BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,346	T→G	7.1%	T13P (ACG→CCG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,346	0	T	G	7.1%	312.8 / 8.6	104	T13P (ACG→CCG)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base T (49/46); new base G (4/4); total (54/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGC  >  NC_002947/6200‑6494

gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGGGCGGCCGGTGATCAACCGGCCGGCCTTGGCAGCCAGACGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTgg                                                                                                                                                     <  7:227279/149‑1 (MQ=255)

gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTg                                                                                                                                                      <  7:67333/148‑1 (MQ=255)

   cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCg                                                                                                                                                   <  2:30873/148‑1 (MQ=255)

   cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGa                                                                                                                                                  >  7:159713/1‑149 (MQ=255)

   cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGa                                                                                                                                                  <  7:33387/149‑1 (MQ=255)

     gCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCATGGTGCGGCCGGTGATCAACAGGGCGGCCTTGGCAGCCAGAGGGCCGTACATCCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATg                                                                                                                                                <  1:278521/149‑1 (MQ=255)

       aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATggc                                                                                                                                              >  3:325303/1‑149 (MQ=255)

       aCCAGCCCTTCATCGGCGCGGAATCGACCTTACTGGGCATGCCGCGGGGTCTGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCA‑GGGCCGGGGCGGTgtg                                                                                                                                              <  8:277901/149‑3 (MQ=255)

        ccAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATggcg                                                                                                                                             >  6:281070/1‑149 (MQ=255)

         cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATggcgg                                                                                                                                            <  1:26769/149‑1 (MQ=255)

            cccTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGa                                                                                                                                         <  6:341582/149‑1 (MQ=255)

              cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCA‑GGGCCGGGGCGGTgtgg                                                                                                                                             >  6:85579/1‑140 (MQ=255)

              cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATg                                                                                                                                       >  4:65266/1‑149 (MQ=255)

               ttCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATgg                                                                                                                                      >  7:138366/1‑149 (MQ=255)

                 cATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATgg                                                                                                                                      <  4:93739/147‑1 (MQ=255)

                      tcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                               >  8:170364/1‑149 (MQ=255)

                        gcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCA‑GGGCCGGGGCGGTgtg                                                                                                                                              >  5:109539/1‑130 (MQ=255)

                        gcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCA‑GGGCCGGGGCGGTgtg                                                                                                                                              <  6:109539/132‑3 (MQ=255)

                          ggAATGGACCGTAATGGGCATGCGGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca                                                                                                                           <  6:36022/149‑1 (MQ=255)

                            aaTGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgt                                                                                                                         >  1:225557/1‑149 (MQ=255)

                                 aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                                    <  7:170364/149‑1 (MQ=255)

                                 aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                                    <  3:215499/149‑1 (MQ=255)

                                   cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATg                                                                                                                  >  5:185134/1‑149 (MQ=255)

                                   cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCA‑GGGCCGGGGCGGTgtg                                                                                                                                              >  8:160067/1‑119 (MQ=255)

                                    gTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGc                                                                                                                 <  8:159713/149‑1 (MQ=255)

                                     tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCt                                                                                                                >  3:101658/1‑149 (MQ=255)

                                      aaTGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACATGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                               >  8:228431/1‑149 (MQ=255)

                                                ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                                      <  2:40300/148‑1 (MQ=255)

                                                ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                                      >  8:315077/1‑148 (MQ=255)

                                                  gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                                   <  3:21135/149‑1 (MQ=255)

                                                  gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                                   <  5:281070/149‑1 (MQ=255)

                                                  gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                                   <  8:45947/149‑1 (MQ=255)

                                                     gggTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGAc                                                                                                <  3:65266/149‑1 (MQ=255)

                                                          aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaaa                                                                                           >  8:219990/1‑149 (MQ=255)

                                                          aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaaa                                                                                           <  7:228431/149‑1 (MQ=255)

                                                            ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgtgt                                                                                                                       >  3:212246/1‑119 (MQ=255)

                                                            ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgtgt                                                                                                                       <  4:212246/119‑1 (MQ=255)

                                                             gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                                        <  7:219990/149‑1 (MQ=255)

                                                              tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACTAAAACGAc                                                                                       <  4:39275/149‑1 (MQ=255)

                                                                   ccGGTGATCAACAGGCCGACCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATa                                                                                  <  7:49598/149‑1 (MQ=255)

                                                                      gTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGc                                                                                >  4:7823/1‑148 (MQ=255)

                                                                        gATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaa                                                                              >  3:181262/1‑148 (MQ=255)

                                                                          tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaaaa                                                                           <  4:325303/149‑1 (MQ=255)

                                                                          tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaaaa                                                                           >  5:182017/1‑149 (MQ=255)

                                                                           cAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                                          >  1:216668/1‑149 (MQ=255)

                                                                              cAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                                       <  2:114555/149‑1 (MQ=255)

                                                                                 gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                                    >  1:243904/1‑149 (MQ=255)

                                                                                   cggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                                      <  4:288868/113‑1 (MQ=255)

                                                                                     gccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg                                                                >  7:244993/1‑149 (MQ=255)

                                                                                          ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑ggg                                                            <  2:225557/147‑1 (MQ=255)

                                                                                          ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGc                                                          >  8:120455/1‑149 (MQ=255)

                                                                                          ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAg‑tggg                                                           <  8:138366/147‑1 (MQ=255)

                                                                                           gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                                 >  1:139010/1‑110 (MQ=255)

                                                                                           gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                                 <  2:139010/110‑1 (MQ=255)

                                                                                           gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAACCGACAGATAGCAAAAAAGCCCCACTAG‑GGGGCg                                                         >  2:238643/1‑149 (MQ=255)

                                                                                           gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCg                                                         >  6:210340/1‑149 (MQ=255)

                                                                                               ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttt                                                     >  3:124843/1‑149 (MQ=255)

                                                                                                cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGttttt                                                    >  8:265029/1‑149 (MQ=255)

                                                                                                  gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑CGGGCGTTTTTTa                                                  <  2:216668/149‑1 (MQ=255)

                                                                                                     ggCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAATAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTc                                               <  1:238643/149‑1 (MQ=255)

                                                                                                        cGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGttttt                                                    >  4:213091/1‑141 (MQ=255)

                                                                                                        cGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGttttt                                                    <  3:213091/141‑1 (MQ=255)

                                                                                                            cAACCTGACAATTCCCACACCGCCCCGGCCCT‑CGGCCGAGGCGATGGCGGCGATGGTTTCACGCACAGTGTTAATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGAGTTTATTATTAACAGGc                                         >  3:67362/1‑148 (MQ=255)

                                                                                                               ccTGACAATTCCCACACCGCCCCGGCCCTAGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAg                                      >  1:198424/1‑149 (MQ=255)

                                                                                                               ccTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGc                                     <  7:120455/149‑1 (MQ=255)

                                                                                                               ccTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGc                                     >  3:89307/1‑149 (MQ=255)

                                                                                                                 tGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                                    >  3:159014/1‑148 (MQ=255)

                                                                                                                 tGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGATCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                                    >  8:274597/1‑148 (MQ=255)

                                                                                                                  gACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                                    >  7:188541/1‑147 (MQ=255)

                                                                                                                  gACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTa                                  >  2:337948/1‑149 (MQ=255)

                                                                                                                  gACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGtttttgcta                                                >  1:315053/1‑131 (MQ=255)

                                                                                                                  gACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGtttttgcta                                                <  2:315053/135‑5 (MQ=255)

                                                                                                                     aaTTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGGTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAt                                 >  8:252764/1‑147 (MQ=255)

                                                                                                                       ttCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc                             >  2:253554/1‑149 (MQ=255)

                                                                                                                        tCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAACGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGcc                            >  5:237343/1‑149 (MQ=255)

                                                                                                                          ccACTCCGCCGCGGCGCT‑GGGCCGTGGCGATGGCGGCGTTGGTTTCACGCACTGTGTTCATGCTCGAAAGCCTCTACGTCAAAATCGACAGTTAGCAAATACGCCCCACTTG‑GTGGCGTTTTTTATTGACTGGCTAGCGTa                                  <  4:276222/141‑1 (MQ=255)

                                                                                                                          ccACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTa                                  >  3:276222/1‑141 (MQ=255)

                                                                                                                          ccACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc                           <  2:198424/148‑1 (MQ=255)

                                                                                                                          ccACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc                           <  4:89307/148‑1 (MQ=255)

                                                                                                                          ccACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                          >  2:219095/1‑149 (MQ=255)

                                                                                                                          ccACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                          >  7:23476/1‑149 (MQ=255)

                                                                                                                           cacaCCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt                         >  5:302907/1‑149 (MQ=255)

                                                                                                                            ccatcGCCACGGCCCA‑GGGCCGGGGCGGTGTGGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc                        <  7:22846/145‑1 (MQ=255)

                                                                                                                              acCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAGACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc                        >  6:303548/1‑147 (MQ=255)

                                                                                                                              acCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGGTTTTGATTCACAGGCTAGCGTATTAGCCCGTc                        >  6:146713/1‑147 (MQ=255)

                                                                                                                               ccGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag                     <  7:265029/149‑1 (MQ=255)

                                                                                                                               ccGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag                     <  4:124843/149‑1 (MQ=255)

                                                                                                                                cGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc                    <  8:244993/149‑1 (MQ=255)

                                                                                                                                 gCCCCGGCCCT‑GGGCCGTGGCGATGGCTGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTGACAGGCTAGCGTATTAGCCCGTCAagca                   <  4:67362/149‑1 (MQ=255)

                                                                                                                                 gCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTcaca                                            >  3:238725/1‑124 (MQ=255)

                                                                                                                                 gCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTcaca                                            <  4:238725/124‑1 (MQ=255)

                                                                                                                                 gCCACGGCCCA‑GGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag                     >  3:226433/1‑147 (MQ=255)

                                                                                                                                  ccccGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag                  >  1:329236/1‑149 (MQ=255)

                                                                                                                                           t‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaaa                                                                                           <  7:276202/68‑1 (MQ=255)

                                                                                                                                           t‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaaa                                                                                           >  8:276202/1‑68 (MQ=255)

                                                                                                                                             gggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGc                                         >  3:156219/1‑116 (MQ=255)

                                                                                                                                             gggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGc                                         <  4:156219/116‑1 (MQ=255)

                                                                                                                                              ggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCttttt         <  6:185134/147‑1 (MQ=255)

                                                                                                                                              ggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTg       <  1:253554/149‑1 (MQ=255)

                                                                                                                                                   tGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgc  <  2:243904/149‑1 (MQ=255)

GTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCT‑GGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGC  >  NC_002947/6200‑6494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`