Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,253,433 G→C 7.7% intergenic (‑79/+28) PP_1096 ← / ← metG electron transport complex protein/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,253,4330GC 7.7% 166.4 / 3.3 52intergenic (‑79/+28)PP_1096/metGelectron transport complex protein/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

GTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG  >  NC_002947/1253285‑1253577
                                                                                                                                                    |                                                                                                                                                
gTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAgg                                                                                                                                                  <  2:297423/149‑1 (MQ=255)
  ggCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGcc                                                                                                                                                <  6:4914/149‑1 (MQ=255)
    cTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGc                                                                                                                                              <  7:27220/149‑1 (MQ=255)
           aGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTCCcaca                                                                                                                                       <  1:68395/149‑1 (MQ=255)
                gCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGc                                                                                                                                  >  8:225592/1‑149 (MQ=255)
                    aGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCg                                                                                                                               >  8:27754/1‑148 (MQ=255)
                      gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTCCCACAAttacgcag                                                                                                                              <  1:258/147‑8 (MQ=39)
                      gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTCCCACAAttacgcag                                                                                                                              >  2:258/1‑140 (MQ=39)
                             atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGt                                                                                                                      <  1:257680/148‑1 (MQ=255)
                             atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGt                                                                                                                      >  6:56197/1‑148 (MQ=255)
                              tCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTa                                                                                                                    >  8:15815/1‑149 (MQ=255)
                                 gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 <  7:27754/149‑1 (MQ=255)
                                 gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 >  6:6548/1‑149 (MQ=255)
                                 gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 <  3:239445/149‑1 (MQ=255)
                                  tGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTg                                                                                                                <  8:80022/149‑1 (MQ=255)
                                            aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATgcg                                                                                                           >  8:256499/1‑144 (MQ=255)
                                            aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATgcg                                                                                                           <  7:256499/144‑1 (MQ=255)
                                               aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctg                                                                                                   >  6:114489/1‑149 (MQ=255)
                                                 gACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctgg                                                                                                  <  3:33686/148‑1 (MQ=255)
                                                 gACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctgg                                                                                                  >  4:33686/1‑148 (MQ=255)
                                                    cAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTg                                                                                              <  6:305069/149‑1 (MQ=255)
                                                            aGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 >  6:258514/1‑122 (MQ=255)
                                                                   catGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCgg                                                                                <  2:57996/148‑1 (MQ=255)
                                                                    atGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCggg                                                                               <  7:15815/148‑1 (MQ=255)
                                                                             cGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGt                                                                     <  6:292947/149‑1 (MQ=255)
                                                                              gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 >  5:293110/1‑104 (MQ=255)
                                                                              gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                                 <  6:293110/104‑1 (MQ=255)
                                                                              gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAgg                                                                      >  3:31867/1‑147 (MQ=255)
                                                                              gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTa                                                                    >  5:276275/1‑149 (MQ=255)
                                                                              gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTa                                                                    <  6:193521/149‑1 (MQ=255)
                                                                                  gCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGAt                                                                 <  5:6548/148‑1 (MQ=255)
                                                                                      ggAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATttctt                                                            <  5:114489/149‑1 (MQ=255)
                                                                                             gggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGc                                                      >  7:246300/1‑148 (MQ=255)
                                                                                             gggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGc                                                      >  1:338227/1‑148 (MQ=255)
                                                                                              ggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGc                                                      >  4:283359/1‑147 (MQ=255)
                                                                                                 gATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAggg                                                 <  1:339385/149‑1 (MQ=255)
                                                                                                   ttGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGcc                                               >  3:142151/1‑149 (MQ=255)
                                                                                                      tCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGc                                            <  2:338227/149‑1 (MQ=255)
                                                                                                         gggTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGc                                         >  7:133680/1‑149 (MQ=255)
                                                                                                           gTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCa                                       >  3:127996/1‑149 (MQ=255)
                                                                                                            tttGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCAt                                      <  5:141690/149‑1 (MQ=255)
                                                                                                                  aTGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCAt                                >  2:207988/1‑149 (MQ=255)
                                                                                                                         ggCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCg                         <  4:253191/149‑1 (MQ=255)
                                                                                                                          gCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCAt                                >  2:122572/1‑141 (MQ=255)
                                                                                                                          gCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCAt                                <  1:122572/141‑1 (MQ=255)
                                                                                                                           ccTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGa                       <  5:56197/149‑1 (MQ=255)
                                                                                                                                gTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGCTTTCTTCACCGCCAGGGCCGGCAGACATGACCATGCCTTCGGACACGc                  >  3:69414/1‑149 (MQ=255)
                                                                                                                                  caTTGTGGGAGCGGCCTGCCCGCT‑CCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCa                >  6:331815/2‑148 (MQ=255)
                                                                                                                                    ttGTGGTAGCGGGCAGGCAGCTCCCACAATGGCCCGCAGTGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCaaa              <  8:246300/149‑1 (MQ=255)
                                                                                                                                    ttGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCaaa              <  3:283359/149‑1 (MQ=255)
                                                                                                                                               ggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATttcttc                                                           >  2:280453/1‑93 (MQ=255)
                                                                                                                                               cgCAGGCGGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATttcttc                                                           <  1:280453/92‑1 (MQ=255)
                                                                                                                                                gCAGGCCGCTCCCACAATGGCCCGCAGGCCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCg  >  5:62722/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG  >  NC_002947/1253285‑1253577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑