Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,226,710	T→A	6.4%	intergenic (‑220/‑111)	gltI ← / → PP_1072	glutamate/aspartate ABC transporter substrate‑binding protein/leucine‑rich repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,226,710	0	T	A	6.4%	188.0 / 2.8	62	intergenic (‑220/‑111)	gltI/PP_1072	glutamate/aspartate ABC transporter substrate‑binding protein/leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (33/25);  new base A (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

TGCATTCGGCTACCAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATCGATA  >  NC_002947/1226567‑1226857                                                                                                                                                  |                                                                                                                                                    tGCATTCGGCTACCAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGaa                                                                                                                                                <  6:173224/149‑1 (MQ=255)    aTTCGGCTACCAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACg                                                                                                                                              >  1:274527/1‑148 (MQ=255)    aTTCGGCTACCAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGATAACCGAACga                                                                                                                                             <  5:46607/149‑2 (MQ=255)             ccAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTCTCGACCTTAAACCACTGGTATTGCTCGTGATTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCAAGGCCGAATAAACAGAGATTGCTCGTACGGTTATCCTAACGCATTGGCTGc                                                                                                                                    >  5:223496/1‑149 (MQ=255)              cAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGc                                                                                                                                    >  6:35119/1‑148 (MQ=255)              cAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGATAACCGAACga                                                                                                                                             >  1:151850/1‑138 (MQ=255)              cAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGATAACCGAACga                                                                                                                                             <  2:151850/139‑2 (MQ=255)               aaGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGGTATCCGAACGCATGGGCTGCCa                                                                                                                                  >  4:78149/1‑149 (MQ=255)                   gtAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAAcc                                                                                                                               >  3:262476/1‑148 (MQ=255)                    tAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTa                                                                                                                             >  3:228942/1‑149 (MQ=255)                            gACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTa                                                                                                                     >  7:190175/1‑149 (MQ=255)                             aCTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGACTCCAGACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCgg                                                                                                                       >  1:97959/1‑148 (MQ=255)                                 gTGCCATGCCCTGCTGTCGACCTTATACCACTGGTATTGCTCGTGCTTGTCAGGCTC‑‑GACCTCTCTCCGGTGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGGCAACCTATCCCGGTATc                                                                                                                   <  6:223496/146‑1 (MQ=255)                                     cAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCg                                                                                                            >  2:307406/1‑149 (MQ=255)                                      aaGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCgg                                                                                                           <  4:262476/149‑1 (MQ=255)                                              ctgTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTa                                                                                                     <  8:150151/147‑1 (MQ=255)                                                 tCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCa                                                                                                 <  1:307406/148‑1 (MQ=255)                                                   gACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTg                                                                                              <  5:198878/149‑1 (MQ=255)                                                      cTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTgcgc                                                                                           >  2:270390/1‑149 (MQ=255)                                                      cTTAAACCACTGGTATTGCTCGCGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGt                                                                                                          >  2:27903/1‑134 (MQ=255)                                                       ttAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGcgcg                                                                                          >  6:74936/1‑149 (MQ=255)                                                             cACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAgg                                                                                    <  8:190175/149‑1 (MQ=255)                                                              aCTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAggg                                                                                   >  2:7719/1‑149 (MQ=255)                                                                       gCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACg                                                                           >  1:24342/1‑148 (MQ=255)                                                                        cTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACg                                                                           >  3:211959/1‑147 (MQ=255)                                                                        cTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTa                                                                         <  1:246953/149‑1 (MQ=255)                                                                         tCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAg                                                                        >  5:4940/1‑149 (MQ=255)                                                                             gatagACAGACTC‑‑GACCTCACTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCAGAACGCATGGGCTGCCAACCTATCCCGGTATCGCATGCTGTAACTATTCAGTGCGCGGCAACGGCTCAg                                                                              >  1:45750/5‑139 (MQ=255)                                                                             gCTTGACAGTCTC‑‑GACCTCTCTGCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGGCATCCTATCGGGGTATCGGTTGCGGTAACTATGCAGTGCGCTGCAAGGGCTCAg                                                                              <  2:45750/139‑1 (MQ=255)                                                                               ttGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCt                                                                  >  8:169863/1‑149 (MQ=255)                                                                               ttGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGAATGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCt                                                                  >  4:159553/1‑149 (MQ=255)                                                                                            gACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCttt                                                         <  2:274527/147‑1 (MQ=255)                                                                                            gACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCttt                                                         <  7:273085/147‑1 (MQ=255)                                                                                             aCCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGcc                                                      <  5:35119/149‑1 (MQ=255)                                                                                              ccTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCt                                                     <  2:24342/149‑1 (MQ=255)                                                                                               ctctctCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTg                                                    >  4:220284/1‑149 (MQ=255)                                                                                                 ctctcCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCt                                                     >  6:155910/1‑146 (MQ=255)                                                                                                 ctctcCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCt                                                     <  5:155910/146‑1 (MQ=255)                                                                                                      cGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCa                                                                               >  8:271045/1‑115 (MQ=255)                                                                                                         ggTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCca                                          <  1:270390/149‑1 (MQ=255)                                                                                                           ttCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCcaca                                        <  4:211959/149‑1 (MQ=255)                                                                                                              gCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGAt                                     <  6:4940/149‑1 (MQ=255)                                                                                                                   gCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGga                                                  <  8:151603/131‑1 (MQ=255)                                                                                                                   gCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGga                                                  >  7:151603/1‑131 (MQ=255)                                                                                                                   gCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTcac                                >  7:197509/1‑149 (MQ=255)                                                                                                                   gCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTcac                                >  4:257268/1‑149 (MQ=255)                                                                                                                      gAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTcaccg                              >  1:149845/1‑148 (MQ=255)                                                                                                                      gAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTcaccg                              >  4:23343/1‑148 (MQ=255)                                                                                                                           aaCAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCgg                        >  6:75495/1‑149 (MQ=255)                                                                                                                            aCAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGc                       >  5:288182/1‑149 (MQ=255)                                                                                                                                  aTTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGa                 <  1:7719/149‑1 (MQ=255)                                                                                                                                   ttGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGaa                <  3:257268/149‑1 (MQ=255)                                                                                                                                   ttGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGaa                <  5:74936/149‑1 (MQ=255)                                                                                                                                     gCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAAc               <  5:75495/148‑1 (MQ=255)                                                                                                                                       gcGTTCGGATAACCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGAt            >  4:272133/2‑149 (MQ=255)                                                                                                                                         gTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGAtt           >  3:23818/1‑148 (MQ=255)                                                                                                                                         gTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTc          <  3:78149/149‑1 (MQ=255)                                                                                                                                            cGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGc                                                              >  6:197403/1‑94 (MQ=255)                                                                                                                                            cGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGc                                                              <  5:197403/94‑1 (MQ=255)                                                                                                                                             ggTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATc      <  3:159553/149‑1 (MQ=255)                                                                                                                                              gTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATc      >  3:320676/1‑148 (MQ=255)                                                                                                                                                tATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATCGa    >  4:35478/1‑148 (MQ=255)                                                                                                                                                  tCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGCCGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATCGATa  <  3:220284/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TGCATTCGGCTACCAAGTGTAGAGCATGACTCGTGCCAAGCCCTGCTGTCGACCTTAAACCACTGGTATTGCTCGTGCTTGACAGTCTC‑‑GACCTCTCTCCGGGGTTCGCCAGGCCGAATAAACAGCGATTGCTCGTTCGGTTATCCGAACGCATGGGCTGCCAACCTATCCCGGTATCGCTTGCGGTAACTATGCAGTGCGCGGCAAGGGCTCAGACGTAGTGAGCTTTGCTCTTTGCCTGGAGAGTTCCACAGATGTCACCGCACCGGCTCCCGAACGATTCCATCGATA  >  NC_002947/1226567‑1226857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑