Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,244,369 C→G 6.9% G39A (GGT→GCT) ‡ rnt ← ribonuclease T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,244,3690CG 6.9% 173.0 / 4.0 58G39A (GGT→GCT) ‡rntribonuclease T
Reads supporting (aligned to +/- strand):  ref base C (24/30);  new base G (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTT  >  NC_002947/1244222‑1244516
                                                                                                                                                   |                                                                                                                                                   
tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAaccacc                                                                                                                                                    >  4:279963/1‑149 (MQ=255)
tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGAATCGGTAGCGCTGTTGAAaccacc                                                                                                                                                    >  6:102893/1‑149 (MQ=255)
  aaGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCgg                                                                                                                                                  >  6:325676/1‑149 (MQ=255)
    gTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGtt                                                                                                                                                <  4:141231/149‑1 (MQ=255)
        ggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCaa                                                                                                                                            >  8:159790/1‑149 (MQ=255)
        ggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCaa                                                                                                                                            >  2:144173/1‑149 (MQ=255)
          ccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                          <  7:159790/149‑1 (MQ=255)
          ccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACa                                                                                                                                          <  2:138862/149‑1 (MQ=255)
            ggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATc                                                                                                                                        <  4:186410/149‑1 (MQ=255)
               tCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                     >  6:59864/1‑149 (MQ=255)
                 gATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                     <  5:325676/147‑1 (MQ=255)
                 gATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                     <  7:58266/147‑1 (MQ=255)
                          gcCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCa                                                                                                                          <  3:279963/149‑1 (MQ=255)
                             cTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCACGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGa                                                                                                                        >  6:273577/1‑148 (MQ=255)
                                  aCGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCaa                                                                                                                                            <  3:204558/123‑1 (MQ=255)
                                  aCGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCaa                                                                                                                                            >  4:204558/1‑123 (MQ=255)
                                       tCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCgg                                                                                                               >  8:100/1‑147 (MQ=255)
                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCggta                                                                                                             <  5:329339/147‑3 (MQ=255)
                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                             <  1:335143/147‑1 (MQ=255)
                                              ggTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCg                                                                                                      <  3:17729/149‑1 (MQ=255)
                                                  gAAGTAGGAATGTTCGGGCAACAGGAAACCTTTATCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTGTCAACATCAACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGcc                                                                                                   >  4:235651/1‑148 (MQ=255)
                                                     gTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCg                                                                                               <  7:266554/149‑1 (MQ=255)
                                                        ggTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGt                                                                                            <  2:17700/149‑1 (MQ=255)
                                                           aTGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGc                                                                                         >  4:62826/1‑149 (MQ=255)
                                                             gTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGACGCGGAAGCGCTCGGTCATCGGGTGGc                                                                                         >  8:183565/1‑147 (MQ=255)
                                                                cGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGTCATCGGGTGGCGAgg                                                                                     <  7:183565/148‑1 (MQ=255)
                                                                 ggggAACAGGAAACCTTTCTCGTCCACGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGAc                                                                                   <  5:273577/149‑1 (MQ=255)
                                                                    gAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGa                                                                                       <  8:246027/142‑1 (MQ=255)
                                                                    gAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGa                                                                                       >  7:246027/1‑142 (MQ=255)
                                                                       cAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCgctgc                                                                             >  7:67927/1‑149 (MQ=255)
                                                                          gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCtgctgc                                                                          >  4:78913/1‑149 (MQ=255)
                                                                          gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCtgctgc                                                                          >  1:266074/1‑149 (MQ=255)
                                                                             aCCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCtt                                                                       <  7:100/149‑1 (MQ=255)
                                                                               cTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                     <  1:144173/149‑1 (MQ=255)
                                                                                    tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTc                                                                >  8:312884/1‑149 (MQ=255)
                                                                                      gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTc                                                                >  2:247937/1‑147 (MQ=255)
                                                                                         cATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgt                                                           >  7:214241/1‑149 (MQ=255)
                                                                                          atccaaaTGGTGACCGCGCCAATATCCAGCAATGCATCGGTAGCCCTGTTGAAACCACCGCTATCAACATCCACCACCACCGCCAGATACCCGCCGAACCGATCCCCCATCGGGTGGCGAGGACCGCTGCTGCCTTCGGTGTCCAGgtc                                                          >  6:178949/6‑149 (MQ=255)
                                                                                                    tGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTACTGCCTTGGGTGTCCAGGTCGTCTTCGTa                                                 >  5:149687/1‑148 (MQ=255)
                                                                                                     gACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTaaa                                               <  6:143392/149‑1 (MQ=255)
                                                                                                         gcggcAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTc                                           <  3:62826/149‑1 (MQ=255)
                                                                                                            gcAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGTCGATGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTc                                        <  5:59864/149‑1 (MQ=255)
                                                                                                                   ccAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTAGCTCAcg                                 >  7:280985/1‑149 (MQ=255)
                                                                                                                     aGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCt                                                                        <  6:74845/108‑1 (MQ=255)
                                                                                                                     aGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCGAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgcg                               >  5:85484/1‑149 (MQ=255)
                                                                                                                     aGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgcg                               >  6:30955/1‑149 (MQ=255)
                                                                                                                     aGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgcg                               <  8:214241/149‑1 (MQ=255)
                                                                                                                     aGCAATGCATCGGTAGCGCTGTTGAAACAACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCt                                                                        >  5:74845/1‑108 (MQ=255)
                                                                                                                        aaTGCATCGGTAGCTCTGTTGAAAGCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTAGGCCATCGGGTGGCTAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCACTCACGCGttt                            <  5:102893/149‑1 (MQ=255)
                                                                                                                        aaTGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGttt                            <  2:266074/149‑1 (MQ=255)
                                                                                                                            cATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGGCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtc                         <  1:247937/148‑1 (MQ=255)
                                                                                                                              tCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTcc                      <  7:312884/149‑1 (MQ=255)
                                                                                                                                ggTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCTGCCATCGGGTGGCGAGGACCGCTACTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCca                     <  6:149687/148‑1 (MQ=255)
                                                                                                                                  tAGTGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCcagca                  <  2:201977/149‑1 (MQ=255)
                                                                                                                                          ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgcgc             >  2:333392/1‑146 (MQ=255)
                                                                                                                                          ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCa           <  1:333392/148‑1 (MQ=255)
                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGttt                            <  2:66896/129‑1 (MQ=255)
                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGttt                            >  1:66896/1‑129 (MQ=255)
                                                                                                                                              aaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcg        >  4:23555/1‑147 (MQ=255)
                                                                                                                                              aaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCAGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAACGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCa      <  1:174311/149‑1 (MQ=255)
                                                                                                                                                 caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtt   >  3:269801/1‑149 (MQ=255)
                                                                                                                                                 caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGCCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtt   >  4:56202/1‑149 (MQ=255)
                                                                                                                                                  accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGttt  >  1:302387/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTT  >  NC_002947/1244222‑1244516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑