Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 970,692 T→G 8.9% F181V (TTC→GTC)  tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947970,6920TG 8.9% 104.6 / 2.7 43F181V (TTC→GTC) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base G (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GCGTGACCTGGGCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTATCTGCC  >  NC_002947/970544‑970840
                                                                                                                                                    |                                                                                                                                                    
gCGTGACCTGGGCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGt                                                                                                                                                      >  6:160923/1‑149 (MQ=255)
        tGGGCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAAAGCCCATGACGACAACACCGCCGCGCTGTTCGGCAtt                                                                                                                                              >  3:194201/1‑149 (MQ=255)
        tGGGCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCAAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGGCCAGCGCGCGAAGAACCCCCAAGACGACAACACCGCCGCGCTGTTCGGCAtt                                                                                                                                              >  6:228617/1‑149 (MQ=255)
          ggCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCAtt                                                                                                                                              <  2:150628/147‑1 (MQ=255)
                   gTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGggcgg                                                                                                                                   <  2:190830/149‑1 (MQ=255)
                    tGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGggcggc                                                                                                                                  <  5:160923/149‑1 (MQ=255)
                               ttCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGggcggc                                                                                                                                  <  1:244659/138‑1 (MQ=255)
                               ttCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGggcggc                                                                                                                                  >  2:244659/1‑138 (MQ=255)
                                   acgaGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCCCGCGAAGAACCCCCATGCCCACAACACCCCAGCGCTGTTCGGCATTGTACAGGGCCGCATGTAACACGCCCtt                                                                                                                   >  6:26192/1‑148 (MQ=255)
                                       gTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCCCCCGGCGAAGAACCCCCAAGCCCCCAACACCCCCCCCCTGTTCGGGCATGTCCAGGGCCGGCTGTAACCGGCCCAgcgc                                                                                                                >  6:230958/1‑148 (MQ=255)
                                                    ccGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCCTGCCGACAACACCGCAGCGCTGTTGGGCATTGTGCAGGGCGGCATGTACCAGGACCAGCGCATGCGCGCGCggg                                                                                                   >  4:12571/1‑148 (MQ=255)
                                                            gCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTTGGCCCAGCGCTCGAAGAACCCCCAAGCCCACAACACCCCAGCGCTGGTCGGCATTGTACAGGGCGGCATGTACCAGGACCAGCGCATGCGCTCCCAGGAAGGCCTg                                                                                           >  6:28581/1‑148 (MQ=255)
                                                            gCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGTCCCAGCGCTCGAAGACCGCCCATGCCGACATCACCGCAGCGCTGTGCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTgg                                                                                          <  8:106/149‑1 (MQ=255)
                                                            gCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCAATCCGCCAACACCCCACCGCTGGGCGGCCTTGTACAGGGCGGCCTGTACCACGCCCAGCGCAAGCGCCAGCGGGAAGGGCTg                                                                                           >  6:23601/1‑148 (MQ=255)
                                                                        acgcacCTCCATGGAGCTGTCGCTGCGCGGGGCCCCCCCCGCGAAGAACCCCCCAGCCGCCAACACCCCCGCGCGGTTGGGCATTGTCCAGGGGGGCATGTACCCGGACCAGCGCAAGGCCTCGCTGGAAGGCCTGGAAAACATCGGtt                                                                              >  8:110397/1‑149 (MQ=255)
                                                                          gcacCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTc                                                                            >  5:346747/1‑149 (MQ=255)
                                                                          gcacCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTc                                                                            <  5:26192/149‑1 (MQ=255)
                                                                            acCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGa                                                                          <  5:228617/149‑1 (MQ=255)
                                                                                           ttcgagcgctgGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTgg                                                           >  1:147779/6‑149 (MQ=255)
                                                                                           tCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCCTGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTgg                                                           >  5:199506/1‑149 (MQ=255)
                                                                                           tCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTg                                                            <  5:230958/148‑1 (MQ=255)
                                                                                                 cgctgGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTggcggc                                                       <  3:12571/147‑1 (MQ=255)
                                                                                                        cccAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTg                                              <  2:147779/149‑1 (MQ=255)
                                                                                                        cccAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTg                                              <  2:256123/149‑1 (MQ=255)
                                                                                                           aGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGc                                           >  8:112161/1‑149 (MQ=255)
                                                                                                           aGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGc                                           >  6:78508/1‑149 (MQ=255)
                                                                                                             cgcTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGa                                         <  7:112161/149‑1 (MQ=255)
                                                                                                               cTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGc                                       <  5:78508/149‑1 (MQ=255)
                                                                                                                tCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGcc                                      >  8:308185/1‑149 (MQ=255)
                                                                                                                tCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGcc                                      >  6:85824/1‑149 (MQ=255)
                                                                                                                tCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGcc                                      >  3:283876/1‑149 (MQ=255)
                                                                                                                         gCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACg                              >  4:283437/1‑148 (MQ=255)
                                                                                                                           ccATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTggc                                                          >  6:173488/1‑118 (MQ=255)
                                                                                                                           ccATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTggc                                                          <  5:173488/118‑1 (MQ=255)
                                                                                                                           ccATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGaaa                           >  3:89210/1‑149 (MQ=255)
                                                                                                                              tGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGa                        >  4:103145/1‑149 (MQ=255)
                                                                                                                                 cGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATc                      >  6:88459/1‑148 (MQ=255)
                                                                                                                                     aacaCCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCAGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGt                 >  2:305518/1‑149 (MQ=255)
                                                                                                                                       cacCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGc               >  3:240798/1‑149 (MQ=255)
                                                                                                                                          cGCAGCGCTGTTCGTCATTGTCCAGGGCGGCATGTACCAGTACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGTCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTgg            <  5:333969/149‑1 (MQ=255)
                                                                                                                                          cGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTgg            <  3:103145/149‑1 (MQ=255)
                                                                                                                                          cGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTgg            <  7:221703/149‑1 (MQ=255)
                                                                                                                                               cgcTGGTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTAt       <  5:23601/149‑1 (MQ=255)
                                                                                                                                               cgcTGGGCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTAt       <  5:28581/149‑1 (MQ=255)
                                                                                                                                                    ttCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTATCTg    <  5:85824/147‑1 (MQ=255)
                                                                                                                                                    ttCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTATCTGcc  >  3:238035/1‑149 (MQ=255)
                                                                                                                                                    ttCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTATCTGc   <  4:194201/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTGACCTGGGCTCGGACGTGGTAATGATCTTCGACGAGTGCACCCCGTACCCGGCCGAGCACGATGTGGCACGCACCTCCATGGAGCTGTCGCTGCGCTGGGCCCAGCGCTCGAAGAACGCCCATGCCGACAACACCGCAGCGCTGTTCGGCATTGTCCAGGGCGGCATGTACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGAAAACATCGGTTTCGACGGCCTGGCCATTGGCGGCCTGTCGGTGGGCGAGCCCAAGCACGAAATGATCAAGGTGCTGGATTATCTGCC  >  NC_002947/970544‑970840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑