Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,008,233 G→C 7.5% P172R (CCG→CGG)  yehX ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,008,2330GC 7.5% 156.3 / 2.2 51P172R (CCG→CGG) yehXABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGCAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTGATC  >  NC_002947/1008086‑1008376
                                                                                                                                                   |                                                                                                                                               
aGCTGCAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCgg                                                                                                                                                >  5:40785/1‑149 (MQ=255)
aGCTGCAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCgg                                                                                                                                                >  8:81920/1‑149 (MQ=255)
 gCTGCAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCGTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCggg                                                                                                                                               >  4:326051/1‑149 (MQ=255)
     cAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTc                                                                                                                                             <  3:291706/147‑1 (MQ=255)
         aGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTCGACCCGATCAACcgc                                                                                                                                       <  3:80402/149‑1 (MQ=255)
           cTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCCGGTCGACCgcgc                                                                                                                                     >  3:220219/1‑149 (MQ=255)
               ccGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCCTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGaa                                                                                                                                 <  3:326051/149‑1 (MQ=255)
                cGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACAATGATTACCGTCTTGTTCAGCGACCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAAc                                                                                                                                >  8:299121/1‑149 (MQ=255)
                     aggAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTc                                                                                                                           <  5:6192/148‑1 (MQ=255)
                              gCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCTGGTCGACCGCGCCGAACGGCTCGTCCATCaa                                                                                                                  <  1:123890/149‑1 (MQ=255)
                                 aTCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAg                                                                                                               >  6:58694/1‑149 (MQ=255)
                                  tCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAg                                                                                                               >  8:58035/1‑148 (MQ=255)
                                         gCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGt                                                                                                        <  7:191057/148‑1 (MQ=255)
                                            cAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCAt                                                                                                    <  6:126654/149‑1 (MQ=255)
                                              gTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAAc                                                                                                                 <  3:224175/134‑1 (MQ=255)
                                              gTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAAc                                                                                                                 >  4:224175/1‑134 (MQ=255)
                                               tttGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATctg                                                                                                 >  7:110608/1‑149 (MQ=255)
                                                       cTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTTCATCAACAGCACGGGTGCATCTGCTGCCAgg                                                                                         <  1:36433/149‑1 (MQ=255)
                                                        ttCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCAt                                                                                                                     <  6:90794/120‑1 (MQ=255)
                                                          cgtcgATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGgcg                                                                                      >  6:319620/1‑149 (MQ=255)
                                                            tcgATGTCGTGGCTGACGATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGcgcg                                                                                    <  7:299121/149‑1 (MQ=255)
                                                              gATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCCACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGaa                                                                                  <  7:81920/149‑1 (MQ=255)
                                                               aTGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAAt                                                                                 <  6:40785/149‑1 (MQ=255)
                                                                 gTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCa                                                                               >  6:317808/1‑149 (MQ=255)
                                                                  tCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCAc                                                                              <  5:58694/149‑1 (MQ=255)
                                                                    gTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACg                                                                             <  5:319620/148‑1 (MQ=255)
                                                                       gCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGa                                                                         >  4:270651/1‑149 (MQ=255)
                                                                          gACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATAc                                                                      >  4:118553/1‑149 (MQ=255)
                                                                           aCCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACg                                                                     >  6:63877/1‑149 (MQ=255)
                                                                                gATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGtt                                                                >  4:120540/1‑149 (MQ=255)
                                                                                   tACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGACCGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCt                                                             >  8:80559/1‑149 (MQ=255)
                                                                                    aCCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCt                                                             <  5:317808/148‑1 (MQ=255)
                                                                                      cGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGCGCGCGAATCACGCCGATACGCTGTTGCTGgc                                                          >  6:194893/1‑149 (MQ=255)
                                                                                            gTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTGTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCa                                                     <  7:23314/148‑1 (MQ=255)
                                                                                                  cgcCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCt                                              >  1:160085/1‑149 (MQ=255)
                                                                                                            aTTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTa                                    <  7:58035/149‑1 (MQ=255)
                                                                                                              ttCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGc                                  >  7:303281/1‑149 (MQ=255)
                                                                                                                 gaagaaCTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGtt                               >  4:301272/1‑149 (MQ=255)
                                                                                                                 gaagaaCTCGTTCTGGATCATCTCGCGGTTGATCGCGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGAGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGtt                               >  3:110527/1‑149 (MQ=255)
                                                                                                                   agaaCTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTg                              >  7:22878/1‑148 (MQ=255)
                                                                                                                   agaaCTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGc                             <  3:301272/149‑1 (MQ=255)
                                                                                                                     aaCTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAg                           >  3:269402/1‑149 (MQ=255)
                                                                                                                      aCTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAgg                          <  5:63877/149‑1 (MQ=255)
                                                                                                                             cTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCt                   >  2:36106/1‑149 (MQ=255)
                                                                                                                                gATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCTCGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTgg                <  7:212259/149‑1 (MQ=255)
                                                                                                                                 atcatcTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTggg               >  4:126382/1‑149 (MQ=255)
                                                                                                                                   ttcggcgcgGTCGACCCGATCAACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCtt                  >  5:254617/6‑144 (MQ=255)
                                                                                                                                   ttcggcgcgGTCGACCCGATCAACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCtt                  <  6:254617/139‑1 (MQ=255)
                                                                                                                                      ctcGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTc            >  3:130587/1‑147 (MQ=255)
                                                                                                                                         gcgGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGa          <  4:110527/146‑1 (MQ=255)
                                                                                                                                           ggTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTg     <  3:270651/149‑1 (MQ=255)
                                                                                                                                           ggTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTg     <  8:22878/149‑1 (MQ=255)
                                                                                                                                            gTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTGa    >  2:253115/1‑149 (MQ=255)
                                                                                                                                              tGATCGGGGCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTGATc  <  1:219972/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AGCTGCAACAGCTTGCCGGCACGGAAAATGGCAATCTTGTCGCCCAGTTTGATCGCTTCGTCGATGTCGTGGCTGACCATGATTACCGTCTTGTTCAGCGCCCGCTGCATTTCGAAGAACTCGTTCTGGATCATCTCGCGGTTGATCGGGTCGACCGCGCCGAACGGCTCGTCCATCAACAGCACGGGTGCATCTGCTGCCAGGGCGCGAATCACGCCGATACGCTGTTGCTGGCCGCCAGACAGCTCGCGTGGGTAGCGTTGCAGGTACTGCTTGGGTTCGAGCTTGATC  >  NC_002947/1008086‑1008376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑