1‑149 (MQ=255)
                                       gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCAGCTGCGGTGAAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGGCGGTGGCGGTCACCTGCAGTATCt                                                                                                               >  3:192447/1‑149 (MQ=255)
                                        ccTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTg                                                                                                              <  3:222880/149‑1 (MQ=255)
                                         cTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGTCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTgg                                                                                                             <  1:223578/149‑1 (MQ=255)
                                          tGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                            >  3:43071/1‑149 (MQ=255)
                                           gCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCGGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                            >  1:108962/1‑148 (MQ=255)
                                           gCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                            >  8:62732/1‑148 (MQ=255)
                                            cTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCa                                                                                                          <  7:131969/149‑1 (MQ=255)
                                             ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                                         >  2:222020/1‑149 (MQ=255)
                                                  cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                            >  2:54024/1‑141 (MQ=255)
                                                  cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                            <  1:54024/141‑1 (MQ=255)
                                                   aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                                   <  7:36132/149‑1 (MQ=255)
                                                           tCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACt                                                                                            <  8:102049/148‑1 (MQ=255)
                                                             aGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGtt                                                                                         >  2:229951/1‑149 (MQ=255)
                                                                    cgcCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGt                                                                                   >  8:227519/1‑148 (MQ=255)
                                                                            gCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGaa                                                                          <  4:43071/149‑1 (MQ=255)
                                                                                    aGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGt                                                                  >  8:236383/1‑149 (MQ=255)
                                                                                     gCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGtt                                                                 <  3:23964/149‑1 (MQ=255)
                                                                                      ccAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                                <  7:62732/149‑1 (MQ=255)
                                                                                         ggTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCa                                                             <  1:229951/149‑1 (MQ=255)
                                                                                              gCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCgg                                                         <  4:242441/148‑1 (MQ=255)
                                                                                                cACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCACCCCCTTGCCGGCTGCGTGGGGGGCGGTGCCCGGCAGGATCTGGCCATTGGTGTGGGGACTGTTGAAGGTGGCCTGGCAGGTGCCGTTTCCCCCGGGTa                                                      >  1:80428/1‑149 (MQ=255)
                                                                                                     cggccggcggcGTTGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGTCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCt                                                 <  4:48120/149‑1 (MQ=255)
                                                                                                        ccggcggcGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACTCGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCAGTGTTCAAGGTGACCTGGAAGGTGCCGTTGGCACCGGCTACCGCtgtg                                              <  4:192447/149‑1 (MQ=255)
                                                                                                        ccggcggcGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCtgtg                                              >  3:285124/1‑149 (MQ=255)
                                                                                                              gcgTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGc                                        <  4:285124/149‑1 (MQ=255)
                                                                                                                  ggtgTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGt                                    >  8:89067/1‑149 (MQ=255)
                                                                                                                   gtgtCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGtt                                   <  5:298286/149‑1 (MQ=255)
                                                                                                                                tgtAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATcg                      >  4:93606/1‑149 (MQ=255)
                                                                                                                                     ggTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcac                 >  1:146386/1‑149 (MQ=255)
                                                                                                                                     ggTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcac                 >  4:112773/1‑149 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                                >  4:67420/1‑101 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                                <  3:67420/101‑1 (MQ=255)
                                                                                                                                      gTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacg                <  2:108962/149‑1 (MQ=255)
                                                                                                                                         cagccggcAACGTGTCGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggt              >  3:156059/3‑148 (MQ=255)
                                                                                                                                          agccggcAACGTGTCGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtca            >  6:28915/2‑149 (MQ=255)
                                                                                                                                           gccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcac           >  3:317289/1‑149 (MQ=255)
                                                                                                                                            ccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacg          >  6:314688/1‑149 (MQ=255)
                                                                                                                                            ccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGGCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacg          <  1:222020/149‑1 (MQ=255)
                                                                                                                                             cggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacgg         <  7:89067/149‑1 (MQ=255)
                                                                                                                                                 gACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGtgct     <  2:80428/149‑1 (MQ=255)
                                                                                                                                                 gACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGtgct     <  2:159003/149‑1 (MQ=255)
                                                                                                                                                   cacGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGtgctgc   <  7:227519/149‑1 (MQ=255)
                                                                                                                                                    acGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGCCCTGGAAGGTGCCGTTGCCACCGGATACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGcc  >  5:251937/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCC  >  NC_002947/932455‑932751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑